Usefulness of melanoma antigen (MAGE) gene analysis in tissue samples from percutaneous needle aspiration biopsy of suspected lung cancer lesions

Kim, Hana; Kim, Sang Jin; Lee, Sun-Hwa; Ra, Seong H; KyungOk, Lee; Jeon, Chang‐Ho; Hong, Yoo Jin; Lee, Sang Min; Kim, Tae Hoon

doi:10.1016/j.lungcan.2009.12.003

Cited by 8 publications

(12 citation statements)

References 17 publications

(20 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, MAGE positive benign lung diseases were mainly comprised of pulmonary tuberculosis and inflammatory diseases and severity of inflammation was not evaluated. Kim et al (15) reported MAGE expression in tissue samples obtained using a percutaneous needle aspiration biopsy of tuberculosis patients. We performed MAGE A1-A6 nested PCR using DNA of Mycobacterium tuberculosis ; however, the MAGE gene was not amplified.…”

Section: Discussionmentioning

confidence: 99%

MAGE A1-A6 RT-PCR and MAGE A3 and p16 methylation analysis in induced sputum from patients with lung cancer and non-malignant lung diseases

Shin

Lee²,

Lee³

et al. 2011

Oncology Reports

Self Cite

View full text Add to dashboard Cite

The melanoma antigen gene (MAGE) A1-A6 RT-PCR system was developed for the detection of lung cancer cells in the sputum. However, we identified MAGE expression in some patients with non-malignant lung diseases. To understand these patterns of MAGE expression, we performed MAGE A3 methylation-specific PCR (MSP) and p16 MSP. We collected 24 biopsy specimens of lung cancer tissue and performed MAGE A1-A6 RT-PCR, MAGE A3 MSP and p16 MSP. RNA and DNA were simultaneously extracted from induced sputum specimens of 133 patients with lung diseases and 30 random sputum specimens of healthy individuals and the 3 molecular analyses were performed. The patients were diagnosed as 65 cases of lung cancer and 68 of benign lung diseases. Positive rates of MAGE A1-A6 RT-PCR, MAGE A3 MSP and p16 MSP were as follows: in lung cancer tissue, 87.5, 58.3 and 70.8%; in the sputum of lung cancer patients, 50.8, 46.2 and 63.1%; benign lung diseases, 10.3, 30.9 and 39.7%; and healthy individuals, 3.3, 6.7 and 3.3%. Of the 40 MAGE-positive cases, 33 were diagnosed with lung cancer and 7 as having benign lung diseases. From the 7 cases of MAGE-positive benign lung diseases, 6 cases showed methylation abnormalities. The MAGE-positive group revealed significantly higher rates of methylation abnormalities. Of the 40 MAGE-positive cases, 39 cases were found to be lung cancer or benign lung diseases with abnormal methylation. Thus, MAGE expression in the sputum suggests the presence of lung cancer cells or pre-cancerous cells.

show abstract

Section: Discussionmentioning

confidence: 99%

MAGE A1-A6 RT-PCR and MAGE A3 and p16 methylation analysis in induced sputum from patients with lung cancer and non-malignant lung diseases

Shin

Lee²,

Lee³

et al. 2011

Oncology Reports

Self Cite

View full text Add to dashboard Cite

show abstract

“…The melanoma antigen gene (MAGE) family is a group of cancer testis antigens, which are isolated from melanoma cells [4]. MAGE antigens are not expressed in normal tissues other than the testis.…”

Section: Introductionmentioning

confidence: 99%

Pilot Study on MAGE-C2 as a Potential Biomarker for Triple-Negative Breast Cancer

Zhao

Shalieer

2016

Disease Markers

View full text Add to dashboard Cite

Objective. In the current study, we measured the expression status of melanoma antigen gene c2 (MAGE-C2) in triple-negative breast cancer (TNBC) and analyzed its prognostic with the clinical pathological features of patients with TNBC. Methods. The expressions statuses of MAGE-C2 were detected in TNBC tissues and paracarcinoma tissues by immunohistochemistry, reverse transcription-polymerase chain reaction (RT-PCR), and western blotting. Then, we investigated the relationship of MAGE-C2 expression status and clinicopathological parameters of TNBC patients by the chi-squared test. Finally, we discussed the relations of MAGE-C2 expression state and prognosis of patients with TNBC by Kaplan-Meier method and Cox proportional hazards model. Results. High MAGE-C2 expression was found in 38.18% (42/110) of TNBC tissues. In adjacent tissues it was 9.09% (10/110). High MAGE-C2 expression in TNBC patients was closely associated with lymph node status, tumor node metastasis (TNM) stage, and lymphovascular invasion (P < 0.001). TNBC patients with high MAGE-C2 expression had significantly shorter survival time than low expression patients. We also found that age, lymph node status, TNM stage, lymphovascular invasion, and MAGE-C2 expression status were closely associated with overall survival of TNBC patients (P < 0.05). Conclusion. High MAGE-C2 expression may serve as an independent prognostic factor for TNBC patients.

show abstract

“…In previous studies, the diagnostic sensitivity of MAGE RT‐nested PCR differed in relation to the lung tumor histology, 14,19 with the diagnostic sensitivity of MAGE RT‐nested PCR in the sputum, bronchial washing fluid, and pleural fluid, higher in patients with squamous cell carcinoma than in those with adenocarcinoma 19 . The diagnostic sensitivity of MAGE RT‐nested PCR on tissue samples from percutaneous needle aspiration was 100% in patients with squamous cell carcinoma and 80% in patients with adenocarcinoma 14 . In this study, lung adenocarcinoma was the most common histology of malignant pleural effusion from primary lung cancer (80.0%), with lung squamous cell carcinoma present in only 5.7% of those with malignant pleural effusion from primary lung cancer.…”

Section: Discussionmentioning

confidence: 91%

“…MAGE genes are expressed only in tumor cells, the male reproductive organs, and the placenta; there is no expression in other normal tissues 8–12 . MAGE RT‐nested PCR with MAGE A1‐6 common primers has been found to be useful in patients with primary lung cancer 13,14,19 . However, the role of MAGE RT‐nested PCR in combination with other diagnostic modalities was not reported.…”

Section: Discussionmentioning

confidence: 99%

“…MAGE is expressed in many kinds of malignancies, including lung cancer, stomach cancer, ovarian cancer, and leukemia 9–12 . The use of MAGE reverse transcriptase‐nested polymerase chain reaction (RT‐nested PCR) in the diagnosis of lung cancer, using specimens from bronchial washing and percutaneous needle aspiration biopsy, has been reported 13,14 . However, the role of MAGE RT‐nested PCR in the diagnosis of malignant pleural effusion is not well understood.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The role of pleural fluid MAGE RT‐nested PCR in the diagnosis of malignant pleural effusion

et al. 2012

View full text Add to dashboard Cite

Background: Melanoma antigen (MAGE) genes are expressed in tumor cells, the testis and the placenta. The purpose of this prospective study was to investigate the sensitivity, specificity, and accuracy of the carcinoembryonic antigen (CEA), MAGE reverse transcriptase-nested polymerase chain reaction (RT-nested PCR), and cytology of pleural fluid in the diagnosis of malignant pleural effusion. Methods: Patients in whom unilateral pleural effusion was identified on chest radiography from January to December 2009 were included in the study. MAGE genes were analyzed by RT-nested PCR using MAGE A1-6 common primers. Results: Of 81 enrolled patients, 46 were diagnosed as malignant pleural effusion, and 24 were diagnosed as benign pleural effusion. The diagnoses of 11 patients were not confirmed in this study. The diagnostic sensitivity, specificity, and accuracy of MAGE RT-nested PCR were 61.4%, 95.7%, and 73.1%, respectively. The diagnostic sensitivities of cytology and CEA (>5 ng/mL) were 61.4% and 75.0%, respectively. Among 17 patients with negative cytology who had malignant pleural effusion, 12 and 10 patients were positive for CEA (>5.0 ng/mL) and MAGE RT-nested PCR, respectively. However, of five patients with malignant pleural effusion that was not recognized by cytology and CEA, MAGE RT-nested PCR correctly predicted a malignant etiology in only one additional patient (20%). Conclusions: MAGE RT-nested PCR seems to add little on the combination of conventional methods in the diagnosis of malignant effusion.

show abstract

Usefulness of melanoma antigen (MAGE) gene analysis in tissue samples from percutaneous needle aspiration biopsy of suspected lung cancer lesions

Cited by 8 publications

References 17 publications

MAGE A1-A6 RT-PCR and MAGE A3 and p16 methylation analysis in induced sputum from patients with lung cancer and non-malignant lung diseases

MAGE A1-A6 RT-PCR and MAGE A3 and p16 methylation analysis in induced sputum from patients with lung cancer and non-malignant lung diseases

Pilot Study on MAGE-C2 as a Potential Biomarker for Triple-Negative Breast Cancer

The role of pleural fluid MAGE RT‐nested PCR in the diagnosis of malignant pleural effusion

Contact Info

Product

Resources

About