Usefulness of IS -restriction fragment length polymorphism typing of Brazilian strains of   and comparison with an international fingerprint database

Suffys, Philip Noël; Araujo, Marcelo Emanuel Ivens de; Rossetti, Maria Lúcia Rosa; Zaha, Arnaldo; Barroso, Eduardo Werneck; Barreto, Angela Maria Werneck; Campos, Eduardo; Soolingen, Dick van; Kremer, Kristien; Heersma, Herre; Degrave, Wim

doi:10.1016/s0923-2508(00)00157-1

Cited by 23 publications

(23 citation statements)

References 39 publications

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“…The value of the IS6110-RFLP as an additional tool for epidemological studies of TB in Brazil has already been demonstrated (Suffys et al 2000) and both IS6110 copy number and genetic polymorphism of the strains collected in the present study were high, confirming our earlier data. A clustering rate of 26.3% was observed, similar to the rate observed in hospitals and in the general population (Ivens de Araújo et al 1998, Ferrazoli et al 2000, Lourenço et al 2000.…”

Section: Discussionsupporting

confidence: 92%

Drug resistance and genotypes of strains of Mycobacterium tuberculosis isolated from human immunodeficiency virus-infected and non-infected tuberculosis patients in Bauru, São Paulo, Brazil

Baptista

Oelemann

Opromolla

et al. 2002

Mem. Inst. Oswaldo Cruz

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Section: Discussionsupporting

confidence: 92%

Drug resistance and genotypes of strains of Mycobacterium tuberculosis isolated from human immunodeficiency virus-infected and non-infected tuberculosis patients in Bauru, São Paulo, Brazil

Baptista

Oelemann

Opromolla

et al. 2002

Mem. Inst. Oswaldo Cruz

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“…In the last years, considerable efforts have been made to precise the epidemiology and characteristics of M. tuberculosis strains isolated in different regions of Brazil [7,[24][25][26][27][28][29][30][31]. Some Brazilian authors [26,27,29,32] demonstrated the usefulness of the IS6110-restriction fragment length polymorphism for typing Brazilian M. tuberculosis strains, but Ferreira et al [24] were the first to apply it to determine strain types in female inmate populations in São Paulo City, Brazil.…”

Section: Discussionmentioning

confidence: 99%

“…Some Brazilian authors [26,27,29,32] demonstrated the usefulness of the IS6110-restriction fragment length polymorphism for typing Brazilian M. tuberculosis strains, but Ferreira et al [24] were the first to apply it to determine strain types in female inmate populations in São Paulo City, Brazil. Ferreira et al [24] suggested that in 50% inmates infection was a reactivation process of a previous latent infection, while in the 50%, TB was transmitted within prison (exogenous infection).…”

Section: Discussionmentioning

confidence: 99%

Molecular genotyping and epidemiology of Mycobacterium tuberculosis isolates obtained from inmates of correctional institutions of Campinas, Southeast Brazil

Moreira-Oliveira¹,

Oliveira²,

Pace³

et al. 2008

Braz J Infect Dis

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The objective of this study was to investigate the possible transmission of tuberculosis among 39 inmates with positive Mycobacterium tuberculosis smears in four correctional institutions located in Campinas City, SP, Brazil over a 19-month period. Fifty-one M. tuberculosis isolates from these inmates were characterized according to the number of IS6110 insertion elements present in their genomic DNA. The number of insertion elements in M. tuberculosis isolates varied from two to twelve. The dendrogram of similarity resulted in the grouping the isolates in six main clusters. These results, associated to epidemiological data, suggested the transmission of tuberculosis among inmates of the same and different institutions inmates. Univariate analysis of epidemiological data (total delay for beginning of treatment, previous treatment, and HIV status) and clustering occurrence showed that only "previous treatment" (OR = 7.65, p = 0.032) was associated with the possible transmission of tuberculosis in the studied prisons.

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“…The rates of infection were as follows: 23% and 37% (between 1991 and 1994) in the states of Rio de Janeiro and Rio Grande do Sul, respectively (27) ; 33% (between 1995 and 1997) in the city of São Paulo (28) ; and 29% (between 2000 and 2002) in the city of Araraquara.…”

Section: Discussionmentioning

confidence: 99%

Ocorrência de tuberculose em um hospital psiquiátrico do interior de Goiás

et al. 2006

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Objective: To investigate the prevalence of infection, disease and eventual institutional outbreak of tuberculosis in a psychiatric hospital using the PPD test, as well as testing for mycobacteria in material collected from the bronchial tree and using molecular tracking technique based on insertion sequence 6110 (IS6110). Methods: Between February and August of 2002, PPD tests were given to 74 inpatients and 31 staff members at a psychiatric hospital in the city of Rio Verde, located in the state of Goiás, Brazil. In addition, bronchial tree material collected from the inpatients was submitted to testing for Mycobacterium tuberculosis. Results: Among the patients analyzed, mycobacteria were isolated from five (6.8%): four identified as M. tuberculosis and one as M. chelonae. The M. tuberculosis isolates were sensitive to isoniazid and rifampicin, and, when submitted to the restriction fragment length polymorphism/IS6110 technique, presented unique genetic profiles, totally distinct from one another, suggesting that all of the tuberculosis cases were due to endogenous reactivation. It was not possible to characterize this group of cases as an institutional outbreak. Performing the two-step tuberculin test in the patients, the infection rates were 23% and 31%, compared with 42% among staff members, who were submitted to the one-step test. Conclusion: The results indicate a high incidence of tuberculosis infection among inpatients and hospital staff, as well as a high occurrence of the disease among inpatients.

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Usefulness of IS -restriction fragment length polymorphism typing of Brazilian strains of and comparison with an international fingerprint database

Cited by 23 publications

References 39 publications

Drug resistance and genotypes of strains of Mycobacterium tuberculosis isolated from human immunodeficiency virus-infected and non-infected tuberculosis patients in Bauru, São Paulo, Brazil

Drug resistance and genotypes of strains of Mycobacterium tuberculosis isolated from human immunodeficiency virus-infected and non-infected tuberculosis patients in Bauru, São Paulo, Brazil

Molecular genotyping and epidemiology of Mycobacterium tuberculosis isolates obtained from inmates of correctional institutions of Campinas, Southeast Brazil

Ocorrência de tuberculose em um hospital psiquiátrico do interior de Goiás

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