Using fermentation waste of ethanol-producing yeast for bacterial riboflavin production and recycling of spent bacterial mass for enhancing the growth of oily plants

Abstract

Aim: This study aims to use fermentation waste of ethanol production (solid and liquid) for riboflavin and recycling of bacterial biomass as biofertilizers to enhance the growth of some oily crop plants. Methods and Results:Out of 10 yeast isolates from fresh milk, Clavispora lusitaniae ASU 33 (MN583181) was able to ferment different concentrations of glucose (2.5%, 5%, 7.5%, 10%, 15% and 20%) into ethanol with high efficiency at 10%. Among seven non-Lactobacillus bacterial isolates recovered from cheese samples, two bacterial isolates Bacillus subtlis-SR2 (MT002768) and Novosphingobium panipatense-SR3 (MT002778) were selected for their high riboflavin production. Different media (control medium, fermentation waste medium and a mixture of the fermentation waste medium and control medium [1:1]) were used for riboflavin production. These media were inoculated by a single or mixture of B. subtlis-SR2, N. panipatense-SR3. The addition of the waste medium of ethanol production to the control medium (1:1) had a stimulatory effect on riboflavin production whether inoculated with either a single strain or a mixture of B. subtlis-SR2 and N. panipatense-SR3. A mixture of fermentation waste and control media inoculated with N. panipatense produced a high riboflavin yield in comparison with other media. Inoculation of Zea mays and Ocimum basilicum plants with either the bacterial biomass waste of riboflavin production (B. subtlis or N. panipatense) or a mixture of B. subtlis and N. panipatense) shows a stimulatory effect on the plant growth in comparison with control (uninoculated plants). Conclusions: These results demonstrate the possibility of minimizing the cost of riboflavin and biofertilizer manufacturing via interlinking ethanol and riboflavin with the biofertilizer production technology. Significance and Impact of Study: This study outlines the methods of evaluating the strength of spent media by applying procedures developed in the vitamin production industries. Furthermore, bacterial biomass waste can act as an environmentally friendly alternative for agrochemicals.

Responses of Pea (Pisum sativum L.) to Single and Consortium Bio-Fertilizers in Clay and Newly Reclaimed Soils

“…According to the literature, PGPB is now employed in agriculture, in the cycling of minerals and biofertilizers, as a tool for sustainable agriculture [9]. The prevalent PGPB bacterial genera used in agriculture include Bacillus, Azotobacter, Arthrobacter, Acinetobacter, Beijerinckia, Derxia, Klebsiella, Enterobacter, Serratia, Gluconacetobacter, Alcaligenes, Pseudomonas, Azospirillum, Paenobacillus, and Lactobacillus [10][11][12].…”

Effect of temperature on fermentative VFAs production from waste sludge stimulated by riboflavin and the shifts of microbial community

“…Riboflavin is a biogenic biocatalyst which could be industrially produced by fermentation with waste organic substrates (Ryhan Bashandy et al 2021). In biological systems, riboflavin has been reported to serve as an extracellular redox mediator to transfer electrons between redox reactions, promoting enzymatic activities, biofilm formation, and pollutants removal (Edel et al 2021).…”