Using a Core–Sheath Distribution of Surface Chemistry through 3D Tissue Engineering Scaffolds to Control Cell Ingress

Barry, John J. A.; Howard, Daniel J.; Shakesheff, Kevin M.; Howdle, Steven M.; Alexander, Morgan R.

doi:10.1002/adma.200502719

Cited by 94 publications

(77 citation statements)

References 20 publications

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“…This observation can be attributed to the tendency of films, in some cases, to delaminate and break off into non-uniform pieces, thus exposing different amounts of surface area. As stated before, this technique is amenable to investigate drug/protein release from alternate geometries such as three-dimensional scaffolds used for tissue engineering [50][51][52] or core-shell particles used for multidrug therapies.…”

Section: Resultsmentioning

confidence: 99%

Novel, High Throughput Method to Study in Vitro Protein Release from Polymer Nanospheres

2009

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Controlled delivery of therapeutic protein drugs using biodegradable polymer carriers is a desired characteristic that enables effective, application-specific therapy and treatment. Previous studies have focused on protein delivery from polymers using conventional "one-sample-at-a-time" techniques, which are timeconsuming and costly. In addition, many therapeutic proteins are in limited supply and are expensive, so it is desirable to reduce sample size for design and development of delivery devices. We have developed a rapid, high throughput technique based on a highly sensitive fluorescence-based assay to detect and quantify protein released from polyanhydrides while utilizing relatively small amounts of protein (âˆ¼40 Î¼g). These studies focused on the release of a model protein, Texas Red conjugated bovine serum albumin, from polyanhydride copolymers based on sebacic acid (SA) and 1,6-bis(p-carboxyphenoxy)hexane (CPH). The protein release profiles were assessed simultaneously to investigate the effect of polymer device geometry (nanospheres vs films), polymer chemistry, and pH of the release medium. The results indicated that the nanosphere geometry, SA-rich chemistries, and neutral pH release medium led to a more rapid release of the protein compared to the film geometry, CPH-rich chemistries, and acidic pH release medium, respectively. This high throughput fluorescence-based method can be readily extended to study release kinetics for other proteins and polymer systems. KeywordsBovine serum albumin, drug carrier, fluorescent dye, nanosphere, plasma protein, polymer, combinatorial chemistry, cattle Controlled delivery of therapeutic protein drugs using biodegradable polymer carriers is a desired characteristic that enables effective, application-specific therapy and treatment. Previous studies have focused on protein delivery from polymers using conventional "one-sample-at-a-time" techniques, which are time-consuming and costly. In addition, many therapeutic proteins are in limited supply and are expensive, so it is desirable to reduce sample size for design and development of delivery devices. We have developed a rapid, high throughput technique based on a highly sensitive fluorescence-based assay to detect and quantify protein released from polyanhydrides while utilizing relatively small amounts of protein (∼40 µg). These studies focused on the release of a model protein, Texas Red conjugated bovine serum albumin, from polyanhydride copolymers based on sebacic acid (SA) and 1,6-bis(p-carboxyphenoxy)hexane (CPH). The protein release profiles were assessed simultaneously to investigate the effect of polymer device geometry (nanospheres vs films), polymer chemistry, and pH of the release medium. The results indicated that the nanosphere geometry, SA-rich chemistries, and neutral pH release medium led to a more rapid release of the protein compared to the film geometry, CPH-rich chemistries, and acidic pH release medium, respectively. This high throughput fluorescence-based method can be readily exten...

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Section: Resultsmentioning

confidence: 99%

Novel, High Throughput Method to Study in Vitro Protein Release from Polymer Nanospheres

2009

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“…Such surface-modifi ed scaffolds were shown to improve fi broblast attachment and proliferation in vitro . Plasma polymerization, another plasma-based surface modifi cation approach, is distinct from plasma grafting by coating the substrate instead of covalent binding species to a plasma-treated polymer surface (Barry et al , 2006 ;Morent et al , 2011 ). Under plasma conditions, gaseous or liquid monomers are converted into reactive fragments which can, in turn, recombine to form a polymer fi lm which is deposited onto the substrate exposed to the plasma.…”

Section: Plasma-based Surface Modifi Cationmentioning

confidence: 99%

Fabrication of nanofibrous scaffolds for tissue engineering applications

Chen

Truckenmüller

Blitterswijk

et al. 2013

Nanomaterials in Tissue Engineering

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Nanofi brous scaffolds which mimic the structural features of a natural extracellular matrix (ECM) can be appealing scaffold candidates for tissue engineering as they provide similar physical cues to the native environment of the targeted tissue to regenerate. This chapter discusses different strategies to fabricate nanofi brous scaffolds for tissue engineering. We fi rst describe three major methods for nanofi brous scaffold fabrication: molecular self-assembly, phase separation, and electrospinning. Then, approaches for surface modifi cation of nanofi brous scaffolds including blending and coating, plasma treatment, wet chemical methods, and surface graft polymerization are presented. Finally, applications of nanofi brous scaffolds in tissue engineering are introduced.

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“…Bulk properties of the polymer are not altered through the grafting of high-density chains, in a precise location on the polymer surface. In addition, the chemical bond formed between the polymer surface and the grafted functional group results in a more durable functionalization in comparison with the physical coating [168,169]. Plasma grafting is restricted to localized surface areas and to a depth from several hundred angstroms to 10 nm [154].…”

Section: Plasma Grafting and Plasma Treatmentmentioning

confidence: 99%

Strategies for the chemical and biological functionalization of scaffolds for cardiac tissue engineering: a review

Tallawi

Rosellini

Barbani

et al. 2015

J. R. Soc. Interface.

282

208

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The development of biomaterials for cardiac tissue engineering (CTE) is challenging, primarily owing to the requirement of achieving a surface with favourable characteristics that enhances cell attachment and maturation. The biomaterial surface plays a crucial role as it forms the interface between the scaffold (or cardiac patch) and the cells. In the field of CTE, synthetic polymers ( polyglycerol sebacate, polyethylene glycol, polyglycolic acid, poly-L-lactide, polyvinyl alcohol, polycaprolactone, polyurethanes and poly(N-isopropylacrylamide)) have been proven to exhibit suitable biodegradable and mechanical properties. Despite the fact that they show the required biocompatible behaviour, most synthetic polymers exhibit poor cell attachment capability. These synthetic polymers are mostly hydrophobic and lack cell recognition sites, limiting their application. Therefore, biofunctionalization of these biomaterials to enhance cell attachment and cell material interaction is being widely investigated. There are numerous approaches for functionalizing a material, which can be classified as mechanical, physical, chemical and biological. In this review, recent studies reported in the literature to functionalize scaffolds in the context of CTE, are discussed. Surface, morphological, chemical and biological modifications are introduced and the results of novel promising strategies and techniques are discussed.

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Using a Core–Sheath Distribution of Surface Chemistry through 3D Tissue Engineering Scaffolds to Control Cell Ingress

Cited by 94 publications

References 20 publications

Novel, High Throughput Method to Study in Vitro Protein Release from Polymer Nanospheres

Novel, High Throughput Method to Study in Vitro Protein Release from Polymer Nanospheres

Fabrication of nanofibrous scaffolds for tissue engineering applications

Strategies for the chemical and biological functionalization of scaffolds for cardiac tissue engineering: a review

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