Using Advanced Correlative Microscopy to Study Complex Biological Samples

Meo, Josey Sueters Di; Liv, Nalan; Hoogenboom, Jacob P.

doi:10.1002/9780470027318.a9473

Cited by 3 publications

(2 citation statements)

References 193 publications

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“…Raman scattering is based on inelastic light scattering and changes in molecular polarizability, allowing even normal IR-inactive vibrations to produce signals, due to their fundamental molecular vibrations [ 183 ]. Surface-enhanced techniques are the most sensitive way to use Raman scattering, allowing the analysis of complex functional and structural characteristics of single biomolecules [ 184 ].…”

Section: Spectroscopic Monitoring Techniquesmentioning

confidence: 99%

Use of Spectroscopic Techniques to Monitor Changes in Food Quality during Application of Natural Preservatives: A Review

et al. 2020

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Consumer demand for food of high quality has driven research for alternative methods of food preservation on the one hand, and the development of new and rapid quality assessment techniques on the other hand. Recently, there has been a growing need and interest in healthier food products, which has led to an increased interest in natural preservatives, such as essential oils, plant extracts, and edible films and coatings. Several studies have shown the potential of using biopreservation, natural antimicrobials, and antioxidant agents in place of other processing and preservation techniques (e.g., thermal and non-thermal treatments, freezing, or synthetic chemicals). Changes in food quality induced by the application of natural preservatives have been commonly evaluated using a range of traditional methods, including microbiology, sensory, and physicochemical measurements. Several spectroscopic techniques have been proposed as promising alternatives to the traditional time-consuming and destructive methods. This review will provide an overview of recent studies and highlight the potential of spectroscopic techniques to evaluate quality changes in food products following the application of natural preservatives.

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Section: Spectroscopic Monitoring Techniquesmentioning

confidence: 99%

Use of Spectroscopic Techniques to Monitor Changes in Food Quality during Application of Natural Preservatives: A Review

et al. 2020

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“…Correlative light and electron microscopy (CLEM) helps to bridge this gap between LM and EM of GV-infected cells with the combination of the specificity of fluorescent labeling and the high-resolution structural information of EM, making it the perfect tool to study the complex relationship between form and function in biology. The SECOM system is a system for integrated CLEM wherein light and electron imaging are performed in one system without the need for sample transfer [16] , [17] .…”

Section: Introductionmentioning

confidence: 99%

Correlative light electron microscopy of giant viruses with the SECOM system

Baudoin

Hari²,

Sahmi-Bounsiar

et al. 2018

New Microbes and New Infections

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Bright-field transmission electron microscopy (TEM), TEM-negative staining technique, resin-embedding and ultramicrotomy, scanning TEM, scanning electron microscopy, atomic force microscopy and cryoelectron microscopy are imaging techniques used for describing giant viruses, their cycle and ultrastructure. Here we used the SECOM system, an integrated correlative light and electron microscopy using light and electronic imaging without sample transfer, to study cells infected with giant viruses, as shown by Tupanvirus, the ultrastructure of which was successfully observed. An improvement of the SECOM system with an eye to its use in fundamental and clinical research could be considered in the field of microbiology.

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Improving 3d correlation in integrated correlated light and electron microscopy using confocal laser scanning microscopy

Meo

Kruit

Hoogenboom

2016

European Microscopy Congress 2016: Proceedings

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In CLEM experiments biological samples are typically first investigated by light microscopy (LM) to locate a region of interest. Next, nanometer‐scale imaging can be achieved by electron microscopy (EM). This results in identification of biomolecules within the ultrastructure and/or the possibility to correlate ultrastructure with preliminary live‐cell dynamics on biomolecules [1]. We have developed an integrated CLEM with which widefield fluorescence microscopy (WF‐FM) and EM can be performed in principle simultaneously [2]. Integration of these two modalities allows for fast ROI retrieval [3], optional fast switching between modalities increasing CLEM throughput, high (5 nm) correlation accuracy for the overlay [4], and novel imaging applications [5]. However, for WF‐FM the depth correlation for thick specimen or live cells contained in a dedicated liquids sample holder [6] is relatively poor. WF‐FM images through the entire sample and SEM only the upper ~100 nm (depending on e‐beam energy). We therefore integrated a Confocal Laser Scanning Microscope (CLSM) into a scanning electron microscope (SEM) via a Delmic Secom stage (Fig. 1‐4). This system will allow imaging with improved depth‐correlation by suppression of out‐of‐focus light contributions and will provide 3D optical information in CLEM. We present our integrated CLSM SEM, using a commercial confocal scanner unit. We also demonstrate the procedure for alignment of the CLSM and SEM axes, needed to image the same area with both modalities. Lastly, the results of proof‐of‐principle experiments are presented, showing optical sectioning in thick specimen such as resin blocks or whole cells for improved LM‐SEM correlation along the axial direction.

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Using Advanced Correlative Microscopy to Study Complex Biological Samples

Cited by 3 publications

References 193 publications

Use of Spectroscopic Techniques to Monitor Changes in Food Quality during Application of Natural Preservatives: A Review

Use of Spectroscopic Techniques to Monitor Changes in Food Quality during Application of Natural Preservatives: A Review

Correlative light electron microscopy of giant viruses with the SECOM system

Improving 3d correlation in integrated correlated light and electron microscopy using confocal laser scanning microscopy

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