Using DNA barcoding to differentiate invasive Dreissena species (Mollusca, Bivalvia)

Marescaux, Jonathan; Doninck, Karine Van

doi:10.3897/zookeys.365.5905

Cited by 20 publications

(10 citation statements)

References 22 publications

(38 reference statements)

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“…The colors in the figure follow the convention used by González et al (2015). Bootstrap support values were 100% for all nodes, with the exception of the few for which the actual value is shown in the picture Van Doninck, 2013) and lrn (DQ280038, KP052744, DQ333747: Giribet et al, 2006;Van der Velde et al, 2010) genes of D. polymorpha present in GenBank perfectly match the respective fragment of this genome, confirming its identity. BLAST searches with all annotated D. polymorpha mitochondrial genes against RefSeq database pointed at the mitogenome belonging to M. arenaria as the closest one.…”

Section: Mitogenome Datamentioning

confidence: 67%

Next-generation sequencing of Dreissena polymorpha transcriptome sheds light on its mitochondrial DNA

et al. 2017

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Zebra mussels Dreissena polymorpha (Veneroida, Dreissenidae) are known for their invasive behavior. Despite numerous studies dealing with this species, no results of large sequencing projects have been published to date, hampering marker development. In this study, we present a relatively large novel transcriptomic dataset obtained by Illumina MiSeq technology from mantle and male gonad of D. polymorpha sampled in Poland. The transcriptomic data were typical for the tissue analyzed. Moreover, they showed the expression of a single mitochondrial genome, indicating that this species do not have doubly uniparental inheritance of mitochondria. The sequences of mitochondrial transcripts were used to design primers and obtain nearly complete sequence of the zebra mussel mitochondrial genome by PCR and Sanger sequencing. This mitogenome has unique gene order, with the genes split into two blocks encoded in opposite directions. The closest mitogenome available in GenBank belongs to the marine clam Mya arenaria (Myoida, Myidae). The average divergence of the sequences of these mitogenomes is quite high, in the range of 20%. Both mitogenomic and transcriptomic resources should prove very useful for elucidating population genetics and conservation issues involving this important species.

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Section: Mitogenome Datamentioning

confidence: 67%

Next-generation sequencing of Dreissena polymorpha transcriptome sheds light on its mitochondrial DNA

et al. 2017

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“…The PCR-RFLP method is an assay that fulfills all these requirements. It is also a low-cost method which has the possibility of obtaining satisfactory results without the cost of sequencing the samples (Marescaux and Van Doninck, 2013).…”

Section: Resultsmentioning

confidence: 99%

“…New studies should also be conducted to assess the potential risks of these biological invasions (Pie et al, 2006a, Marescaux andVan Doninck, 2013), and reiterate that regardless of the choice, differents methods should be used for detection these mussels with the intention of getting more robust results in monitoring.…”

Section: Discussionmentioning

confidence: 99%

RFLP pattern determination for the invasive bivalves Limnoperna fortunei (Dunker, 1857) and Corbicula fluminea (Muller, 1774)

Oliveira¹,

Paula²,

Diniz³

et al. 2018

Rev. ambiente água

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The golden mussel (Limnoperna fortunei) and Corbicula fluminea are considered well-established invasive species in the rivers of Brazil and South America. In addition to the environmental problems resulting from this invasion process, the economic issue, especially in hydroelectric dams, is very worrisome and has mobilized several types of studies on these invasive bivalves. The detection and identification of these organisms in their adult phase in the rivers is not a problem; however, the identification of bivalve larvae by usual morphological methods is difficult due to high similarity conserved in these stages. The use of PCR-RFLP has proven to be an efficient and agile molecular method that allowed the detection of different patterns in the agarose gel for the two bivalves tested. The gel pattern showed a 100 bp band for L. fortunei not detected for C. fluminea. Thus, it is possible to detect larvae of these species from water samples, which can be a powerful tool for environmental monitoring programs on aquatic invasive species.

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“…In most of the comparative studies, the morphological criterion is chosen to identify the two species. However, the two species show high morphological similarities [97,98] that prevent from morphological consistent species identification [99]. Mussel's "angularity" has been proposed anyway to improve morphometric identification with discriminating thresholds for zebra and quagga mussels [100].…”

Section: The Usefulness Of Another Dreissenid Species D Rostriformimentioning

confidence: 99%

“…Mussel's "angularity" has been proposed anyway to improve morphometric identification with discriminating thresholds for zebra and quagga mussels [100]. Molecular genetic markers have then been developed to differentiate Dreissena species [97,98,101], but most of them are based on restriction fragment length polymorphism (RFLP) of cytochrome-C oxidase subunit I (COI gene). Such mitochondrial genetic tool does not allow identifying potential interspecific hybrids [97].…”

Section: The Usefulness Of Another Dreissenid Species D Rostriformimentioning

confidence: 99%

Mussel as a Tool to Define Continental Watershed Quality

Ladeiro¹,

Barjhoux²,

Bigot-Clivot³

et al. 2017

Organismal and Molecular Malacology

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Bivalves appear as relevant sentinel species in aquatic ecotoxicology and water quality assessment. This is particularly true in marine ecosystems. In fact, several biomonitoring frameworks in the world used mollusks since several decades on the base of contaminant accumulation (Mussel Watch, ROCCH) and/or biological responses called biomarker (OSPAR) measurements. In freshwater systems, zebra and quagga mussels could represent alternative sentinels, which could be seen as the counterparts of mussel marine species. This chapter presents original studies and projects underlying the interest of these freshwater mussels for water quality monitoring based on contaminant accumulation and biomarker development measurements. These sentinel species could be used as a tool for chemical/biological monitoring of biota under the European water framework directive and for the development of effect-based monitoring tools.

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Using DNA barcoding to differentiate invasive Dreissena species (Mollusca, Bivalvia)

Cited by 20 publications

References 22 publications

Next-generation sequencing of Dreissena polymorpha transcriptome sheds light on its mitochondrial DNA

Next-generation sequencing of Dreissena polymorpha transcriptome sheds light on its mitochondrial DNA

RFLP pattern determination for the invasive bivalves Limnoperna fortunei (Dunker, 1857) and Corbicula fluminea (Muller, 1774)

Mussel as a Tool to Define Continental Watershed Quality

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