Using contemporary liquid chromatography theory and technology to improve capillary gradient ion-exchange separations

Wouters, Bert; Broeckhoven, Ken; Wouters, Sam; Bruggink, Cees; Agroskin, Yury; Pohl, Christopher A.; Eeltink, Sebastiaan

doi:10.1016/j.chroma.2014.10.028

Cited by 6 publications

(1 citation statement)

References 32 publications

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“…a [OH ‐ ] or pH gradient), in which case the salt gradient should follow the same rules as the organic modifier gradient in RPLC, i.e. the components should experience the same history of conditions [39,62–66]. For hydrophobic interaction chromatography (HIC), a similar situation arises, but here an inverse salt gradient is applied [39], where both the salt gradient time/column void time and range of the salt gradient (high initial and low end concentration) should remain the same for all flow rates and column lengths.…”

Section: Kinetic Plots In Lc (Negligible Mobile Phase Compressibility)mentioning

confidence: 99%

Methods to determine the kinetic performance limit of contemporary chromatographic techniques

Broeckhoven

Desmet

2020

J of Separation Science

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By combining separation efficiency data as a function of flow rate with the column permeability, the kinetic plot method allows to determine the limits of separation power (time vs. efficiency) of different chromatographic techniques and methods. The technique can be applied for all different types of chromatography (liquid, gas, or supercritical fluid), for different types of column morphologies (packed beds, monoliths, open tubular, micromachined columns), for pressure and electro‐driven separations and in both isocratic and gradient elution mode. The present contribution gives an overview of the methods and calculations required to correctly determine these kinetic performance limits and their underlying limitations.

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Section: Kinetic Plots In Lc (Negligible Mobile Phase Compressibility)mentioning

confidence: 99%