Using coupled monolithic rods for ultra-high peak capacity LC and LC–MS under normal LC operating pressures

Bones, Jonathan; Duffy, Cepta; Macka, Mirek; Paull, Brett

doi:10.1039/b714807k

Cited by 10 publications

(2 citation statements)

References 22 publications

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“…Lestremau et al generated 162,000 plates by coupling eight 25 cm 9 4.6 mm 9 5 lm ODS columns to separate seven compounds in *100 min [13]). Coupling of monolithic columns has also been reported [18][19][20]. In this approach, a large number of columns (up to fourteen) were required to achieve high numbers of theoretical plates.…”

Section: Introductionmentioning

confidence: 94%

High-Efficiency Liquid Chromatography Using Sub-2 μm Columns at Elevated Temperature for the Analysis of Sulfonamides in Wastewater

Shaaban

Górecki

2011

Chromatographia

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Efficiency in HPLC can be enhanced by increasing the column length and/or decreasing the particle size. The use of high temperature in HPLC has emerged as a valuable tool to overcome the increase in column backpressure when using small packing particles, as it allows for reduction in mobile phase viscosity. In this study, high plate count was obtained by coupling sub-2 lm columns at elevated temperature to reduce the viscosity of the mobile phase, thus reducing the column backpressure. At 80°C, up to three columns of 15 cm 9 4.6 mm I.D. packed with 1.8 lm particles could be coupled generating *84,000 theoretical plates for the last eluting compound. The number of theoretical plates was increased on average by a factor of *3.6 when three columns were coupled at 80°C compared with one column at 30°C. The relationships between separation efficiency and column length were examined using Van Deemter plots constructed at 30°C and 80°C for different column lengths. The advantages of using coupled columns in combination with elevated temperature for the environmental analysis were illustrated using test mixtures comprised of eight sulfonamides separated on one column at 30°C and three coupled columns at 80°C by isocratic elution. Sample clean up was carried out by employing solid-phase extraction (SPE) using Oasis HLB cartridges. The method developed was validated based on parameters such as linearity, precision, accuracy, detection and quantification limits. Recoveries generally ranged from 71.7 to 99% (with the exception of sulfanilamide), with standard deviations not higher than 4.7%. The detection limits of the method ranged from 0.6-2 lg L -1 , while limits of quantification were in the range 2-6.7 lg L -1 with UV detection.

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Section: Introductionmentioning

confidence: 94%

High-Efficiency Liquid Chromatography Using Sub-2 μm Columns at Elevated Temperature for the Analysis of Sulfonamides in Wastewater

Shaaban

Górecki

2011

Chromatographia

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“…However, the column length needs to be around 1.7 m, so 17 of the commercially available 100-mm columns length would need to be coupled in series. Although some authors have coupled up to 10 columns, the cost becomes rapidly prohibitive [94]. Despite the elevated maximum efficiency observed with monoliths, this approach is not competitive with HTLC, because the lower maximum backpressure capability (ΔP max =200 bar) limits the monolith length that can be employed.…”

Section: Evaluation Of Existing Approachesmentioning

confidence: 99%

New trends in fast and high-resolution liquid chromatography: a critical comparison of existing approaches

et al. 2009

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Recent developments in chromatographic supports and instrumentation for liquid chromatography (LC) are enabling rapid and highly efficient separations. Various analytical strategies have been proposed, for example the use of silica-based monolithic supports, elevated mobile phase temperatures, and columns packed with sub-3 microm superficially porous particles (fused core) or with sub-2 microm porous particles for use in ultra-high-pressure LC (UHPLC). The purpose of this review is to describe and compare these approaches in terms of throughput and resolving power, using kinetic data gathered for compounds with molecular weights ranging between 200 and 1300 g mol(-1) in isocratic and gradient modes. This study demonstrates that the best analytical strategy should be selected on the basis of the analytical problem (e.g., isocratic vs. gradient, throughput vs. efficiency) and the properties of the analyte. UHPLC and fused-core technologies are quite promising for small-molecular-weight compounds, but increasing the mobile phase temperature is useful for larger molecules, for example peptides.

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Current literature in mass spectrometry

2008

J. Mass Spectrom.

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INSTRUMENTAL TECHNIQUES & METHODSAguilera JOURNAL OF MASS SPECTROMETRY J. Mass Spectrom. 2008; 43: 1009-1020 Published online in Wiley InterScience (www.interscience.wiley.com). DOI:10.1002DOI:10. /jms.1304 Current literature in mass spectrometry (4 weeks journals -Search completed at 23rd. Apr. 2008) In order to keep subscribers up-to-date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of mass spectrometry. Each bibliography is divided into 11 sections: 1 Reviews; 2 Instrumental Techniques & Methods; 3 Gas Phase Ion Chemistry; 4 Biology/Biochemistry: Amino Acids, Peptides & Proteins; Carbohydrates; Lipids; Nucleic Acids; 5 Pharmacology/Toxicology; 6 Natural Products; 7 Analysis of Organic Compounds; 8 Analysis of Inorganics/Organometallics; 9 Surface Analysis; 10 Environmental Analysis; 11 Elemental Analysis. Within each section, articles are listed in alphabetical order with respect to author. GAS PHASE ION CHEMISTRY

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Using coupled monolithic rods for ultra-high peak capacity LC and LC–MS under normal LC operating pressures

Cited by 10 publications

References 22 publications

High-Efficiency Liquid Chromatography Using Sub-2 μm Columns at Elevated Temperature for the Analysis of Sulfonamides in Wastewater

High-Efficiency Liquid Chromatography Using Sub-2 μm Columns at Elevated Temperature for the Analysis of Sulfonamides in Wastewater

New trends in fast and high-resolution liquid chromatography: a critical comparison of existing approaches

Current literature in mass spectrometry

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