Using Foldscope to Monitor Superoxide Production and Cell Death During Pathogen Infection in Arabidopsis Under Different Nitrogen Regimes

Arora, Reena; Singh, Pooja; Kumari, Arti; Pathak, Pradeep Kumar; Gupta, Kapuganti Jagadis

doi:10.1007/978-1-4939-9790-9_9

Cited by 2 publications

(2 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the second group of parental genes, the GO enrichment showed that these genes are related to organonitrogen compound biosynthetic and metabolic processing ( Supplementary Figure 2B ). Nitrogen is essential for plant growth and development and is also helpful for tolerance against biotic and abiotic stresses (Oh et al, 2017 ; Zipfel and Oldroyd, 2017 ; Arora et al, 2020 ). Notably, Zm00001d034602 is annotated to function in the organonitrogen compound metabolic process, and its abundance in the translatome was significantly associated with that of the sPeptide Zm00001d034602_T001 ( Figure 4D ).…”

Section: Resultsmentioning

confidence: 99%

“…Signal molecules, such as reactive oxygen species, calcium, reactive nitrogen species, salicylic acid, and ethylene, are important in plant development and pathogen infection (Oh et al, 2017 ; Zipfel and Oldroyd, 2017 ). The form of nitrogen, such as nitrate or ammonium, plays a vital role in the production of these signal molecules (Oh et al, 2017 ; Arora et al, 2020 ). The parental genes of the Group II sPeptide, Zm00001d013642_T001 and Zm00001d013642 , were annotated in “organonitrogen compound metabolic processing,” and its ortholog AT2G22425 was differentially expressed in Cabbage leaf curl virus -infected Arabidopsis leaves (Ascencio-Ibáñez et al, 2008 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genome-Wide Identification and Characterization of Small Peptides in Maize

et al. 2021

View full text Add to dashboard Cite

Small peptides (sPeptides), <100 amino acids (aa) long, are encoded by small open reading frames (sORFs) often found in the 5′ and 3′ untranslated regions (or other parts) of mRNAs, in long non-coding RNAs, or transcripts from introns and intergenic regions; various sPeptides play important roles in multiple biological processes. In this study, we conducted a comprehensive study of maize (Zea mays) sPeptides using mRNA sequencing, ribosome profiling (Ribo-seq), and mass spectrometry (MS) on six tissues (each with at least two replicates). To identify maize sORFs and sPeptides from these data, we set up a robust bioinformatics pipeline and performed a genome-wide scan. This scan uncovered 9,388 sORFs encoding peptides of 2–100 aa. These sORFs showed distinct genomic features, such as different Kozak region sequences, higher specificity of translation, and high translational efficiency, compared with the canonical protein-coding genes. Furthermore, the MS data verified 2,695 sPeptides. These sPeptides perfectly discriminated all the tissues and were highly associated with their parental genes. Interestingly, the parental genes of sPeptides were significantly enriched in multiple functional gene ontology terms related to abiotic stress and development, suggesting the potential roles of sPeptides in the regulation of their parental genes. Overall, this study lays out the guidelines for genome-wide scans of sORFs and sPeptides in plants by integrating Ribo-seq and MS data and provides a more comprehensive resource of functional sPeptides in maize and gives a new perspective on the complex biological systems of plants.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Characterization of Small Peptides in Maize

et al. 2021

View full text Add to dashboard Cite

show abstract

Foldscope Embedded Pedagogy in Stem Education: A Case Study of SDG4 Promotion in India

et al. 2022

View full text Add to dashboard Cite

Most government schools and colleges run on low budgets in India, yet they cater to a large population of students. In government settings, biology labs lack basic equipment such as simple microscopes or compound microscopes or sometimes possess just one instrument. In the absence of compound or simple microscopes, the students lose interest in science. To solve this issue, the Department of Biotechnology, Ministry of Science and Technology, Government of India, introduced the origami microscope, the foldscope, into the Indian educational system. In this article, we describe the design of a sustainable, inclusive, and equitable curricula for teaching biological concepts using the foldscope. We describe the implementation of foldscope-adapted curricula in post-secondary settings to teach natural selection, developmental biology, parasitology, and economic Zoology via individual, small-group, and large-group field trips, and project-based learning that involves experiential learning. We found that these culturally sensitive pedagogies involving translingual instructions had the potential to retain students and make science education accessible for the linguistically and culturally diverse population of India. We could successfully implement our project as per the five priority action areas outlined by UNESCO. Therefore, we propose the adoption of the foldscope-adapted curricula under SDG4 to make STEM education accessible in budget-frugal settings.

show abstract

Using Foldscope to Monitor Superoxide Production and Cell Death During Pathogen Infection in Arabidopsis Under Different Nitrogen Regimes

Cited by 2 publications

References 28 publications

Genome-Wide Identification and Characterization of Small Peptides in Maize

Genome-Wide Identification and Characterization of Small Peptides in Maize

Foldscope Embedded Pedagogy in Stem Education: A Case Study of SDG4 Promotion in India

Contact Info

Product

Resources

About