Usefulness of microsatellite typing in population genetic studies of Trypanosoma cruzi

Macedo, Andréa Mara; Pimenta, Juliana R.; Aguiar, Renato Santana; Melo, Anna Izabel R.; Chiari, Égler; Zingales, Bianca; Pena, Sérgio D.J.; Oliveira, Riva P.

doi:10.1590/s0074-02762001000300023

Cited by 59 publications

(33 citation statements)

References 13 publications

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“…Several strains of TcI from Latin American countries have been analyzed with microsatellite analysis, and at least 2 subgroups have been established (Macedo et al 2001, Llewellyn et al 2009). In the present work, the analyzed TcI Mexican strains were grouped into 2 microsatellite genotypes.…”

Section: Discussionmentioning

confidence: 99%

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Microsatellite and Mini-Exon Analysis of Mexican Human DTU ITrypanosoma cruziStrains and Their Susceptibility to Nifurtimox and Benznidazole

Martı́nez

Nogueda

Martínez‐Hernández

et al. 2013

Vector-Borne and Zoonotic Diseases

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Chagas disease is caused by the protozoan parasite Trypanosoma cruzi, and it affects as many as 10 million people in North and South America, where it represents a major public health problem. T. cruzi is a parasite with high genetic diversity, and it has been grouped into 6 discrete typing units (DTUs), designated as T. cruzi I (TcI) to T. cruzi VI (TcVI). Mexican isolates from humans and from vector insects have been primarily found to be TcI, and these isolates are likely to be the strains that cause the clinical manifestations observed in Mexico. However, genetic characterization and drug susceptibility assays are limited in Mexican TcI strains. In this work, 24 Mexican T. cruzi strains, obtained primarily from humans, were studied with 7 locus microsatellites and mini-exon gene by PCR. Also, drug susceptibility was evaluated by growth and mobility assays. All of the human strains belonged to TcI, and they could be further grouped through microsatellite analysis into 2 subgroups (microsatellite genotypes 1 and 2), which were not related to the host clinical status or biological origin of the strain. Two strains, both from wild mammals, belonged to the TcII-TcVI groups; these strains and the CL Brener strain constituted microsatellite genotype 3. The number of alleles in each locus was lower than reported for South American strains, and a departure from the Hardy-Weinberg equilibrium was observed. The susceptibility of these strains to nifurtimox and benznidazole was heterogeneous. T. cruzi strains characterized as microsatellite genotypes 2 and 3 were significantly more susceptible to benznidazole than strains of microsatellite genotype 1. Only 1 Mexican strain resistant to both drugs was found in this study.

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Section: Discussionmentioning

confidence: 99%

“…Subgroups of TcI have also been reported, using mini-exon gene sequences and microsatellite analysis (Macedo et al 2001, Falla et al 2009, Llewelyn et al 2009, Ocañ a-Mayorga et al 2010, Barnabé et al 2011). …”

Section: Introductionmentioning

confidence: 96%

Microsatellite and Mini-Exon Analysis of Mexican Human DTU ITrypanosoma cruziStrains and Their Susceptibility to Nifurtimox and Benznidazole

Martı́nez

Nogueda

Martínez‐Hernández

et al. 2013

Vector-Borne and Zoonotic Diseases

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“…Both hypotheses find support in the literature (Nirdé et al 1995, Veloso et al 2001, Villarreal et al 2005, Wilkinson et al 2008). Due to the remarkable polymorphism of microsatellite markers, as few as four microsatellite loci is usually enough to differentiate any two T. cruzi populations, even those that are genetically closely related (Oliveira et al 1998, Macedo et al 2001. Based on the profile of nine microsatellite loci, we ruled out any parasite selection process that may have occurred during or after BZ treatment.…”

Section: Discussionmentioning

confidence: 99%

Trypanosoma cruzi benznidazole susceptibility in vitro does not predict the therapeutic outcome of human Chagas disease

Moreno

D’Ávila²,

Silva

et al. 2010

Mem. Inst. Oswaldo Cruz

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“…Trypanosoma cruzi exhibits a high degree of intraspecific variability, as detected by biological, biochemical, immunological, and genetic markers 17 . Based on different molecular markers, the Second Satellite Meeting recommended that T. cruzi should be classified into six discrete typing units (DTUs; T. cruzi I-VI) 18 .…”

Section: Introductionmentioning

confidence: 99%

Combined parasitological and molecular-based diagnostic tools improve the detection of Trypanosoma cruzi in single peripheral blood samples from patients with Chagas disease

Volpato

Sousa

D’Ávila

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction:In order to detect Trypanosoma cruzi and determine the genetic profiles of the parasite during the chronic phase of Chagas disease (ChD), parasitological and molecular diagnostic methods were used to assess the blood of 91 patients without specific prior treatment. Methods: Blood samples were collected from 68 patients with cardiac ChD and 23 patients with an indeterminate form of ChD, followed by evaluation using blood culture and polymerase chain reaction. T. cruzi isolates were genotyped using three different genetic markers. Results: Blood culture was positive in 54.9% of all patients, among which 60.3% had the cardiac form of ChD, and 39.1% the indeterminate form of ChD. There were no significant differences in blood culture positivity among patients with cardiac and indeterminate forms. Additionally, patient age and clinical forms did not influence blood culture results. Polymerase chain reaction (PCR) was positive in 98.9% of patients, although comparisons between blood culture and PCR results showed that the two techniques did not agree. Forty-two T. cruzi stocks were isolated, and TcII was detected in 95.2% of isolates. Additionally, one isolate corresponded to TcIII or TcIV, and another corresponded to TcV or TcVI. Conclusions: Blood culture and PCR were both effective for identifying T. cruzi using a single blood sample, and their association did not improve parasite detection. However, we were not able to establish an association between the clinical form of ChD and the genetic profile of the parasite.

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Usefulness of microsatellite typing in population genetic studies of Trypanosoma cruzi

Cited by 59 publications

References 13 publications

Microsatellite and Mini-Exon Analysis of Mexican Human DTU ITrypanosoma cruziStrains and Their Susceptibility to Nifurtimox and Benznidazole

Microsatellite and Mini-Exon Analysis of Mexican Human DTU ITrypanosoma cruziStrains and Their Susceptibility to Nifurtimox and Benznidazole

Trypanosoma cruzi benznidazole susceptibility in vitro does not predict the therapeutic outcome of human Chagas disease

Combined parasitological and molecular-based diagnostic tools improve the detection of Trypanosoma cruzi in single peripheral blood samples from patients with Chagas disease

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