Using eDNA to simultaneously detect the distribution of native and invasive crayfish within an entire country

King, Alex C.; Krieg, Raphael; Weston, Anna; Zenker, Armin

doi:10.1016/j.jenvman.2021.113929

Cited by 27 publications

(12 citation statements)

References 78 publications

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“…Second, amphibians are an excellent taxonomic group for eDNA‐based monitoring, as they abundantly release DNA into the water column via the excretion of mucus containing ample amounts of DNA (Adams et al, 2019). Third, while eDNA concentrations are generally quantified with quantitative PCR (qPCR), there are several indications that it underperforms in terms of resilience to PCR inhibitors (Doi et al, 2015), its accuracy (King et al, 2022), and its sensitivity (Brys, Halfmaerten, et al, 2021) in comparison with a ddPCR approach as was used here. Finally, the applied quantitative eDNA analyses were validated appropriately because bullfrog tadpole abundances were known exactly and eDNA concentrations were standardized among ponds on a total pond volume basis.…”

Section: Discussionmentioning

confidence: 99%

Using quantitative eDNA analyses to accurately estimate American bullfrog abundance and to evaluate management efficacy

et al. 2022

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Biological invasions contribute now more than ever to the global homogenization of fauna and flora. Large‐scale monitoring programs are, therefore, needed to detect incipient invasions and to evaluate management interventions. As conventional monitoring methods are constrained by large costs, environmental DNA (eDNA)‐based methods are increasingly recognized as valuable monitoring tools. However, accurately estimating species abundance from eDNA concentrations in natural systems remains challenging and consequently hinders their integration in management applications. Here, we used droplet digital PCR (ddPCR) in eDNA surveys to estimate the abundance of invasive American bullfrogs (Lithobates catesbeianus). We first introduced bullfrog tadpoles in natural ponds to assess the relationship between abundances and eDNA concentrations under field conditions. Next, we combined eDNA sampling with fyke netting in naturally colonized ponds to investigate whether bullfrog eDNA concentrations can estimate bullfrog capture success and conventional abundance measures obtained via depletion sampling. Finally, we evaluated eradication measures by comparing bullfrog eDNA concentrations before and after fyke netting. We found a strong linear relationship between the numbers of introduced tadpoles and eDNA concentrations (r2 = 0.988). Bullfrog eDNA concentrations were not only linearly related to the catch‐per‐unit‐effort (r2 = 0.739), but also to conventional abundance estimates (r2 = 0.716), particularly when eDNA concentrations were standardized for pond area (r2 = 0.834) and volume (r2 = 0.888). Bullfrog tadpoles were only captured when eDNA concentrations exceeded 1.5 copies µl−1, indicating that quantitative eDNA analyses enable the localization of breeding ponds. We found a significant reduction in eDNA concentrations after fyke netting proportional to the number of captured bullfrogs. These results demonstrate that eDNA quantification is a reliable tool that accurately estimates bullfrog abundance in natural lentic systems. We show that quantitative eDNA analyses can complement the toolbox of natural resource managers and facilitate the coordination of eradication campaigns targeting alien invasive species.

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Section: Discussionmentioning

confidence: 99%

Using quantitative eDNA analyses to accurately estimate American bullfrog abundance and to evaluate management efficacy

et al. 2022

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“…As a final remark, the technique employed in this study is able to detect NIS that are not always found from conventional surveys, as highlighted in other studies that used the same approach (Furlan et al, 2019;King et al, 2022). Despite current gaps in DNA reference databases (Weigand et al, 2019), eDNA metabarcoding could be used as a barometer of disturbance, helping to understand marine environments and complementing the existing survey methods (DiBattista et al, 2020).…”

Section: Discussionmentioning

confidence: 73%

Non-native species in the north Gulf of Aqaba (Red Sea) revealed from environmental DNA

Fernández

Ardura²,

Georges³

et al. 2022

Front. Mar. Sci.

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The movement of organisms facilitated by anthropogenic activities is a serious threat to marine diversity, especially for endemic species that may be outcompeted from non-indigenous species (NIS). In this study, we have analyzed communities inhabiting the north of the Gulf of Aqaba, Red Sea, employing environmental DNA (eDNA) metabarcoding. That gulf is especially rich in species and population endemism. We have detected NIS representing 36% of the total number of species found from eDNA. Primary producers were more abundant in the NIS than in the native fraction of species, suggesting that functional diversity could be altered if NIS thrive there. We discuss maritime traffic as a factor that may enhance the introduction of non-natives in this region and emphasize the importance of the control of these species that may threaten the rich endemic biota of the Red Sea.

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“…Although eDNA sampling for species presence is a relatively young field (Ficetola et al ., 2008) which has yet to settle on a standardized approach (Cristescu & Hebert, 2018), its many advantages over conventional methods and a better understanding of its error structure (Burian et al ., 2021) have led to its increasing acceptance for regional or national assessments of species distributions (Kasai et al ., 2021; King et al ., 2022; Matter et al ., 2018). We believe that many elements of this study can serve as a template for using model‐driven eDNA sampling as the basis for the high‐resolution, broad‐scale assessments of species of conservation concern.…”

Section: Discussionmentioning

confidence: 99%

Broad‐scale eDNA sampling for describing aquatic species distributions in running waters: Pacific lamprey Entosphenus tridentatus in the upper Snake River, USA

Young

Isaak

Nagel

et al. 2022

Journal of Fish Biology

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One of the most fundamental yet challenging tasks for aquatic ecologists is to precisely delineate the range of species, particularly those that are broadly distributed, require specialized sampling methods, and may be simultaneously declining and increasing in different portions of their range. An exemplar is the Pacific lamprey Entosphenus tridentatus, a jawless anadromous fish of conservation concern that is actively managed in many coastal basins in western North America. To efficiently determine its distribution across the accessible 56,168 km of the upper Snake River basin in the north-western United States, we first delimited potential habitat by using predictions from a species distribution model based on conventionally collected historical data and from the distribution of a potential surrogate, Chinook salmon Oncorhynchus tshawytscha, which yielded a potential habitat network of 10,615 km. Within this area, we conducted a two-stage environmental DNA survey involving 394 new samples and 187 archived samples collected by professional biologists and citizen scientists using a single, standardized method from 2015 to 2021. We estimated that Pacific lamprey occupied 1875 km of lotic habitat in this basin, of which 1444 km may have been influenced by recent translocation efforts. Pacific lamprey DNA was consistently present throughout most river main stems, although detections became weaker or less frequent in the largest and warmest downstream channels and near their headwater extent. Pacific lamprey were detected in nearly all stocked tributaries, but there was no evidence of indigenous populations in such habitats. There was evidence of post-stocking movement because detections were 1.8-36.0 km upstream from release sites. By crafting a model-driven spatial sampling template and executing an eDNA-based sampling campaign led by professionals and volunteers, supplemented by previously collected samples, we established a benchmark for understanding the current range of Pacific lamprey across a large portion of its range in the interior Columbia River basin. This approach could be tailored to refine range estimates for other wide-ranging aquatic species of conservation concern.

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Using eDNA to simultaneously detect the distribution of native and invasive crayfish within an entire country

Cited by 27 publications

References 78 publications

Using quantitative eDNA analyses to accurately estimate American bullfrog abundance and to evaluate management efficacy

Using quantitative eDNA analyses to accurately estimate American bullfrog abundance and to evaluate management efficacy

Non-native species in the north Gulf of Aqaba (Red Sea) revealed from environmental DNA

Broad‐scale eDNA sampling for describing aquatic species distributions in running waters: Pacific lamprey Entosphenus tridentatus in the upper Snake River, USA

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