Using automated patch clamp electrophysiology platforms in ion channel drug discovery: an industry perspective

Rogers, Marc; Obergrussberger, Alison; Kondratskyi, Artem; Fertig, Niels

doi:10.1080/17460441.2024.2329104

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article2

Preprint1

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

References 103 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

From viruses to humans – Exploring the structure–function relationship of the Kesv protein for the future of biomedicine

Asrani,

Seebohm,

Stoll

2024

Journal of Structural Biology

View full text Add to dashboard Cite

From viruses to humans – Exploring the structure–function relationship of the Kesv protein for the future of biomedicine

Asrani,

Seebohm,

Stoll

2024

Journal of Structural Biology

View full text Add to dashboard Cite

Machine learning and deep learning approaches for enhanced prediction of hERG blockade: a comprehensive QSAR modeling study

Liu,

Khan,

Guo

et al. 2024

Expert Opinion on Drug Metabolism & Toxicology

View full text Add to dashboard Cite

Transcriptomic analysis and high throughput functional characterization of human induced pluripotent stem cell derived sensory neurons

Truong,

Brougher,

Strassmaier

et al. 2024

Preprint

View full text Add to dashboard Cite

Peripheral sensory neurons are a primary effector in pain neurotransmission, and have become a useful cellular model for the study of pain. While rodent tissue has historically served as a source of these neurons, it has become increasingly clear that pain mechanisms in rodents and humans are substantially divergent. Sensory neurons harvested from cadaveric human tissue serve as a superior translational model for studying pain mechanisms, however their relative paucity limits their widespread utility. Theoretically, sensory neurons manufactured from human pluripotent stem cells (hPSCs) could help bridge this translational gap given their relative abundance and potential similarity to primary human tissue. However, hPSC-derived sensory neurons manufactured with the most common methodologies correlate poorly to human tissue both transcriptionally and functionally. In the present work, we compare a novel population of hPSC-derived sensory neurons to previously published datasets and find this novel population to more closely resemble human primary dorsal root ganglia transcriptionally. Furthermore, we evaluate the heterogeneity of this novel population via single nucleus RNA sequencing and find it resembles specific nociceptor and mechanoreceptor subsets found in vivo. Finally, we assay the functionality of this population with high throughput automated patch clamp electrophysiology with respect to voltage-gated sodium (Nav) and potassium channels (Kv), and ligand-gated ionotropic GABA and P2X receptors. Overall, we find this population of hPSC-derived sensory neurons to be of relatively high fidelity, and suitable for interrogating numerous potential pain targets on a fully humanized platform.

show abstract

Using automated patch clamp electrophysiology platforms in ion channel drug discovery: an industry perspective

Cited by 3 publications

References 103 publications

From viruses to humans – Exploring the structure–function relationship of the Kesv protein for the future of biomedicine

From viruses to humans – Exploring the structure–function relationship of the Kesv protein for the future of biomedicine

Machine learning and deep learning approaches for enhanced prediction of hERG blockade: a comprehensive QSAR modeling study

Transcriptomic analysis and high throughput functional characterization of human induced pluripotent stem cell derived sensory neurons

Contact Info

Product

Resources

About