Using cytogenetic analysis RAPD in determination of genetic variations among four species of ornamental fishes of family: Poecilidae (Order: Cyprinodontiform)

Abstract

The karyological and molecular analysis of four fresh water fish species of Family: Poecilidae and their genetic relationships have been studied. Xiphophorus maculates and Xiphophorus hellerii have the same diploid chromosome number 2n=48, but they were different in their karyotypes. Poecilia sphenops and Poecilia reticulata have the same diploid chromosome number 2n=46 and the same fundamental number FN=46, also the same karyotype one group of acrocentric chromosomes. Nine RAPD primers, showed monomorphic bands, were used for the construction of the dendrogram and a similarity matrix. A total of 65 bands were obtained; 39 of them were monomorphic bands. Similarity values among the studied samples ranged from 21% to 38%. High similarity value was obtained between Xiphophorus maculates and Xiphophorus hellerii. (38%) and the low similarity values were obtained between Xiphophorus hellerii and Poecilia reticulata (21%). The cluster analysis clearly differentiated Xiphophorus maculates and Xiphophorus hellerii from Poecilia sphenops and Poecilia reticulata. RAPD analysis confirmed that the four species under study are genetically different from each other. These cytogenetic data obtained can be applied for further studies in cytotaxonomy and evolutionary relationships of fishes.

Genetic Investigation of Some Perciformes Fish Species Using Karyological Analysis

“…Several authors including (Santini et al, 2009;Sato and Nishida, 2010;Kai et al, 2011;Mazzuchelli et al, 2012;Guyomard et al, 2012;Schartl et al, 2013;Eschmeyer and Fong, 2014, Grassi, et al, 2017, Abu Almaaty et al, 2015& 2017aAlmeida, et al, 2017; reported that chromosome numbers confirm a large diversity in species of fishes in Class Actinopterygii which exhibit wide range , ranging from 2n=16 in ( Sphaerichthys osphromenoides ) to 2n=134 (Corydoras aeneus).…”

“…The cytogenetic studies indicated that the diploid chromosome numbers of Actinopterygii ranging from 2n=16 to 2n =134 (Santini et al, 2009;Sato and Nishida, 2010;Kai et al, 2011;Mazzuchelli et al, 2012); Guyomard et al, 2012;Schartl et al, 2013;Eschmeyer and Fong, 2014;Grassi et al, 2017, Abu Almaaty et al, 2015& 2017aAlmeida et al, 2017).…”

Chromosomal Formula of Four Marine Fish Species from The Mediterranean Sea in Egypt

“…Most studies concluded that the diploid chromosome numbers of fishes ranging from 2n=16 to 2n =134. (Grassi et al, 2017, Abu Almaaty et al, 2015, 2017aAlmeida et al, 2017).…”