Usefulness of transabdominal real-time sonoelastography in the evaluation of ovarian lesions: preliminary results

Herek, Duygu; Karabulut, Aysun; Ağladıoğlu, Kadir

doi:10.1259/bjr.20160173

Cited by 9 publications

(8 citation statements)

References 22 publications

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“…Several studies have indicated that ovarian cancer development involves mechanical stimuli, although the molecular mechanisms responsible for the translation of mechanical forces into biochemical signals remain largely unexplored to date. Some clinical results have confirmed that most malignant lesions of the ovary are soft and have a very small strain ratio compared with normal lesions [ 19 , 20 ], and low-grade serous ovarian carcinomas are stiffer than high-grade lesions in vivo [ 21 ]. However, in cells, there are many similarities in the progression between breast and ovarian cancer, e.g.…”

Section: Introductionmentioning

confidence: 99%

RNA-seq reveals the diverse effects of substrate stiffness on epidermal ovarian cancer cells

Yang

Wang

Huang

et al. 2020

aging

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Background: Increasing evidence has confirmed that ovarian cancer is a mechanically responsive tumor both in vivo and in vitro . However, an understanding of the complete molecular mechanism involved in the response to substrate stiffness is lacking, as the associated transcriptome-wide effects have not been mapped. This limited understanding has restricted the identification of potential mechanically responsive targets in ovarian cancer. Results: To address these limitations, we used a polyacrylamide hydrogel system with a tunable Young’s modulus that broadly ranged from soft (1 kPa) to normal (6 kPa) and stiff (60 kPa) and investigated the effect of substrate rigidity on the morphology, spreading area, and cytoskeleton of SKOV-3 epidermal ovarian cancer (EOC) cells. RNA-seq analysis of these cells was then performed at appropriate timepoints to map the transcriptome-wide changes associated with stiffness sensing. We identified a large number of stiffness-sensing genes as well as many genes that were enriched in cancer-related pathways. Informed by these diverse expression results and based on bioinformatics analysis, we evaluated the hypothesis that PLEC and TNS2, which are located in focal adhesions and regulated by lnc-ZNF136, may play key roles in the EOC response to substrate stiffness. Conclusion: Overall, the results of the present study reveal previously unknown features of the EOC stiffness response and provide new insights into EOC metastasis in the clinic.

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Section: Introductionmentioning

confidence: 99%

RNA-seq reveals the diverse effects of substrate stiffness on epidermal ovarian cancer cells

Yang

Wang

Huang

et al. 2020

aging

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“…This work contributes to a larger body of work on measuring the mechanical properties of the ovary 31,39,40,46,[54][55][56][57][58][59][60] and related specialized ECMs, the cumulus oophorus 61,62 and zona pellucida. 63,64 The mechanical properties of mammalian ovary and related cells and ECMs have been investigated using numerous techniques, including nanoindentation, SW elastography (ultrasound and MR), tensile testing, and rheology.…”

Section: Limitationsmentioning

confidence: 99%

“…MR elastography is still the preferred method for evaluation of ovarian tissue in pediatric patients, 31 where the transvaginal approach is inappropriate, although some groups have had success with transabdominal SW ultrasound elastography of the ovary. 59 We expect to see SW ultrasound elastography continue its role in the diagnosis of adnexal masses, 39,56,59 thereby reducing the need for unnecessary surgical procedures and in the prognosis of ovarian cancer. 56,57 It remains inconclusive if a difference in SW propagation exists between ovaries from women with PCOS and normally ovulating women.…”

Section: Clinical Applicationsmentioning

confidence: 99%

Ultrasound Shear Wave Velocity Varies Across Anatomical Region in Ex Vivo Bovine Ovaries

Gargus

Jakubowski

Arenas

et al. 2020

Tissue Engineering Part A

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The physical properties of the ovarian extracellular matrix (ECM) regulate the function of ovarian cells, specifically the ability of the ovary to maintain a quiescent primordial follicle pool while allowing a subset of follicles to grow and mature in the estrous cycle. Design of a long-term, cycling artificial ovary has been hindered by the limited information regarding the mechanical properties of the ovary. In particular, differences in the mechanical properties of the two ovarian compartments, the cortex and medulla, have never been quantified. Shear wave (SW) ultrasound elastography is an imaging modality that enables assessment of material properties, such as the mechanical properties, based on the velocity of SWs, and visualization of internal anatomy, when coupled with B-mode ultrasound. We used SW ultrasound elastography to assess whole, ex vivo bovine ovaries. We demonstrated, for the first time, a difference in mechanical properties, as inferred from SW velocity, between the cortex and medulla, as measured along the length (cortex: 2.57-0.53 m/s, medulla: 2.87-0.77 m/s, p < 0.0001) and width (cortex: 2.99-0.81 m/s, medulla: 3.24-0.97 m/s, p < 0.05) and that the spatial distribution and magnitude of SW velocity vary between these two anatomical planes. This work contributes to a larger body of literature assessing the mechanical properties of the ovary and related cells and specialized ECMs and will enable the rational design of biomimetic tissue engineered models and durable bioprostheses. Impact Statement Shear wave (SW) ultrasound elastography can be used to simultaneously assess the material properties and tissue structures when accompanied with B-mode ultrasound. We report a quantitative difference in mechanical properties, as inferred from SW velocity, between the cortex and medulla, with SW velocity being 11.4% and 8.4% higher in the medulla than the cortex when measured along the length and width, respectively. This investigation into the spatial and temporal variation in SW velocity in bovine ovaries will encourage and improve design of more biomimetic scaffolds for ovarian tissue engineering.

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“…30,31 Benign tumors are reported to have an average stiffness between malignant and healthy lesions. 32 Ovarian tissue is a proper candidate for the study of changes in tissue and cell elasticity values related to the disease development since there are diverse types of ovarian tumors with possibly different mechanical properties depending on the disease character and progress. Hence, in this study, we examined the extent of changes on mechanical properties of cellular and non-cellular parts of diseased ovarian tissues with different types of tumors and cysts.…”

Section: Introductionmentioning

confidence: 99%

<p>An AFM-Based Nanomechanical Study of Ovarian Tissues with Pathological Conditions</p>

Ansardamavandi¹,

Tafazzoli-Shadpour²,

Omidvar³

et al. 2020

IJN

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Background: Different diseases affect both mechanical and chemical features of the involved tissue, enhancing the symptoms. Methods: In this study, using atomic force microscopy, we mechanically characterized human ovarian tissues with four distinct pathological conditions: mucinous, serous, and mature teratoma tumors, and non-tumorous endometriosis. Mechanical elasticity profiles were quantified and the resultant data were categorized using K-means clustering method, as well as fuzzy C-means, to evaluate elastic moduli of cellular and non-cellular parts of diseased tissues and compare them among four disease conditions. Samples were stained by hematoxylin-eosin staining to further study the content of different locations of tissues. Results: Pathological state vastly influenced the mechanical properties of the ovarian tissues. Significant alterations among elastic moduli of both cellular and non-cellular parts were observed. Mature teratoma tumors commonly composed of multiple cell types and heterogeneous ECM structure showed the widest range of elasticity profile and the stiffest average elastic modulus of 14 kPa. Samples of serous tumors were the softest tissues with elastic modulus of only 400 Pa for the cellular part and 5 kPa for the ECM. Tissues of other two diseases were closer in mechanical properties as mucinous tumors were insignificantly stiffer than endometriosis in cellular part, 1300 Pa compared to 1000 Pa, with the ECM average elastic modulus of 8 kPa for both. Conclusion: The higher incidence of carcinoma out of teratoma and serous tumors may be related to the intense alteration of mechanical features of the cellular and the ECM, serving as a potential risk factor which necessitates further investigation.

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Usefulness of transabdominal real-time sonoelastography in the evaluation of ovarian lesions: preliminary results

Cited by 9 publications

References 22 publications

RNA-seq reveals the diverse effects of substrate stiffness on epidermal ovarian cancer cells

RNA-seq reveals the diverse effects of substrate stiffness on epidermal ovarian cancer cells

Ultrasound Shear Wave Velocity Varies Across Anatomical Region in Ex Vivo Bovine Ovaries

<p>An AFM-Based Nanomechanical Study of Ovarian Tissues with Pathological Conditions</p>

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