Using A Fiber Optic Particle Plasmon Resonance Biosensor To Determine Kinetic Constants of Antigen–Antibody Binding Reaction

Abstract

In this paper, one simple and label-free biosensing method has been developed for determining the binding kinetic constants of antiovalbumin antibody (anti-OVA) and anti-mouse IgG antibody using the fiber optic particle plasmon resonance (FOPPR) biosensor. The FOPPR sensor is based on gold-nanoparticle-modified optical fiber, where the gold nanoparticle surface has been modified by a mixed self-assembled monolayer for conjugation of a molecular probe reporter (ovalbumin or mouse IgG) to dock with the corresponding analyte species such as anti-OVA or anti-mouse IgG. The binding process, occurring when an analyte reacts with a probe molecule immobilized on the optical fiber, can be monitored in real-time. In addition, by assuming a Langmuir-type adsorption isotherm to measure the initial binding rate, the quantitative determination of binding kinetic constants, the association and dissociation rate constants, yields k(a) of (7.21 ± 0.4) × 10(3) M(-1) s(-1) and k(d) of (2.97 ± 0.1) × 10(-3) s(-1) for OVA/anti-OVA and k(a) of (1.45 ± 0.2) × 10(6) M(-1) s(-1) and k(d) of (2.97 ± 0.6) × 10(-2) s(-1) for mouse IgG/anti-mouse IgG. We demonstrate that the FOPPR biosensor can study real-time biomolecular interactions.

Effect of Surface Coverage of Gold Nanoparticles on the Refractive Index Sensitivity in Fiber-Optic Nanoplasmonic Sensing

“…Gold nanoparticles (AuNP) have been used over a wide range of fields in chemical and biochemical analyses such as clinical analysis [ 1 , 2 , 3 , 4 , 5 , 6 ], molecular diagnostics [ 7 , 8 ] bio-interaction analysis and drug discovery [ 9 ], agricultural diagnostics [ 10 , 11 ], environmental monitoring [ 12 ], and food safety analysis [ 13 , 14 , 15 ]. The energy density of an AuNP varies with particle size [ 16 ] and shape [ 17 , 18 ], leading to a special absorption band of AuNP known as the plasmon resonance (PR) band [ 19 ].…”

Electrochemical Immunosensor for Detection of Epidermal Growth Factor Reaching Lower Detection Limit: Toward Oxidized Glutathione as a More Efficient Blocking Reagent for the Antibody Functionalized Silver Nanoparticles and Antigen Interaction

“…In addition, nanomaterials that were used for amplifying signal have been widely reported in previous work. [30][31][32][33] In the present work, the signal amplifying effect of Ab-AgNPs was Table S1 in Supporting Information. [34][35][36][37] As far as we know, our method has the lowest detection limit and the widest linear range.…”

Fiber Optic Particle Plasmon Resonance Biosensor for Label-Free Detection of Nucleic Acids and Its Application to HLA-B27 mRNA Detection in Patients with Ankylosing Spondylitis

“…Since the absorption coefficient of AuNPs increases when the AuNP's surrounding local refractive index (RI) increases [12]. Hence, the increase of local RI at the AuNP surface during molecular binding can be revealed in real-time by a decrease of transmitted light intensity through the optical fiber in form of a molecular binding kinetic curve [29]. In this study, the sensor response is defined as I/I 0 , where I 0 is the light intensity exiting a sensor fiber which is immersed in a blank and I is the light intensity exiting the same sensor fiber which is immersed in a sample.…”