Usefulness of molecular genetic markers in the typing of Salmonella enterica serovar Enteritidis causing a food-borne outbreak

Ma, Gonzalez-Hevia; Jj, Llaneza; Mc, Mendoza

doi:10.1016/0168-1605(94)90134-1

Cited by 8 publications

(6 citation statements)

References 13 publications

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“…Molecular approaches which 'interrogate' the whole chromosome appear to be the way forward in order to highlight what may be only minimal differences between strains. Such methods include pulsed-field gel electrophoresis (PFGE) (Powell et al 1994;Threlfall et al 1996), restriction enzyme analysis (Gonzalez-Hevia et al 1994) and RAPD (Welsh and McClelland 1990;Williams et al 1990). Targeting the limited amount of polymorphism within highly clonal strains can be extremely difficult and so even these methods have met with limited success.…”

Section: Discussionmentioning

confidence: 99%

Restriction enzyme analsys of randomly ampliefied polymorphic DNA amplicons of Salmonella enterica ser. Enteritidis PT4 and Typhimurium DT104

Hilton

Penn

1998

Letters in Applied Microbiology

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Salmonella enterica serotype Enteritidis PT4 and Typhimurium DT104 isolates were characterized using a random amplification of polymorphic DNA (RAPD) protocol found previously to be highly discriminatory for isolates of Salmonella. Profiles generated with a single primer 1254, and independently 1283, successfully characterized an outbreak strain of Enteritidis PT4 but could not differentiate epidemiologically unrelated strains of Enteritidis PT4 from the outbreak strains. Primer 1254 differentiated one strain, and 1283 two strains of Typhimurium DT104 previously undifferentiated on the basis of biochemical and physical properties. Subsequent analysis using a combination of RAPD and restriction enzyme analysis could not provide additional differentiation of Enteritidis PT4 and Typhimurium DT104 isolates but did, however, exhibit the potential to be a useful combination of molecular techniques.

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Section: Discussionmentioning

confidence: 99%

Restriction enzyme analsys of randomly ampliefied polymorphic DNA amplicons of Salmonella enterica ser. Enteritidis PT4 and Typhimurium DT104

Hilton

Penn

1998

Letters in Applied Microbiology

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“…Frost et al (5) demonstrated conversion of S. enterica serovar Enteritidis PT 4 to PT 24 based on the acquisition of a plasmid. Subsequently, Threlfall et al (25) have shown interrelationships between PT 4,7,7a,8,13,13a,23,24, and 30 caused by the loss or acquisition of an incN plasmid. Recently, Rankin and Platt (18) demonstrated that S. enterica serovar Enteritidis PT may be changed as a result of phage conversion by the temperate phages 1, 2, 3, and 6 from the phage typing scheme of Ward et al (27).…”

Section: Studies Of Pairs Of Salmonella Enterica Serovar Enteritidis mentioning

confidence: 99%

Correlation of conversion of Salmonella enterica serovar enteritidis phage type 1, 4, or 6 to phage type 7 with loss of lipopolysaccharide

Baggesen¹,

Wegener²,

Madsen³

1997

J Clin Microbiol

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“…In addition, there were three isolates from pork meat implicated in one of the outbreaks, three from drinking water and six from treated sewage water. Four Typhimurium strains from other collections were used as reference strains (Table 1); one strain (93/92) of Enteritidis belonging to the prevalent clonal line in the Principality of Asturias [13] was used as an outgroup strain.…”

Section: P3mentioning

confidence: 99%

A three-way ribotyping scheme for Salmonella serotype Typhimurium and its usefulness for phylogenetic and epidemiological purposes

Guerra

Landeras

González-Hevia

et al. 1997

Journal of Medical Microbiology

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Ribotyping of Salmonella serotype Typhimurium strains was optimised as a tool for epidemiological and phylogenetic purposes. Of five restriction endonucleases evaluated on a series of 84 isolates, HincII, San and PvuII were the most useful, generating 13, 9 and 9 ribotypes with 17, 11 and 18 polymorphic restriction sites, and attaining a discrimination index (DI) of 0.81, 0.53 and 0.59, respectively. The combination of results from tests with the three enzymes provided further discrimination (19 ribotypes, DI = 0.84). It proved useful for clonal analysis, defining 19 clonal lines with a remarkable degree of genetic heterogeneity, that were grouped into two major clusters (including 12 and 7 lines, respectively) at a significance level of 0.65. When the attributes of this system were compared with those of phage typing, it was found that ribotyping showed higher typability and sensitivity, supporting its use as an appropriate molecular method. In tracing the molecular epidemiology of Typhimurium strains in Asturias, six lines were found that could be considered endemic and were represented by organisms implicated in salmonellosis throughout the period of study; another four lines included organisms isolated from meat, water or both.

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Usefulness of molecular genetic markers in the typing of Salmonella enterica serovar Enteritidis causing a food-borne outbreak

Cited by 8 publications

References 13 publications

Restriction enzyme analsys of randomly ampliefied polymorphic DNA amplicons of Salmonella enterica ser. Enteritidis PT4 and Typhimurium DT104

Restriction enzyme analsys of randomly ampliefied polymorphic DNA amplicons of Salmonella enterica ser. Enteritidis PT4 and Typhimurium DT104

Correlation of conversion of Salmonella enterica serovar enteritidis phage type 1, 4, or 6 to phage type 7 with loss of lipopolysaccharide

A three-way ribotyping scheme for Salmonella serotype Typhimurium and its usefulness for phylogenetic and epidemiological purposes

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