Usefulness of Measuring Serum Amyloid A Concentration in Japanese Black Cattle in Clinical Practice

Shinya, Urara; Yamato, Osamu; Iwamura, Yuka; Kato, Tomohiro; Hamada, Yuhei; Widodo, Oky Setyo; Taniguchi, Masayasu; Takagi, Mitsuhiro

doi:10.3390/vetsci10080528

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…Measured concentrations of plasma haptoglobin and SAA concentrations in this study were in part much higher than reported in previous field studies on the diagnostic utility of these biomarkers ( 24 , 30 , 31 , 56–59 ). This may partially be due to the inclusion of a highly preselected study population of second-opinion cases referred to a university teaching hospital, which may create a bias toward more severely affected cases.…”

Section: Discussioncontrasting

confidence: 80%

Association of results of the glutaraldehyde coagulation test with plasma acute phase protein concentrations and hematologic findings in hospitalized cows

Trefz,

Balmer,

Peters

et al. 2024

Front. Vet. Sci.

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IntroductionThe glutaraldehyde test (GAT) allows for animal-side semi-quantitative estimation of fibrinogen and gamma-globulin concentrations in blood samples of adult cattle and therefore detection of inflammatory disease conditions. However, the test has potential limitations, especially due to the latency period until sufficiently high fibrinogen and/or gamma-globulin concentrations are reached. The aim of the present study was therefore to assess the association between results of GAT with other inflammatory markers including hematologic variables, fibrinogen, plasma haptoglobin and serum amyloid A (SAA) concentrations.MethodsFor the purpose of this prospective observational study, a convenience sample of 202 cows with a broad range of inflammatory and non-inflammatory clinical conditions was included. The GAT was run on EDTA blood, fibrinogen was measured using the Clauss and the heat precipitation method, and commercially available ELISA tests were used for determination of plasma haptoglobin and SAA concentrations.ResultsShortened GAT coagulation times were more closely correlated to serum globulin (rs = −0.72) than to plasma fibrinogen concentrations measured with the heat precipitation (rs = −0.64) and the Clauss method (rs = −0.70). Cows with a markedly (≤3 min) or moderately (4–6 min) shortened coagulation time had higher (p < 0.001) plasma haptoglobin and SAA concentrations than cows with a negative test result. Total leukocyte, monocyte and neutrophil concentrations did not differ significantly between groups. An identified cut-off for the GAT coagulation time of ≤14 min had a sensitivity and specificity of 54.4 and 100%, respectively, for the prediction of an inflammatory state based on clinical findings and/or increased plasma haptoglobin or SAA concentrations.DiscussionIn conclusion, this study demonstrates considerable diagnostic agreement between positive GAT results and increased plasma concentrations of haptoglobin and SAA. Despite high specificity, the test lacks sensitivity in case of acute inflammatory conditions indicating that plasma acute phase protein concentrations and hematologic findings can provide additional diagnostic information if the GAT is negative.

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Section: Discussioncontrasting

confidence: 80%

Association of results of the glutaraldehyde coagulation test with plasma acute phase protein concentrations and hematologic findings in hospitalized cows

Trefz,

Balmer,

Peters

et al. 2024

Front. Vet. Sci.

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show abstract

“…Measured concentrations of plasma haptoglobin and SAA concentrations in this study were in part much higher than reported in previous field studies on the diagnostic utility of these biomarkers (24, 30,31,[56][57][58][59]. This may partially be due to the inclusion of a highly preselected study population of second-opinion cases referred to a university teaching hospital, which may create a bias toward more severely affected cases.…”

Section: Discussioncontrasting

confidence: 68%

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Performance of an automated immunoturbidimetric assay for bovine serum amyloid A

Nielsen,

Petersen,

Capion

et al. 2024

Veterinary Clinical Pathol

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BackgroundAcute phase proteins are a group of vital constituents of the innate immune system, which may also serve as circulatory biomarkers of inflammation. The major acute phase protein serum amyloid A (SAA) is a reliable and sensitive biomarker in cows, allowing for rapid detection of inflammatory disease. A multispecies automated immunoturbidimetric assay (VET‐SAA, Eiken) has been validated for horses, dogs, and cats, and it has been used to measure SAA concentrations in bovine samples.ObjectivesThe aim of the present study was to perform an analytical validation of the VET‐SAA immunoturbidometric assay based on monoclonal antihuman SAA antibodies for the measurement of SAA in clinical samples from cows.Methods and ResultsThe validation included an assessment of imprecision, inaccuracy, and detection limit, as well as an evaluation of the overlap performance, using banked serum from healthy and sick cows with or without inflammatory disease. Intra‐ and interassay variation ranged from 0.91% to 2.9% and 2.5% to 5.8%, respectively. The assay was performed with acceptable accuracy within a clinically relevant range of SAA, although minor signs of inaccuracy were detected. Overlap performance was acceptable, with the VET‐SAA assay able to differentiate between healthy cows and cows with inflammatory and noninflammatory conditions. The automated VET‐SAA assay is considered acceptable for the measurement of SAA in cows.

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Usefulness of Measuring Serum Amyloid A Concentration in Japanese Black Cattle in Clinical Practice

Cited by 3 publications

References 29 publications

Association of results of the glutaraldehyde coagulation test with plasma acute phase protein concentrations and hematologic findings in hospitalized cows

Association of results of the glutaraldehyde coagulation test with plasma acute phase protein concentrations and hematologic findings in hospitalized cows

Table_1.DOCX

Performance of an automated immunoturbidimetric assay for bovine serum amyloid A

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