Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates

Baldwin, Carson; Howe, Gerald B.; Sampath, Ranga; Blyn, Larry B.; Matthews, Heather; Harpin, Vanessa; Hall, Thomas A.; Drader, Jared J.; Hofstadler, Steve; Eshoo, Mark W.; Rudnick, Karl; Studarus, Karen; Moore, D. J.; Abbott, Sharon L.; Janda, J. Michael; Whitehouse, Chris A.

doi:10.1016/j.diagmicrobio.2008.12.012

Cited by 55 publications

(34 citation statements)

References 20 publications

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“…This method enables detection and identification of causative pathogens directly from clinical specimens or mixtures of organisms with rapid turnaround and high throughput (9,10). This technique has been used to detect bacteria, viruses, yeasts, and other infectious agents (10)(11)(12)(13), but there are no data on its value for the detection of fungal organisms, including yeasts and molds.…”

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confidence: 99%

Detection, Identification, and Distribution of Fungi in Bronchoalveolar Lavage Specimens by Use of Multilocus PCR Coupled with Electrospray Ionization/Mass Spectrometry

et al. 2013

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dAs pulmonary fungal infections continue to increase due to an increasing number of immunocompromised patients, rapid detection and accurate identification of these fungal pathogens are critical. A broad fungal assay was developed by incorporating broad-range multilocus PCR amplification and electrospray ionization/mass spectrometry (PCR/ESI-MS) to detect and identify fungal organisms directly from clinical specimens. The aims of this study were to evaluate the performance of PCR/ESI-MS for detection, identification, and determination of the distribution of fungal organisms in bronchoalveolar lavage (

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confidence: 99%

Detection, Identification, and Distribution of Fungi in Bronchoalveolar Lavage Specimens by Use of Multilocus PCR Coupled with Electrospray Ionization/Mass Spectrometry

et al. 2013

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“…for the presence or absence of the genetic targets (carbapenemase genes) that are known to be associated with CR, as previously described (13,14). In brief, PCR/ESI-MS is a nucleic acid amplification technology that targets select genes using "smart primers," determines their exact mass, and then uses algorithms to define the target gene identified (1).…”

Section: Methodsmentioning

confidence: 99%

Informing Antibiotic Treatment Decisions: Evaluating Rapid Molecular Diagnostics To Identify Susceptibility and Resistance to Carbapenems against Acinetobacter spp. in PRIMERS III

et al. 2017

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The widespread dissemination of carbapenem-resistant Acinetobacter spp. has created significant therapeutic challenges. At present, rapid molecular diagnostics (RMDs) that can identify this phenotype are not commercially available. Two RMD platforms, PCR combined with electrospray ionization mass spectrometry (PCR/ ESI-MS) and molecular beacons (MB), for detecting genes conferring resistance/susceptibility to carbapenems in Acinetobacter spp. were evaluated. An archived collection of 200 clinical Acinetobacter sp. isolates was tested. Predictive values for susceptibility and resistance were estimated as a function of susceptibility prevalence and were based on the absence or presence of beta-lactamase (bla) NDM, VIM, IMP, KPC, and OXA carbapenemase genes (e.g., bla , bla , and bla OXA-58 found in this study) against the reference standard of MIC determinations. According to the interpretation of MICs, 49% (n ϭ 98) of the isolates were carbapenem resistant (as defined by either resistance or intermediate resistance to imipenem). The susceptibility sensitivities (95% confidence interval [CI]) for imipenem were 82% (74%, 89%) and 92% (85%, 97%) for PCR/ESI-MS and MB, respectively. Resistance sensitivities (95% CI) for imipenem were 95% (88%, 98%) and 88% (80%, 94%) for PCR/ESI-MS and MB, respectively. PRIMERS III establishes that RMDs can discriminate between carbapenem resistance and susceptibility in Acinetobacter spp. In the context of a known prevalence of resistance, SPVs and RPVs can inform clinicians regarding the best choice for empiric antimicrobial therapy against this multidrug-resistant pathogen.

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“…All bacterial culture attempts from patients' blood and camel tissue were negative or only yielded contaminants, and few samples were available for testing ; the key to identifying the causative agent in this outbreak was the use of the Ibis T5000 PCR/ESI-MS assay. This diagnostic tool has previously been shown to detect a broad range of bacterial pathogens [16,17] and several viruses [18][19][20]. The inclusion of certain key gene targets in the Ibis T5000 biodefence assay allows it to detect and identify a wide range of pathogens in potentially complex clinical or environmental samples with little or no background effect.…”

Section: Discussionmentioning

confidence: 99%

Outbreak of gastroenteritis caused byYersinia pestisin Afghanistan

et al. 2010

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SUMMARYPlague, which is most often caused by the bite of Yersinia pestis-infected fleas, is a rapidly progressing, serious disease that can be fatal without prompt antibiotic treatment. In late December 2007, an outbreak of acute gastroenteritis occurred in Nimroz Province of southern Afghanistan. Of the 83 probable cases of illness, 17 died (case fatality 20 . 5 %). Being a case was associated with consumption or handling of camel meat (adjusted odds ratio 4 . 4, 95% confidence interval 2 . 2-8 . 8, P<0 . 001). Molecular testing of patient clinical samples and of tissue from the camel using PCR/electrospray ionization-mass spectrometry revealed DNA signatures consistent with Yersinia pestis. Confirmatory testing using real-time PCR and immunological seroconversion of one of the patients confirmed that the outbreak was caused by plague, with a rare gastrointestinal presentation. The study highlights the challenges of identifying infectious agents in low-resource settings ; it is the first reported occurrence of plague in Afghanistan.

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Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates

Cited by 55 publications

References 20 publications

Detection, Identification, and Distribution of Fungi in Bronchoalveolar Lavage Specimens by Use of Multilocus PCR Coupled with Electrospray Ionization/Mass Spectrometry

Detection, Identification, and Distribution of Fungi in Bronchoalveolar Lavage Specimens by Use of Multilocus PCR Coupled with Electrospray Ionization/Mass Spectrometry

Informing Antibiotic Treatment Decisions: Evaluating Rapid Molecular Diagnostics To Identify Susceptibility and Resistance to Carbapenems against Acinetobacter spp. in PRIMERS III

Outbreak of gastroenteritis caused byYersinia pestisin Afghanistan

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