Using high-throughput transcriptome sequencing to investigate the biotransformation mechanism of hexabromocyclododecane with Rhodopseudomonas palustris in water

Wang, Reuben; Lin, Chung Yen; Chen, Shu Hwa; Lo, Kai-Jiun; Liu, Chi Te; Chou, Teh‐Chang; Shih, Yung‐Feng

doi:10.1016/j.scitotenv.2019.07.140

Cited by 18 publications

(6 citation statements)

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“…We screened our data for these two genes and while we did not detect homologues of the gene encoding 2-haloacid dehydrogenase (K01560), we found genes for glutathione S -transferase (K00799) in all feeding trials and faeces samples (Table S9). Since both enzymes are thought to work together sequentially [112], we conclude that an R. palustris- like pathway of HBCD degradation might not be present in the superworm gut microbiome. The presence of glutathione S -transferase genes in all our samples further suggests that this glutathione transferase is widely distributed in prokaryotes and is involved in a variety of processes, as suggested previously [113].…”

Section: Resultsmentioning

confidence: 99%

“…aeruginosa HS9 and Rhodopseudomonas palustris , were reported to effectively degrade HBCD [110]. While most chemical compounds produced during bacterial HBCD degradation have been studied in detail [111, 112], only two enzymes, a dehydrogenase and a transferase, involved in catalysing these reactions have been identified in R. palustris [112].…”

Section: Resultsmentioning

confidence: 99%

“…HB01, P. aeruginosa HS9 and Rhodopseudomonas palustris, were reported to effectively degrade HBCD [110]. While most chemical compounds produced during bacterial HBCD degradation have been studied in detail [111,112], only two enzymes, a dehydrogenase and a transferase, involved in catalysing these reactions have been identified in R. palustris [112]. We screened our data for these two genes and while we did not detect homologues of the gene encoding 2-haloacid dehydrogenase (K01560), we found genes for glutathione S-transferase (K00799) in all feeding trials and faeces samples (Table S9).…”

Section: Alternative Microbial Carbon Sources During Ps Feeding Trialsmentioning

confidence: 99%

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Insights into plastic biodegradation: community composition and functional capabilities of the superworm (Zophobas morio) microbiome in styrofoam feeding trials

2022

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Plastics are inexpensive and widely used organic polymers, but their high durability hinders biodegradation. Polystyrene, including extruded polystyrene (also known as styrofoam), is among the most commonly produced plastics worldwide and is recalcitrant to microbial degradation. In this study, we assessed changes in the gut microbiome of superworms (Zophobas morio) reared on bran, polystyrene or under starvation conditions over a 3 weeks period. Superworms on all diets were able to complete their life cycle to pupae and imago, although superworms reared on polystyrene had minimal weight gains, resulting in lower pupation rates compared to bran reared worms. The change in microbial gut communities from baseline differed considerably between diet groups, with polystyrene and starvation groups characterized by a loss of microbial diversity and the presence of opportunistic pathogens. Inferred microbial functions enriched in the polystyrene group included transposon movements, membrane restructuring and adaptations to oxidative stress. We detected several encoded enzymes with reported polystyrene and styrene degradation abilities, supporting previous reports of polystyrene-degrading bacteria in the superworm gut. By recovering metagenome-assembled genomes (MAGs) we linked phylogeny and functions and identified genera including Pseudomonas , Rhodococcus and Corynebacterium that possess genes associated with polystyrene degradation. In conclusion, our results provide the first metagenomic insights into the metabolic pathways used by the gut microbiome of superworms to degrade polystyrene. Our results also confirm that superworms can survive on polystyrene feed, but this diet has considerable negative impacts on host gut microbiome diversity and health.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Alternative Microbial Carbon Sources During Ps Feeding Trialsmentioning

confidence: 99%

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Insights into plastic biodegradation: community composition and functional capabilities of the superworm (Zophobas morio) microbiome in styrofoam feeding trials

2022

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“…We did not detect genes encoding 2-haloacid dehydrogenase (K01560) in our data but found genes for glutathione S-transferase (K00799) in all feeding trials and feces samples ( Table S9 ). Since both enzymes are thought to work together sequentially (Wang et al, 2019), we conclude that an R. palustris- like pathway of HBCD degradation might not be present in the superworm gut microbiome. The presence of glutathione S-transferase genes in all our samples further suggests that this glutathione transferase is widely distributed in prokaryotes and is involved in a variety of processes, as suggested previously (Allocati et al, 2009).…”

Section: Resultsmentioning

confidence: 94%

“…HB01, P. aeruginosa HS9, and Rhodopseudomonas palustris were reported to effectively degrade HBCD (Li et al, 2021). While most chemical compounds produced during bacterial HBCD degradation have been studied in detail (Huang et al, 2019; Wang et al, 2019), only two enzymes, a dehydrogenase and a transferase, involved in catalyzing these reactions have been identified in R. palustris (Wang et al, 2019). We did not detect genes encoding 2-haloacid dehydrogenase (K01560) in our data but found genes for glutathione S-transferase (K00799) in all feeding trials and feces samples ( Table S9 ).…”

Section: Resultsmentioning

confidence: 99%

Insights into plastic biodegradation: community composition and functional capabilities of the superworm (Zophobas morio) microbiome in styrofoam feeding trials

Sun

Prabhu

Aroney

2022

Preprint

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Plastics are inexpensive and widely used organic polymers, but their high durability hinders biodegradation. Polystyrene, including extruded polystyrene also known as styrofoam, is among the most commonly produced plastics worldwide and is recalcitrant to microbial degradation. In this study, we assessed changes in the gut microbiome of superworms (Zophobas morio) reared on bran, polystyrene, or under starvation conditions over a three weeks’ time period. Superworms on all diets were able to complete their life cycle to pupae and imago, although superworms reared on polystyrene had minimal weight gains, resulting in lower pupation rates. The change in microbial gut communities from baseline differed considerably between diet groups, with polystyrene and starvation groups characterized by a loss of microbial diversity and the presence of opportunistic pathogens. Inferred microbial functions enriched in the polystyrene group included transposon movements, membrane restructuring, and adaptations to oxidative stress. We detected several encoded enzymes with reported polystyrene and styrene degradation abilities, supporting previous reports of polystyrene degrading bacteria in the superworm gut. By recovering metagenome-assembled genomes (MAGs) we linked phylogeny and functions and identified genera including Pseudomonas, Rhodococcus and Corynebacterium, that possess genes associated with polystyrene degradation. In conclusion, our results provide the first metagenomic insights into the metabolic pathways used by the gut microbiome of superworms to degrade polystyrene. Our results also confirm that superworms can survive on polystyrene feed, however, this diet has considerable negative impacts on host gut microbiome diversity and health.Impact StatementIncreasing plastic pollution is a major environmental problem, and a recently proposed way to counteract this trend is to embrace a circular economy, in which used materials are recycled, rather than disposed of. An important step to facilitate this process is to invent new approaches for upcycling of plastic waste to desirable consumer products. Microbial plastic degradation and conversion is likely to play a considerable part in shaping a circular economy, by engineering microbes or their enzymes to bio-upcycle plastic waste. A first step towards actualizing this goal is to identify microbes that can degrade polystyrene and to investigate the enzymes and pathways involved. Our study represents the first metagenomic analysis of an insect gut microbiome on a polystyrene diet. It identifies bacteria with polystyrene and styrene degrading abilities, and infers enzymes and pathways involved in these reactions. Therefore, our results contribute towards understanding microbial polystyrene degradation and will provide a base for future investigations into microbial upcycling of plastic waste.

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Various Disinfection Processes and Formation of Disinfection by-Products in Drinking Water

Zambelli,

de França

2024

Drinking Water Disinfection by-Products

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Using high-throughput transcriptome sequencing to investigate the biotransformation mechanism of hexabromocyclododecane with Rhodopseudomonas palustris in water

Cited by 18 publications

References 46 publications

Insights into plastic biodegradation: community composition and functional capabilities of the superworm (Zophobas morio) microbiome in styrofoam feeding trials

Insights into plastic biodegradation: community composition and functional capabilities of the superworm (Zophobas morio) microbiome in styrofoam feeding trials

Insights into plastic biodegradation: community composition and functional capabilities of the superworm (Zophobas morio) microbiome in styrofoam feeding trials

Various Disinfection Processes and Formation of Disinfection by-Products in Drinking Water

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