Using Dna Barcode to Improve the Identification of Marine Fish Larvae, Case Study Coastal Water Near Jakarta and Banda Sea, Indonesia

Abstract

The sustainability of the exploitation of the Indonesian fishes depends heavily on many of fish's basic information including both larvae distribution and dispersal. However, the identification of fish larvae and juvenile to species is very limited. In this study, we employed DNA barcoding techniques to identify marine fish larvae to a species level in Jakarta Bay and Banda Sea by comparing the querries with sequences from adult stage as reference library to contribute on biodiversity information on that particular area. The result revealed that in Jakarta Bay, the molecular marker of a 471 bp region of the mitchondrial cyctochrome c oxidase I gene (COI) has been successfully found to be species-specific, genetic distance within species (0.0 -1.30 percent). There are total of 8 families, 5 genera and 5 species from a total 15 successful PCR that could be used to calculate the accuracy of larval fish identification in three taxonomic categories. Meanwhile in Banda Sea, for the adult specimens, after some of PCR experiment, we have successfully amplified 27 individuals, only 8 sequences available. There are a total 326 eggs and larvae which been collected from 19 stations, of the 28 successfully amplified PCR samples, 11 sequences were available for DNA analysis and at least 10 species used Banda Sea and surrounding area as their spawning ground. We prove the ability of COI barcodes to identify species level resolution from query sequences and to classify species from distinct geographical origins and determine of how the data retrieved give important information for proposing plans for conserving and managing of fisheries in the sea waters.

Temporal variation in abundance of bully sleepers (Pisces: Eleotridae) larvae and juveniles in Cisolok Estuary, Palabuhanratu Bay, Indonesia

“…This process can keep the fish specimens intact for an extended period. On the other hand, sample preservation with 96% ethanol was applied for DNA analysis [13].…”

The Moroccan Ichtyoplankton - A General Overview

“…In general the visual identification of ichthyoplankton is a difficult task due to the small size of eggs and larvae, it is done under a binocular lens which is mainly based on morphological criteria [22], however in some cases it is problematic, particularly for species of proximate size diameter [32], The molecular tool allows the uncertainties of the classical technique to be unveiled, specific genetic markers are often used [33,34],sample fixation plays a key role in the efficiency of DNA extraction [35].…”

DNA barcoding of commercially important reef fishes in Weh Island, Aceh, Indonesia

“…However, there is no record of reef fish species identification through DNA barcoding in Aceh. Related studies in the Indonesian waters have been coincidental as part of larger projects over a wider coverage, with limited representative specimens from the Aceh region (Anzani et al, 2019;Madduppa et al, 2017;Madduppa et al, 2016;Prehadi et al, 2015;Sembiring et al, 2015;Wardiatno et al, 2015;Wibowo et al, 2018). Thus, this investigation is the first comprehensive study of DNA barcoding of commercially important reef fishes in Indonesia, with focus on the marine waters of Weh Island.…”