Usefulness of temperate-adapted maize lines developed by doubled haploid and single-seed descent methods

Santos, Iara Gonçalves dos; Verzegnazzi, Anderson Luiz; Edwards, Jode W.; Frei, Ursula; Boerman, Nicholas A.; Zuffo, Leandro Tonello; Pires, Luíz Paulo Miranda; Fuente, Gerald de La; Lübberstedt, Thomas

doi:10.1007/s00122-022-04075-2

Cited by 3 publications

(3 citation statements)

References 42 publications

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Section: Methodsmentioning

confidence: 99%

“…A panel of 793 non-inducer genotypes was considered for a case-control GWAS (cc-GWAS) (Table S1). This panel was composed mainly of DH or highly homozygous lines derived from various genetic backgrounds, such as B73, BS39 (Verzegnazzi et al, 2021;Santos et al, 2022), several cycles of the Iowa Stiff Stalk Synthetic Population (BSSS) (Ledesma et al, in preparation), BGEM (Iowa State University/USDA-ARS Germplasm Enhancement Maize project) and Ex-PVP lines. No haploid plants were observed when these genotypes were grown in the field, which is in accordance with Chase (1947) observation that the occurrence of haploid plants in elite inbred lines happens at very low frequencies (<0.1%).…”

Section: Germplasmmentioning

confidence: 99%

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Genetic basis of maize maternal haploid induction beyond MATRILINEAL and ZmDMP

Trentin,

Krause,

Zunjare

et al. 2023

Front. Plant Sci.

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In maize, doubled haploid (DH) lines are created in vivo through crosses with maternal haploid inducers. Their induction ability, usually expressed as haploid induction rate (HIR), is known to be under polygenic control. Although two major genes (MTL and ZmDMP) affecting this trait were recently described, many others remain unknown. To identify them, we designed and performed a SNP based (~9007) genome-wide association study using a large and diverse panel of 159 maternal haploid inducers. Our analyses identified a major gene near MTL, which is present in all inducers and necessary to disrupt haploid induction. We also found a significant quantitative trait loci (QTL) on chromosome 10 using a case-control mapping approach, in which 793 noninducers were used as controls. This QTL harbors a kokopelli ortholog, whose role in maternal haploid induction was recently described in Arabidopsis. QTL with smaller effects were identified on six of the ten maize chromosomes, confirming the polygenic nature of this trait. These QTL could be incorporated into inducer breeding programs through marker-assisted selection approaches. Further improving HIR is important to reduce the cost of DH line production.

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Section: Methodsmentioning

confidence: 99%

Section: Germplasmmentioning

confidence: 99%

Genetic basis of maize maternal haploid induction beyond MATRILINEAL and ZmDMP

Trentin,

Krause,

Zunjare

et al. 2023

Front. Plant Sci.

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“…Stadler and Randolph (1929) reported the rst spontaneous haploid in maize. Doubled haploid technology has facilitated studies at the molecular (Trindade et al, 2022) and genomic level and simpli ed logistics to make the maize breeding process more e cient and intuitive, increased genetic gains in different breeding programs (Santos et al, 2022). DH technology has emerged as the potential technology to enhance hybrid seed production through rapid generation of elite inbreds (Eliby et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

In vitro Production of Double Haploid in Maize (Zea mays L.) Through Pollen Culture

Muneer

Khan

Naz

et al. 2023

Preprint

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Maize (Zea mays L.) has a distinct genetic system and is recalcitrant to generate haploid and subsequently double haploid plants from pollen grains in aseptic conditions. It is a technique with lot of potential for producing pure lines for crop improvement and introducing the desired variability through modification in biochemical and applied genetics. The experiment was conducted using different doses of 2,4-Dichlorophenoxyacetic acid (2, 4-D), Indole-3-acetic acid (IAA), 6-Benzylaminopurine(BAP), Zeatin(ZEA), Kinetin and Naphthalene Acetic Acid(NAA) ranging from 0.5 mg/L to 5 mg/L for the callus induction and plantlet regeneration. The callus was sub-cultured for double haploid induction with doses of 100–500 mg/l colchicine for 7h, 12h and 7 days. Microscopic examination and morphological identification were proposed to check ploidy level. The results exhibited the callus formation at 1, 2 and 3 mg/l concentrations of 2, 4-D and IAA respectively. Based on the time required for the 2, 4-D, callus induction at the concentration of 2mg/l exhibited (47 days) and at the same concentration of IAA exhibited the minimum days of callus induction (34 days). The colchicine treated pollen derived callus regenerated into roots and shoots on IAA and BAP with 3mg/l and 2mg/l respectively. In contrast to pollen derived callus, colchicine treated seed callus gave better results. Double haploid plant produced successfully through in vitro culture of pollen. Current study will be helpful for the achievement of homozygosity in relatively less time and varietal improvement for desired traits.

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