“…An additional advantage of the molecular techniques is their rapidity in that culture of isolates is no more needed. On the other hand, they are much adapted for both diagnostic (PCR, Multiplex PCR, Real time-PCR) and epidemiological (PCR-RFLP, Multiplex PCR, RAPD) purposes (Aoun et al, 2008;Bel Hadj et al, 2002;Ben Said et al, 2006;Chargui et al, 2005;Guerbouj et al, 2007;Guizani et al, 1994aGuizani et al, , 1994bGuizani et al, 2002;Hanafi et al, 2001;Saadi et al, in preparation). In addition, molecular techniques are specific and highly sensitive, allowing identification of parasite species hard to isolate or to maintain in vitro in culture or too scarce to be detected or cultured.…”