Using complex coacervation for lysozyme encapsulation by spray-drying

Preparation of a balsa-lysozyme eco-friendly dressing and its effect on wound healing

“…Additionally, lysozyme contains four 2-disulde bonds, consists of two regions connected by a long alpha helix, and has mostly alpha helical secondary structure. 9,10 As shown in Fig. 3, there are two typical two characteristic peaks of lysozyme, 11 but the intensity is decreased.…”

“…The higher the content of lysozyme, the more likely it is to be coated on the surface of balsa, which results in less content of lysozyme and low encapsulation efficiency. 7,9,10 However, the encapsulation efficiency of lysozyme at different concentrations was about 60%, suggesting this method was effective for lysozyme embedding. The release rates of B, C, D and E groups tended to be stable and highest at 48 h, with cumulative release percentages of 80.7%, 90.6%, 91.4% and 92.3% aer 72 h. As for the release of drugs, lysozyme released faster in the rst 24 h, suggesting early release may be possible.…”

“…9 In recent years, there have been many studies to prepare lysozyme into microcapsules, nanoparticles, and pellets, and combine with different materials to improve its stability and its Gram-negative bacteria bactericidal effect. 7,10,11 Some studies have pointed out the hydrolysis activity of PTFE membrane loaded with lysozyme against the micrococcus lysate can reach 3.5 U cm À2 , which has perfect antibacterial activity against the important pathogenic Staphylococcus aureus, and the antibacterial rate reaches 89%. The antibacterial rate is expected to be further improved if the rate of enzyme load per unit area is increased.…”

“…Clinically, it is oen used in the form of a microballoon or in combination with other antimicrobial agents. 10,11 For patients with severe skin defects, there is an urgent need to quickly and effectively close the wound. Effective and inexpensive strategies to prevent bacterial invasion and the loss of body uids are needed.…”

Designing biopolymer microgels to encapsulate, protect and deliver bioactive components: Physicochemical aspects

“…The stability of electrostatic complexes to dissociation can often be improved after they have been formed by carrying out an additional internal cross-linking step or by coating them with biopolymer shells [87]. Internal cross-linking of the biopolymers may be carried out [88][89][90], nutraceuticals (e.g., anthocyanins, astaxanthin, -carotene, and curcumin) [91][92][93][94], proteins and peptides [95,96], lipids [97,98], and flavors [99][100][101].…”