Using HPLC–DAD and GC–MS Analysis Isolation and Identification of Anticandida Compounds from Gui Zhen Cao Herbs (Genus Bidens): An Important Chinese Medicinal Formulation

Abstract

Gui Zhen Cao is an herbal formulation that has been documented in Chinese traditional medicine as a remedy for diarrhea, dysentery, inflammation, and toxicity. The sources of this formulation (Bidens pilosa L., Bidens biternata (Lour.) Merr. & Sherff, Bidens bipinnata L.) are also listed in ethnomedicinal reports all over the world. In this study, all these plants are tested for in vitro anticandida activity. A quantitative evaluation of the phytochemicals in all these plants indicated that their vegetative parts are rich in tannins, saponins, oxalates, cyanogenic glycoside and lipids; moreover, the roots have high percentages of alkaloids, flavonoids, and phenols. The results indicated significant anticandida activity, especially for the hexane extract of B. bipinnata leaves which inhibited C. albicans (42.54%), C. glabrata (46.98%), C. tropicalis (50.89%), C. krusei (40.56%), and C. orthopsilosis (50.24%). The extract was subjected to silica gel chromatography and 220 fractions were obtained. Purification by High Performance Liquid Chromatography with Diode-Array Detection (HPLC–DAD) and Gas Chromatography tandem Mass Spectrometry (GC-MS/MS) analysis led to the identification of two anticandida compounds: dehydroabietic and linoleic acid having an inhibition of 85 and 92%, respectively.

Quality evaluation methods of chinese medicine based on scientific supervision: recent research progress and prospects

“…HPLC-high resolution MS and tandem MS (HPLC-HRMS/MS), ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) [ 18 ]. Additionally, other methods are used to qualitatively and quantitatively identify and determine the active ingredients in CM, such as nuclear magnetic resonance (NMR) [ 19 ], and gas chromatography-mass spectrometry (GC–MS) [ 20 ]. These methods are generally considered simple, effective, innovative, high separation efficiency, high sensitivity, low potential interference, and short run time, thereby contributing to the development of quality control.…”

Rapid analysis of differential chemical compositions of Poria cocos using thin-layer chromatography spray ionization-mass spectrometry

“…Commonly used analytical methods include thin-layer chromatography (TLC), 8 high-performance liquid chromatography-mass spectrometry (HPLC-MS), 9 gas chromatography (GC), 10 and nuclear magnetic resonance (NMR). 11 Of these, HPLC-MS has been used to analyze the chemical composition of natural herbal medicines due to its efficient separation and analytical capabilities.…”