USF2 reduces BMP3 expression via transcriptional activation of miR-34a, thus promoting osteogenic differentiation of BMSCs

Zeng, Hanbing; Dong, Liqiang; Huang, Yanyun; Xu, Chao; Xiao-yan, Zhao; Wu, Lianguo

doi:10.1007/s00774-021-01254-x

Cited by 6 publications

(9 citation statements)

References 26 publications

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“…For example, USF2, as a transcriptional regulator of ATF4, was involved in the osteogenic differentiation of PDLCs 19 . Moreover, USF2 promoted osteogenic differentiation of BMSCs by reducing BMP3 expression 20 . Our study demonstrated that USF2 could target the promoter of WDR72 and induce its expression.…”

Section: Discussionmentioning

confidence: 60%

“…The JASPAR database further revealed that there were potential complementary binding sites between the transcription factor USF2 and the WDR72 promoter region (Figure 4A, right). Interestingly, studies have shown that USF2 promotes the osteogenic differentiation of PDLCs and BMSCs 19,20 . Therefore, USF2 was selected for further verification.…”

Section: Resultsmentioning

confidence: 99%

“…Human PDLSCs were obtained from healthy periodontal ligament tissues of premolars extracted for orthodontic treatment from 18 healthy individuals (9 males, 9 females, age [19][20][21][22][23][24][25][26][27] in Henan Provincial People's Hospital. All volunteers provided written, informed consent.…”

Section: Extraction and Identification Of Human Pdlscsmentioning

confidence: 99%

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Long non‐coding RNA X‐Inactive Specific Transcript (XIST) interacting with USF2 promotes osteogenic differentiation of periodontal ligament stem cells through regulation of WDR72 transcription

Xu,

Li,

Ren

et al. 2023

J of Periodontal Research

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BackgroundPeriodontal ligament stem cells (PDLSCs) are the most potential cells in periodontal tissue regeneration and bone tissue regeneration. Our prior work had revealed that WD repeat‐containing protein 72 (WDR72) was crucial for osteogenic differentiation of PDLSCs. Here, we further elucidated its underlying mechanism in PDLSC osteogenic differentiation.MethodsHuman PDLSCs, isolated and identified by flow cytometry, were prepared for osteogenic differentiation induction. Levels of WDR72, long non‐coding RNA X‐Inactive Specific Transcript (XIST), upstream stimulatory factor 2 (USF2), and osteogenic marker genes (Runx2, Osteocalcin, and Collagen I) in human PDLSCs and clinical specimens were detected by RT‐qPCR. Protein expressions of WDR72, Runx2, Osteocalcin, and Colla1 were tested by Western blot. The interactions among the molecules were verified by RIP, RNA pull‐down, ChIP, and luciferase reporter assays. Osteogenic differentiation was evaluated by alkaline phosphatase (ALP) and alizarin red staining (ARS).ResultsWDR72 was decreased in periodontal tissues of periodontitis patients, and overexpression reversed TNF‐α‐mediated suppressive effects on PDLSC osteogenic differentiation. Mechanically, XIST recruited the enrichment of USF2 to the WDR72 promoter region, thereby positively regulating WDR72. WDR72 silencing overturned XIST‐mediated biological effects in PDLSCs.ConclusionWDR72, regulated by the XIST/USF2 axis, enhances osteogenic differentiation of PDLSCs, implying a novel strategy for alleviating periodontitis.

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Section: Discussionmentioning

confidence: 60%

Section: Resultsmentioning

confidence: 99%

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Long non‐coding RNA X‐Inactive Specific Transcript (XIST) interacting with USF2 promotes osteogenic differentiation of periodontal ligament stem cells through regulation of WDR72 transcription

Xu,

Li,

Ren

et al. 2023

J of Periodontal Research

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“…BMP3 is the most abundant member of BMP family, accounting for about 65% of the total content ( Bahamonde and Lyons, 2001 ). MiR-34a promotes the osteogenic differentiation of BMSCs by directly targeting BMP3 ( Zeng et al, 2021 ). MiR-19b significantly promotes the osteogenic differentiation of BMSCs by targeting WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) and Samd ubiquitin regulatory factor 2 (SMURF2) through the kruppel like factor (KLF) 5/β-catenin signaling pathway ( Huang et al, 2021 ) ( Figure 2 and Table 1 ).…”

Section: Mirnas Involved In the Osteogenic Differentiation Of Bmscsmentioning

confidence: 99%

“…Postmenopausal osteoporosis (PMOP) is a common type of OP caused by estrogen deficiency. The osteogenic differentiation potential of BMSCs is compromised in OP patients ( Zeng et al, 2021 ). The expression of miR-486-3p is significantly downregulated in the bone marrow of OP patients.…”

Section: Mirnas Involved In the Osteogenic Differentiation Of Bmscsmentioning

confidence: 99%

The Emerging Role of Non-Coding RNAs in Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells

Chen

Xie

Zhang

et al. 2022

Front. Cell Dev. Biol.

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Autologous bone marrow-derived mesenchymal stem cells (BMSCs) are more easily available and frequently used for bone regeneration in clinics. Osteogenic differentiation of BMSCs involves complex regulatory networks affecting bone formation phenomena. Non-coding RNAs (ncRNAs) refer to RNAs that do not encode proteins, mainly including microRNAs, long non-coding RNAs, circular RNAs, piwi-interacting RNAs, transfer RNA-derived small RNAs, etc. Recent in vitro and in vivo studies had revealed the regulatory role of ncRNAs in osteogenic differentiation of BMSCs. NcRNAs had both stimulatory and inhibitory effects on osteogenic differentiation of BMSCs. During the physiological condition, osteo-stimulatory ncRNAs are upregulated and osteo-inhibitory ncRNAs are downregulated. The opposite effects might occur during bone degenerative disease conditions. Intracellular ncRNAs and ncRNAs from neighboring cells delivered via exosomes participate in the regulatory process of osteogenic differentiation of BMSCs. In this review, we summarize the recent advances in the regulatory role of ncRNAs on osteogenic differentiation of BMSCs during physiological and pathological conditions. We also discuss the prospects of the application of modulation of ncRNAs function in BMSCs to promote bone tissue regeneration in clinics.

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USF2 activates RhoB/ROCK pathway by transcriptional inhibition of miR-206 to promote pyroptosis in septic cardiomyocytes

et al. 2023

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USF2 reduces BMP3 expression via transcriptional activation of miR-34a, thus promoting osteogenic differentiation of BMSCs

Cited by 6 publications

References 26 publications

Long non‐coding RNA X‐Inactive Specific Transcript (XIST) interacting with USF2 promotes osteogenic differentiation of periodontal ligament stem cells through regulation of WDR72 transcription

Long non‐coding RNA X‐Inactive Specific Transcript (XIST) interacting with USF2 promotes osteogenic differentiation of periodontal ligament stem cells through regulation of WDR72 transcription

The Emerging Role of Non-Coding RNAs in Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells

USF2 activates RhoB/ROCK pathway by transcriptional inhibition of miR-206 to promote pyroptosis in septic cardiomyocytes

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