Using a microdialysis shunt probe to monitor phenolphthalein glucuronide in rats with intact and diverted bile flow

Heppert, Kathleen E.; Davies, Malonne I.

doi:10.1016/s0003-2670(98)00597-2

Cited by 10 publications

(5 citation statements)

References 13 publications

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“…before probe implantation surgery, and they remained anesthetized throughout the experimental period. Implantation of the shunt microdialysis probe was accomplished through a midline incision in the abdominal wall [22]. Bile samples were drawn every 2 h after administration of notoginseng extract until 12 h. The rat’s body temperature was maintained at 37°C during the experimental procedure.…”

Section: Methodsmentioning

confidence: 99%

Analysis of Panax notoginseng metabolites in rat bile by liquid chromatography–quadrupole time-of-flight mass spectrometry with microdialysis sampling

Xiao

Yang

Hua

et al. 2012

Journal of Chromatography B

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A dynamic microdialysis sampling method with liquid chromatography-quadrupole time-of-flight mass spectrometry (Q-TOF-MS) was developed for rapid and sensitive analysis of the metabolite profile of Panax notoginseng extract (PNE) in rat bile. In vivo studies in male Sprague-Dawley rats were performed with microdialysis probes implanted into the bile duct before bile samples were collected from 0–12 h. Metabolites of PNE were identified using dynamic adjustment of the fragmentor voltage to produce structure-relevant fragment ions. The mass accuracy of precursor and fragment ions was typically within 5 ppm of the theoretical values. We identified 7 compounds: 4 parent compounds (notoginsenoside R1, ginsenosides Rg1, Rb1, and Rd) and 3 metabolites (ginsenosides Rg2, Rh2, and compound K). Data from this study suggest that this microdialysis technique could be used in notoginseng saponin metabolic animal studies.

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Section: Methodsmentioning

confidence: 99%

Analysis of Panax notoginseng metabolites in rat bile by liquid chromatography–quadrupole time-of-flight mass spectrometry with microdialysis sampling

Xiao

Yang

Hua

et al. 2012

Journal of Chromatography B

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“…Hadwiger et al [36] applied this probe type to study the tacrine and its metabolites pharmacokinetics in rat bile microdialysates. Heppert et al [37] using the microdialysis shunt probe to monitor phenolphthalein glucuronide in rats with intact and diverted bile flow during the enterohepatic circulation, EHC.…”

Section: The Shunt Probementioning

confidence: 99%

Crosstalk the Microdialysis in Scientific Research: from Principle to its Applications

Lee¹,

Di²

2013

Pharm Anal Acta

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“…A 10 μL sample in these fractions (B) was injected into the HPLC system. Recovery (%) at each fl ow rate was calculated by the following equation (1) (Heppert and Davies 1999). (1)…”

Section: In Vitro Recoverymentioning

confidence: 99%

Determination of kynurenic acid levels in rat brain microdialysis samples and changes in kynurenic acid levels induced by N‐acetylaspartic acid

Tomiya¹,

Fukushima²,

Ogaya³

et al. 2009

Biomedical Chromatography

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The levels of kynurenic acid, an endogenous antagonist of alpha(7) nicotinic acetylcholine and N-methyl-D-aspartate receptors, were measured in microdialysis samples obtained from the prefrontal cortices of rats using column-switching high-performance liquid chromatography with fluorescence detection. When the perfusate was constantly infused at a rate of 1.0 mu/min, the in vitro recovery of kynurenic acid through the dialysis membrane was approximately 20.4%, and the precision was within 1.31%. Endogenous kynurenic acid in the microdialysis sample was clearly detected using column-switching high-performance liquid chromatography. As an application study, N-acetyl-L-aspartic acid, an endogenous metabolite and precursor of N-acetyl-L-aspartyl-L-glutamic acid, which is an agonist of metabotropic glutamate receptors, was infused for 120 min through the microdialysis probe. The kynurenic acid level significantly increased during the infusion of N-acetyl-L-aspartic acid, suggesting that kynurenic acid might have some association with N-acetyl-L-aspartic acid in vivo.

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Using a microdialysis shunt probe to monitor phenolphthalein glucuronide in rats with intact and diverted bile flow

Cited by 10 publications

References 13 publications

Analysis of Panax notoginseng metabolites in rat bile by liquid chromatography–quadrupole time-of-flight mass spectrometry with microdialysis sampling

Analysis of Panax notoginseng metabolites in rat bile by liquid chromatography–quadrupole time-of-flight mass spectrometry with microdialysis sampling

Crosstalk the Microdialysis in Scientific Research: from Principle to its Applications

Determination of kynurenic acid levels in rat brain microdialysis samples and changes in kynurenic acid levels induced by N‐acetylaspartic acid

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