Using a biomimetic membrane surface experiment to investigate the activity of the magnetite biomineralisation protein Mms6

Bird, Scott M.; Rawlings, Andrea E.; Galloway, Johanna M.; Staniland, Sarah S.

doi:10.1039/c5ra16469a

Cited by 34 publications

(50 citation statements)

References 35 publications

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“…In contrast, FliC‐Mms6_C subunits were not able to interact with the magnetite surface. These results are consistent with a recent study that suggested that Mms6 binds to iron ions (especially ferrous) rather than to magnetite surfaces . The same study found that the full protein—not just the C‐terminal section—is required for the iron‐binding property of Mms6.…”

Section: Discussionsupporting

confidence: 92%

“…Recently it was demonstrated that Mms6 binds to ferric and ferrous ions rather than to magnetite surfaces, and is a nucleating agent rather than a controller of magnetite crystal growth . Mms6 attached to surfaces has been used for the patterned formation of magnetite . In our experiments, the loop region of MamI and the 25‐residue C‐terminal region of Mms6 were inserted into flagellin in place of the D3 domain.…”

Section: Introductionmentioning

confidence: 87%

“…The 25‐residue C‐terminal region was suggested to contain an iron‐binding site that can initiate magnetite crystal nucleation and control the size and shape of magnetite particles in vitro . Recently it was demonstrated that Mms6 binds to ferric and ferrous ions rather than to magnetite surfaces, and is a nucleating agent rather than a controller of magnetite crystal growth . Mms6 attached to surfaces has been used for the patterned formation of magnetite .…”

Section: Introductionmentioning

confidence: 99%

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Magnetite‐Binding Flagellar Filaments Displaying the MamI Loop Motif

et al. 2016

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This work aimed at developing a novel method for fabricating 1 D magnetite nanostructures with the help of mutated flagellar filaments. We constructed four different flagellin mutants displaying magnetite-binding motifs: two contained fragments of magnetosome-associated proteins from magnetotactic bacteria (MamI and Mms6), and synthetic sequences were used for the other two. A magnetic selection method identified the MamI mutant as having the highest binding affinity to magnetite. Filaments built from MamI loop-containing flagellin subunits were used as templates to form chains of magnetite nanoparticles along the filament by capturing them from suspension. Our study represents a proof-of-concept that flagellar filaments can be engineered to facilitate formation of 1 D magnetite nanostructures under ambient conditions. In addition, it proves the interaction between MamI and magnetite, with implications for the role of this protein in magnetotactic bacteria.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

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Magnetite‐Binding Flagellar Filaments Displaying the MamI Loop Motif

et al. 2016

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“…Due to its amphiphilic sequence, purified Mms6 self‐assembles into micellar structures . These large aggregates are able to both bind and accumulate ferric ions from solution, and purified assemblies of Mms6 on a biomimetic magnetosome interior surface demonstrate magnetite formation properties, indicating that Mms6 can act as a potential iron oxide nucleation site for subsequent crystal formation . In addition, the acid rich C‐terminal part of Mms6 has been studied and was found to exhibit some similar characteristics to the full‐length protein such as iron binding and a limited ability to affect magnetite crystal growth …”

Section: Introductionmentioning

confidence: 99%

Ferrous Iron Binding Key to Mms6 Magnetite Biomineralisation: A Mechanistic Study to Understand Magnetite Formation Using pH Titration and NMR Spectroscopy

Rawlings

Bramble

Hounslow

et al. 2016

Chemistry A European J

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Formation of magnetite nanocrystals by magnetotactic bacteria is controlled by specific proteins which regulate the particles’ nucleation and growth. One such protein is Mms6. This small, amphiphilic protein can self‐assemble and bind ferric ions to aid in magnetite formation. To understand the role of Mms6 during in vitro iron oxide precipitation we have performed in situ pH titrations. We find Mms6 has little effect during ferric salt precipitation, but exerts greatest influence during the incorporation of ferrous ions and conversion of this salt to mixed‐valence iron minerals, suggesting Mms6 has a hitherto unrecorded ferrous iron interacting property which promotes the formation of magnetite in ferrous‐rich solutions. We show ferrous binding to the DEEVE motif within the C‐terminal region of Mms6 by NMR spectroscopy, and model these binding events using molecular simulations. We conclude that Mms6 functions as a magnetite nucleating protein under conditions where ferrous ions predominate.

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“…In their very recent study, they have used a similar biomimetic system to investigate the differences between the Mms6 C-terminal peptide and the intact Mms6 protein in magnetite synthesis. [298] …”

Section: Protein Mediated Synthesis Of Magnetite Nanoparticlesmentioning

confidence: 99%

Synthesis, Functionalization, and Design of Magnetic Nanoparticles for Theranostic Applications

Mosayebi

Kiyasatfar

Laurent

2017

Adv Healthcare Materials

192

171

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In order to translate nanotechnology into medical practice, magnetic nanoparticles (MNPs) have been presented as a class of non‐invasive nanomaterials for numerous biomedical applications. In particular, MNPs have opened a door for simultaneous diagnosis and brisk treatment of diseases in the form of theranostic agents. This review highlights the recent advances in preparation and utilization of MNPs from the synthesis and functionalization steps to the final design consideration in evading the body immune system for therapeutic and diagnostic applications with addressing the most recent examples of the literature in each section. This study provides a conceptual framework of a wide range of synthetic routes classified mainly as wet chemistry, state‐of‐the‐art microfluidic reactors, and biogenic routes, along with the most popular coating materials to stabilize resultant MNPs. Additionally, key aspects of prolonging the half‐life of MNPs via overcoming the sequential biological barriers are covered through unraveling the biophysical interactions at the bio–nano interface and giving a set of criteria to efficiently modulate MNPs' physicochemical properties. Furthermore, concepts of passive and active targeting for successful cell internalization, by respectively exploiting the unique properties of cancers and novel targeting ligands are described in detail. Finally, this study extensively covers the recent developments in magnetic drug targeting and hyperthermia as therapeutic applications of MNPs. In addition, multi‐modal imaging via fusion of magnetic resonance imaging, and also innovative magnetic particle imaging with other imaging techniques for early diagnosis of diseases are extensively provided.

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Using a biomimetic membrane surface experiment to investigate the activity of the magnetite biomineralisation protein Mms6

Abstract: Using a surface-based mimic of a magnetosome interior, the biomineralisation protein Mms6 was found to be a more effective nucleator than binder of magnetite nanoparticles, and performs better than its C-terminal region alone.

Cited by 34 publications

References 35 publications

Magnetite‐Binding Flagellar Filaments Displaying the MamI Loop Motif

Magnetite‐Binding Flagellar Filaments Displaying the MamI Loop Motif

Ferrous Iron Binding Key to Mms6 Magnetite Biomineralisation: A Mechanistic Study to Understand Magnetite Formation Using pH Titration and NMR Spectroscopy

Synthesis, Functionalization, and Design of Magnetic Nanoparticles for Theranostic Applications

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