Using a transcriptomic approach to understand poor growth performance in farmed orange-spotted grouper (
            <i>Epinephelus coioides</i>
            ) larvae: a case study in a commercial hatchery

Ng, S; Lau, Cher Chien; Tan, Min Pau; Nor, Siti Azizah Mohd; Danish-Daniel, Muhd; Afiqah‐Aleng, Nor; Muchlisin, Zainal A.; Fadli, Nur

doi:10.1080/00288330.2023.2230914

Cited by 2 publications

(1 citation statement)

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“…Merbok River has been known to host various aquaculture activities, including the farming of orangespotted groupers (Ng et al 2023) and oysters (Lim et al 1995). The river was classi ed as heavilypolluted since decades ago (Lim et al 1995), and the condition has not improved (likely worsened), a consequence of anthropogenic activities at the upper catchment and weathering (Ismail and Ibrahim 2015;Msahir Atshan et al 2020;Zulki i 2022).…”

Section: Introductionmentioning

confidence: 99%

The gut microbiome of healthy and moribund orange-spotted groupers reveals specialised metabolite profiles and potential beneficial species: A case study in Malaysian fish farm

Lau,

Nor,

Noor

et al. 2023

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The gut microbiome of carnivorous fish evolves in tandem with the host to adaptively increase the host fitness in a dynamically-changing environment. In order to identify the beneficial strains in supporting the survival of orange-spotted grouper, Epinephelus coioides in a culture environment, the foregut, midgut and hindgut were analysed in three healthy and two moribund individuals. This study was conducted in the Merbok River, northwest Peninsular Malaysia which is exposed to some degree of pollution. The 16S amplicon sequencing targeting the V3-V4 highly variable region revealed similar community richness among samples but compositional and functional differences between the healthy and moribund fishes in the three gut regions. The findings showed a higher abundance of beneficial microflora, including Halomonas, Curvibacter and Bacillus residing in healthy foreguts, coupled with elevated activities of aromatic compound degradation. In the midguts, more than 70% of microbiota comprised of Photobacterium and Vibrio in most specimens. The higher abundance of Vibrio and Photobacterim in moribund fish hindguts may be correlated with increased chitinolytic activity and altered metabolic pathways. The outcomes of the study provide a fundamental understanding of the gut microbiota structure and suggest the potential use of Halomonas, Curvibacter and Bacillus in the orange-spotted grouper survival in a polluted environment.

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