Using a Spectrofluorometer for Resonance Raman Spectra of Organic Molecules

Abstract

Scattering (Rayleigh and Raman) and fluorescence are two common light signals that frequently occur together, confusing the researchers and graduate students experimenting in molecular spectroscopy laboratories. This report is a brief study presenting a clear discrimination between the two signals mentioned, employing a common spectrofluorometer such as the PerkinElmer LS 55. Even better, the resonance Raman signal of a molecule (e.g., acetone) can be obtained elegantly using the same instrument.

Second-Order Scattering Quenching in Fluorescence Spectra of Natural Humates as a Tracer of Formation Stable Supramolecular System for the Delivery of Poorly Soluble Antiviral Drugs on the Example of Mangiferin and Favipiravir

“…It is a well-known fact that scattering is many times more common than fluorescence; however, if the molecule fluorescence is tremendously high it is almost impossible to observe the scattering signals. On the other hand, in the weakly fluorescent molecules, the scattering signals are comparable to, and sometimes even higher than, the fluorescence [59]. So, the tryptophan fluorescence expressed in the BSA molecule is quite intense but still allows us to see the second-order scattering signal at 500 nm.…”

Narrowband Spontaneous Emission Amplification from a Conjugated Oligomer Thin Film

“…A Perkin Elmer Lambda 950 spectrophotometer (Llantrisant, United Kingdom) was used to record the absorption spectra over a wide range (200-1000 nm). The fluorescence spectra were measured using a spectrofluorometer (LS 55, from the same company) over a scan range of 200 to 1000 mm [34]. The laser pulse (a 5 ns laser of 3rd harmonic (355 nm) from Nd:YAG) (Brilliant B, Quantel, Les Ulis, France) was focused by a cylindrical lens with a 5 cm focal length and fixed horizontally; therefore, the laser pulse was concentrated to a strip of 1 mm × 1 cm (length × width), as shown in Figure 2.…”

In situ surface film evolution during Mg aqueous corrosion in presence of selected carboxylates

“…5a). However, formation of a specific network of the organic molecules which contains a chain of alternating double and single bonds, called conjugation, or π-electron delocalization can cause fluorescence [79,80]. It was reported that PDC can form so-called coordination polymer with different dimensionality, consisting of the long chains of PDC molecules bonded due to the electrostatic interaction with metal ions [80][81][82].…”