Usefulness of Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry in the Characterization of
            <i>Leishmania</i>
            Strains Causing Tegumentary Leishmaniasis in Bolivia versus
            <i>hsp70</i>
            Gene Sequencing

Torrico, Mary Cruz; Fernández-Arévalo, Anna; Ballart, Cristina; Solano, Marco; Rojas, Ernesto; Abràs, Alba; Gonzales, Fabiola; Arnau, Albert; Tebar, Sílvia; Llovet, Teresa; Lozano, Daniel; Ariza-Vioque, Eva; Gascón, Joaquím; Picado, Albert; Torrico, Faustino; Muñoz, Carmen; Gállego, Montserrat

doi:10.1128/spectrum.03477-22

Cited by 2 publications

(1 citation statement)

References 39 publications

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“…The MALDI-TOF MS can also identify the unknown bacteria by the masses of peptides of the unknown organisms’ biomarkers ( Singhal et al., 2015 ). This technology can identify microorganism including bacterium, fungus, and parasites ( Suranyi et al., 2023 ; Torrico et al., 2023 ). In this research, we used MALDI-TOF MS technique to identify single colonies isolated from masks worn for 4 and 5 hours.…”

Section: Introductionmentioning

confidence: 99%

Bacterial contamination of medical face mask wearing duration and the optimal wearing time

Ding,

Li,

Liu

et al. 2023

Front. Cell. Infect. Microbiol.

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IntroductionBacterial contamination is a critical parameter for how long a medical mask will be worn.MethodsIn this study, we used the pour plate method to observe the total bacteria counts in used medical face masks. The bacterial community analysis was detected using bio-Mass spectrometry technology and 16SrRNA gene sequencing technology. The wearing time of the mask from 0.5 hours to 5 hours were studied.ResultsThese results shown that the total number of bacteria on the inside surface of the mask were higher than the outside. The total number of bacteria on the inner surface of masks worn for 0.5 h, 1 h 2 h, 4 h and 5 h was 69 CFU/m2,91.3 CFU/m2, 159.6 CFU/m2, 219 CFU/m2, and 879 CFU/m2, respectively. The total number of bacteria on the outside surface of masks worn for 0.5 h, 1 h 2 h, 4 h and 5 h was 60 CFU/m2, 82.7 CFU/m2, 119.8 CFU/m2, 200 CFU/m2, and 498 CFU/m2, respectively. The bacterial abundance obtained from bio-Mass spectrometry were consistent with the results of 16SrRNA sequencing. Both the methods discovered the maximum number of Neisseria followed by Corynebacterium species in mask worn 5 hours. The top 100 bacteria isolated from inside and outside surface of mask belong to 11 phyla.ConclusionsWe analyzed bacterial penetration efficiency of the bacteria that were detected both on the inside and outside surface of the masks. In the top 10 bacteria, no bacteria were detected both inside and outside the mask worn for four hours, while 6 bacteria species were detected on the inside and outside of the mask after wearing for five hours. Bacterial penetration rates ranged from 0.74% to 99.66% for masks worn continuously for five hours, and the penetration rate of four strains exceeded 10% in the top 10 colonies. We recommend timely replacement of masks worn for more than four hours.

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Section: Introductionmentioning

confidence: 99%