Using experimental evolution to explore natural patterns between bacterial motility and resistance to bacteriophages

Koskella, Britt; Taylor, Tiffany B.; Bates, J. A.; Buckling, Angus

doi:10.1038/ismej.2011.47

Cited by 28 publications

(37 citation statements)

References 65 publications

(77 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is probably related to this polysaccharide being the recognition target for many phages70. Interestingly a similar level of variation was found in the giant protein and the flagellum glycosylation Island what might be also related to their potential recognition by phages484950515253545556575859606162636465666768697071. A very peculiar genomic island that was found to be different in all the isolates is the one apparently involved in metal resistance.…”

Section: Discussionmentioning

confidence: 69%

Genomes of surface isolates of Alteromonas macleodii: the life of a widespread marine opportunistic copiotroph

López‐Pérez

Gonzaga

Martín-Cuadrado

et al. 2012

Sci Rep

117

135

View full text Add to dashboard Cite

Alteromonas macleodii is a marine gammaproteobacterium with widespread distribution in temperate or tropical waters. We describe three genomes of isolates from surface waters around Europe (Atlantic, Mediterranean and Black Sea) and compare them with a previously described deep Mediterranean isolate (AltDE) that belongs to a widely divergent clade. The surface isolates are quite similar, the most divergent being the Black Sea (BS11) isolate. The genomes contain several genomic islands with different gene content. The recruitment of very similar genomic fragments from metagenomes in different locations indicates that the surface clade is globally abundant with little effect of geography, even the AltDE and the BS11 genomes recruiting from surface samples in open ocean locations. The finding of CRISPR protospacers of AltDE in a lysogenic phage in the Atlantic (English Channel) isolate illustrates a flow of genetic material among these clades and a remarkably wide distribution of this phage.

show abstract

Section: Discussionmentioning

confidence: 69%

Genomes of surface isolates of Alteromonas macleodii: the life of a widespread marine opportunistic copiotroph

López‐Pérez

Gonzaga

Martín-Cuadrado

et al. 2012

Sci Rep

117

135

View full text Add to dashboard Cite

show abstract

“…Flagellatropic phages are known to reversibly bind to helical grooves on the bacterial flagellum and use the rotation of the flagellum to spiral toward the cell surface (Samuel et al 1999). Any change in flagellin structure or glycosylation would alter phage susceptibility (Koskella et al 2011). Interestingly, we found an intermediate level of variation in genes involved in the regulation of flagellum synthesis.…”

Section: Resultsmentioning

confidence: 99%

Polyclonality of Concurrent Natural Populations of Alteromonas macleodii

Gonzaga

Martín-Cuadrado

López‐Pérez

et al. 2012

Genome Biology and Evolution

View full text Add to dashboard Cite

We have analyzed a natural population of the marine bacterium, Alteromonas macleodii, from a single sample of seawater to evaluate the genomic diversity present. We performed full genome sequencing of four isolates and 161 metagenomic fosmid clones, all of which were assigned to A. macleodii by sequence similarity. Out of the four strain genomes, A. macleodii deep ecotype (AltDE1) represented a different genome, whereas AltDE2 and AltDE3 were identical to the previously described AltDE. Although the core genome (∼80%) had an average nucleotide identity of 98.51%, both AltDE and AltDE1 contained flexible genomic islands (fGIs), that is, genomic islands present in both genomes in the same genomic context but having different gene content. Some of the fGIs encode cell surface receptors known to be phage recognition targets, such as the O-chain of the lipopolysaccharide, whereas others have genes involved in physiological traits (e.g., nutrient transport, degradation, and metal resistance) denoting microniche specialization. The presence in metagenomic fosmids of genomic fragments differing from the sequenced strain genomes, together with the presence of new fGIs, indicates that there are at least two more A. macleodii clones present. The availability of three or more sequences overlapping the same genomic region also allowed us to estimate the frequency and distribution of recombination events among these different clones, indicating that these clustered near the genomic islands. The results indicate that this natural A. macleodii population has multiple clones with a potential for different phage susceptibility and exploitation of resources, within a seemingly unstructured habitat.

show abstract

“…Based on genetic analyses of domestication traits, Fuller & Allaby () inferred that domestication first imposed selection on seed size and germination even before agriculture was widely practiced, and as crops became cultivated, selection for lack of dispersal followed. Similarly in bacteria, experimental evolution and selection experiments show that bacterial motility, as well as sporulation, are evolvable traits (Girgis et al, ; Brown et al, ; Koskella et al, ; Taylor & Buckling, ). Mobility was also successfully selected for in the nematode Caenorhabditis elegans (Friedenberg, ), in a protist (Fronhofer & Altermatt, ), and in different mite species (Gu & Danthanarayana, ; Knülle, ; Nachappa et al, ; Bal, Michel & Grewal, ).…”

Section: Empirical Evidence For the Genetic Basis Of Dispersalmentioning

confidence: 99%

Genetics of dispersal

et al. 2017

View full text Add to dashboard Cite

Dispersal is a process of central importance for the ecological and evolutionary dynamics of populations and communities, because of its diverse consequences for gene flow and demography. It is subject to evolutionary change, which begs the question, what is the genetic basis of this potentially complex trait? To address this question, we (i) review the empirical literature on the genetic basis of dispersal, (ii) explore how theoretical investigations of the evolution of dispersal have represented the genetics of dispersal, and (iii) discuss how the genetic basis of dispersal influences theoretical predictions of the evolution of dispersal and potential consequences.Dispersal has a detectable genetic basis in many organisms, from bacteria to plants and animals. Generally, there is evidence for significant genetic variation for dispersal or dispersal‐related phenotypes or evidence for the micro‐evolution of dispersal in natural populations. Dispersal is typically the outcome of several interacting traits, and this complexity is reflected in its genetic architecture: while some genes of moderate to large effect can influence certain aspects of dispersal, dispersal traits are typically polygenic. Correlations among dispersal traits as well as between dispersal traits and other traits under selection are common, and the genetic basis of dispersal can be highly environment‐dependent.By contrast, models have historically considered a highly simplified genetic architecture of dispersal. It is only recently that models have started to consider multiple loci influencing dispersal, as well as non‐additive effects such as dominance and epistasis, showing that the genetic basis of dispersal can influence evolutionary rates and outcomes, especially under non‐equilibrium conditions. For example, the number of loci controlling dispersal can influence projected rates of dispersal evolution during range shifts and corresponding demographic impacts. Incorporating more realism in the genetic architecture of dispersal is thus necessary to enable models to move beyond the purely theoretical towards making more useful predictions of evolutionary and ecological dynamics under current and future environmental conditions. To inform these advances, empirical studies need to answer outstanding questions concerning whether specific genes underlie dispersal variation, the genetic architecture of context‐dependent dispersal phenotypes and behaviours, and correlations among dispersal and other traits.

show abstract

Using experimental evolution to explore natural patterns between bacterial motility and resistance to bacteriophages

Cited by 28 publications

References 65 publications

Genomes of surface isolates of Alteromonas macleodii: the life of a widespread marine opportunistic copiotroph

Genomes of surface isolates of Alteromonas macleodii: the life of a widespread marine opportunistic copiotroph

Polyclonality of Concurrent Natural Populations of Alteromonas macleodii

Genetics of dispersal

Contact Info

Product

Resources

About