Usefulness of Modified Dixon's Medium for Quantitative Culture of<i>Malassezia</i>Species from Canine Skin

Guillot, Jacques; Breugnot, Caroline; Barros, Manjula de; Chermette, René

doi:10.1177/104063879801000418

Cited by 31 publications

(35 citation statements)

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“…The remaining 56.19% (n=59) isolates could not be identified at the species level, given the profiles did not coincide with those reported in the literature (11,14,19), nor with reactions presented for CBS reference strains. h o l o g i c a l , m a c r o s c o p i c , a n (4), 78.3% (n=29) ( Table 1) of isolates obtained from dogs in the clinic were identified as M. pachydermatis, they described opaque witish colonies, sometimes umbilicated with an average size of 4 -5 mm (4).…”

Section: Discussionmentioning

confidence: 77%

“…El 56.19% (n=59) de los aislamientos no pudieron ser clasificados a nivel de especie, dado que su perfil no coincidió con lo reportado en la literatura (11,14,19), ni con las reacciones que presentaron las cepas de referencia CBS.…”

Section: Discussionunclassified

“…Por la condición no lípido-dependiente de M. pachydermatis, a diferencia de las demás especies, ésta tiene la capacidad de crecer en medios de cultivo sin suplementos lipídicos como agar Sabouraud (1,(11)(12)(13). Los medios utilizados para el aislamiento de las especies lipidodependientes son el agar Dixon modificado y el Leeming & Notman que contienen tween, glicerol y ácido oleico como fuentes lipídicas para el desarrollo de estas levaduras (11,12).…”

Section: Introductionunclassified

“…Los medios utilizados para el aislamiento de las especies lipidodependientes son el agar Dixon modificado y el Leeming & Notman que contienen tween, glicerol y ácido oleico como fuentes lipídicas para el desarrollo de estas levaduras (11,12).…”

Section: Introductionunclassified

“…This yeast can grow in culture media devoid of lipids, such as Sabouraud's agar (1,(11)(12)(13). Media utilized for obligatory lipophylic species isolation are modified Dixon agar and Leeming & Notman, containing Tween, glycerol, and oleic acid as lipid sources for yeast proliferation (11)(12).…”

Section: Introductionmentioning

confidence: 99%

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Caracterización fenotípica de aislamientos de Malassezia spp., de origen canino

et al. 2016

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Objective. To characterize and identify yeasts of the genus Malassezia by phenotypic features. Materials and methods. First, the macroscopic and microscopic morphological characteristics were described. In addition we performed biochemical and physiological assays as Tweens and Cremophor, including more. Results. Our results evidenced of 105 isolates obtained from dogs diagnosed with external otitis, it was possible to identify two distinct species from 46 isolates within the Malassezia genus: 36.19% (n=38) were identified as M. pachydermatis and 7.62% (n=8) as M. furfur. According to phenotypic patterns the remaining 56.19% (n=59) were reported as Malassezia spp., possibly corresponding to M. furfur and/or M. pachydermatis. Conclusions. Results emphasize the necessity to characterize according to species. It is not feasible to define Malassezia by species based on morphological, biochemical, and physiological findings. Therefore, molecular genotyping should be performed to identify markers allowing a more precise isolate identification. This would broaden our epidemiological knowledge regarding different species involved in canine otitis pathologies. Keywords: Biochemical techniques, external otitis, Malassezia, morphology. (Source: CAB) RESUMENObjetivo. Caracterizar e identificar levaduras del género Malassezia, mediante características fenotípicas. Materiales y métodos. Inicialmente se describieron las características morfológicas macroscópicas y microscópicas, adicionalmente se realizaron pruebas bioquímicas y fisiológicas como Tween y Cremophor, entre otras. Resultados. De 105 aislamientos de caninos diagnosticados previamente con otitis, 46 fueron caracterizados hasta especie, así: El 36.19% (n=38) correspondió a M. pachydermatis, el 7.2% (n=8) a M. furfur; y 56.19% (n=59) restante fueron reportados como Malassezia spp., debido a los patrones fenotípicos atípicos que presentaron, y que podrían corresponder a variantes de M. furfur y/o M. pachydermatis. Conclusión. Estos resultados enfatizan la necesidad de hacer una caracterización a nivel de especie y/o genotipos mediante marcadores moleculares que permitan una identificación más precisa de los aislamientos. Con el presente estudio, se contribuye al conocimiento de las diferentes especies involucradas en patologías óticas en caninos.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionunclassified

Section: Introductionunclassified

Section: Introductionmentioning

confidence: 99%

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Caracterización fenotípica de aislamientos de Malassezia spp., de origen canino

et al. 2016

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Dermatomycoses

2011

Fundamental Medical Mycology

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Ketoconazole inhibits Malassezia furfur morphogenesis in vitro under filamentation optimized conditions

et al. 2016

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Malassezia furfur, a constituent of the normal human skin flora, is an etiological agent of pityriasis versicolor, which represents one of the most common human skin diseases. Under certain conditions, both exogenous and endogenous, the fungus can transition from a yeast form to a pathogenic mycelial form. To develop a standardized medium for reproducible production of the mycelial form of M. furfur to develop and optimize susceptibility testing for this pathogen, we examined and characterized variables, including kojic acid and glycine concentration, agar percentage, and pH, to generate a chemically defined minimal medium on which specific inoculums of M. furfur generated the most robust filamentation. Next, we examined the capacity of ketoconazole to inhibit the formation of M. furfur mycelial form. Both low and high, 0.01, 0.05 and 0.1 µg/ml concentrations of ketoconazole significantly inhibited filamentation at 11.9, 54.5 and 86.7%, respectively. Although ketoconazole can have a direct antifungal effect on both M. furfur yeast and mycelial cells, ketoconazole also has a dramatic impact on suppressing morphogenesis. Since mycelia typified the pathogenic form of Malassezia infection, the capacity of ketoconazole to block morphogenesis may represent an additional important effect of the antifungal.

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Usefulness of Modified Dixon's Medium for Quantitative Culture ofMalasseziaSpecies from Canine Skin

Cited by 31 publications

References 15 publications

Caracterización fenotípica de aislamientos de Malassezia spp., de origen canino

Caracterización fenotípica de aislamientos de Malassezia spp., de origen canino

Dermatomycoses

Ketoconazole inhibits Malassezia furfur morphogenesis in vitro under filamentation optimized conditions

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