Using curcumin to prevent structural impairments of testicles in rats induced by sodium metabisulfite

Mahmoudi, Reza; Honarmand, Zahra; Karbalay‐Doust, Saied; Jafari-Barmak, Mehrzad; Nikseresht, Mohsen; Noorafshan, Ali

doi:10.17179/excli2017-143

Cited by 8 publications

(3 citation statements)

References 22 publications

(44 reference statements)

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“…In vitro analyses on Leydig cell lines have indicated that curcumin exerts a protective effect against a variety of stressors mainly by suppressing apoptosis and also alleviating oxidative stress and endoplasmic reticulum stress ( Chen et al 2019 , 2020 , Yildizbayrak & Erkan 2019 ). When assessed in vivo , curcumin’s protective role translates into preserved testicular volume, tubular epithelium height, tubule length, and number of viable Leydig and germ cells ( Noorafshan et al 2011 , Lin et al 2015 , Mahmoudi et al 2017 , Chen et al 2019 , Khosravi et al 2020 , Afshar et al 2021 ). In the present report, we show that curcumin reduces basal and H 2 O 2 -stimulated ROS levels in MA-10 Leydig cells at low concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…; Yildizbayrak & Erkan 2019). When assessed in vivo, curcumin's protective role translates into preserved testicular volume, tubular epithelium height, tubule length, and number of viable Leydig and germ cells(Noorafshan et al 2011;Lin et al 2015;Mahmoudi et al 2017;Chen et al 2019;Khosravi et al 2020;Afshar et al proposed as a modulator of the Nrf2 pathway in the testis, a transcription factor that regulates the expression of several antioxidant enzymes(Ashrafizadeh et al 2020). An in vivo study in rats has shown that…”

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Curcumin effects on Leydig cell functions and potential therapeutic uses

et al. 2023

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Curcumin has been ascribed with countless therapeutic effects, but its impact on testicular function has been scarcely researched. Leydig cells comprise the androgen-secreting population of the testis and may give rise to Leydig cell tumours (LCT). Due to their steroid-secreting nature, LCT entail endocrine, reproductive, and psychological disorders. Approximately 10% are malignant and do not respond to chemotherapy and radiotherapy. The aim of this study was to assess curcumin’s impact on Leydig cells functions and its potential effect on LCT growth. In vitro assays on MA-10 Leydig cells showed that curcumin (20 to 80 µmol/L) stimulates acute steroidogenesis, both in the presence and absence of db-cAMP. This effect is accompanied by an increase in StAR expression. Regarding curcumin’s in vitro cytostatic capacity, we show that 40 to 80 µmol/L curcumin reduces MA-10 Leydig cells’ proliferative capacity, which could be explained by the arrest in G2/M and the reduced viability due to the activation of the apoptotic pathway. Finally, CB6F1 mice were inoculated with MA-10 cells to generate ectopic LCT in both flanks. They received intraperitoneal injections of 20 mg/kg curcumin or vehicle every other day for 15 days. We unveiled curcumin’s capacity to inhibit LCT growth as evidenced by reduced tumour volume, weight, and area under the growth curves. No detrimental effects on general health parameters or testicular integrity were observed. These results provide novel evidence on curcumin’s effects on the endocrine cell population of the testis and propose this natural compound as a therapeutic agent for LCT.

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Section: Discussionmentioning

confidence: 99%

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Curcumin effects on Leydig cell functions and potential therapeutic uses

et al. 2023

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“…Similarly, in rat testes exposure to 7 and 70 mg/kg b.w. doses of SMBS causes loss of type A and B spermatogonia, spermatids, Sertoli, and Leydig cells along with reduction of seminiferous tubules and tubular epithelium (Mahmoudi et al, 2017).…”

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confidence: 96%

Adverse physiologic effects of a common food additive potassium metabisulfite (E224) in laboratory rats

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Khadija

et al. 2022

Food Processing Preservation

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Food additives prevent microbial growth, prolong shelf life, and give desired taste, color, and texture. Of several food additives, the use of sulfites in the wine industry dates to ancient Greeks and Romans.Sulfiting agents such as potassium metabisulfite, sodium metabisulfite, calcium or ammonium sulfite and bisulfites, the active component of which is sulfur dioxide, are used to preserve soft drinks, wine, beer, frozen foods, jams, jellies, fresh and dried fruits, vegetables, and as anti-browning agents. Cosmetics and pharmaceutical industries also use sulfites for various purposes (Häberle et al., 2017;

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