Usefulness of Intravital Multiphoton Microscopy in Visualizing Study of Mouse Cochlea and Volume Changes in the Scala Media

Ju, Hyun Mi; Lee, Sun Hee; Kong, Tae Hoon; Kwon, Seung‐Hae; Choi, Jin Woo; Seo, Young Joon

doi:10.3389/fneur.2017.00332

Cited by 6 publications

(3 citation statements)

References 28 publications

(31 reference statements)

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“…Others have previously used SHG and TPEF imaging to investigate pathological changes to the cochlea. In one such study, intravital multiphoton microscopy was used to measure the volume of the scala media compartment in the apical turns of control and pendrin-deficient mice 37 . In another, little difference was observed between the SHG signals detected from the SG and basilar membranes of control and noise-exposed cochleae 21 .…”

Section: Discussionmentioning

confidence: 99%

Chronic cigarette smoke exposure drives spiral ganglion neuron loss in mice

Paquette

Dawes

Sundar

et al. 2018

Sci Rep

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Tobacco use is associated with an increased risk of hearing loss in older individuals, suggesting cigarette smoke (CS) exposure may target the peripheral auditory organs. However, the effects of CS exposure on general cochlear anatomy have not previously been explored. Here we compare control and chronic CS exposed cochleae from adult mice to assess changes in structure and cell survival. Two-photon imaging techniques, including the imaging of second harmonic generation (SHG) and two-photon excitation fluorescence (TPEF) from native molecules, were used to probe the whole cochlear organ for changes. We found evidence for fibrillar collagen accumulation in the spiral ganglion and organ of Corti, consistent with fibrosis. Quantitative TPEF indicated that basal CS-exposed spiral ganglion neurons experienced greater oxidative stress than control neurons, which was confirmed by histological staining for lipid peroxidation products. Cell counts confirmed that the CS-exposed spiral ganglion also contained fewer basal neurons. Taken together, these data support the premise that CS exposure induces oxidative stress in cochlear cells. They also indicate that two-photon techniques may screen cochlear tissues for oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Chronic cigarette smoke exposure drives spiral ganglion neuron loss in mice

Paquette

Dawes

Sundar

et al. 2018

Sci Rep

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“…Cochlear dissection and tissue preparation for cryosection were performed as previously described with some modifications. 20 The cochlea were immersed directly in 4% formaldehyde (Biosesang Seongnam, South Korea) for 24 h at room temperature, decalcified by immersion in 0.5 M EDTA for 48 h, dehydrated in 30% sucrose (Sigma-Aldrich, Gillingham, UK) for 24 h, embedded in optimal cutting temperature compound (Leica, Bensheim, Germany), and sectioned at 4 to 5 μm thickness in a cryostat (Leica CM1850 Cryostat; Leica, Wetzlar, Germany). The slices were mounted on slides and stained (eosin for cochlear structures and Prussian blue for PSD-NPs).…”

Section: Methodsmentioning

confidence: 99%

Protection of Hearing Loss in Ototoxic Mouse Model Through SPIONs and Dexamethasone-Loaded PLGA Nanoparticle Delivery by Magnetic Attraction

Park¹,

Kim²,

Kim³

et al. 2022

IJN

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Background Ototoxicity currently has no available treatment other than medication withdrawal as soon as toxicity is suspected. The human inner ear organs have little potential for regeneration; thus, ototoxicity-induced hair cell injury is deemed permanent. Dexamethasone (Dexa) is a synthetic steroid analog that has significant potential for otoprotection in the treatment of various inner ear diseases; however, its low absorption into the inner ear prevents significant recovery of function. Nanoparticles facilitate targeted drug delivery, stabilize drug release, and increase half-life of the drug. Methods This study aimed to develop poly(lactic-co-glycolic acid) (PLGA) nanoparticles loaded superparamagnetic iron oxide nanoparticles (SPIONs) and Dexa (PSD-NPs) to control localized drug delivery by magnetic attraction in the treatment of ototoxicity-induced hearing loss. PSD-NPs and without SPIONs (PD-NPs) were prepared using a nanoprecipitation method. Results Using an inner ear simulating system, we confirmed that PSD-NPs has an otoprotective effect in organotypic culture that is enhanced by magnetic attraction. PSD-NPs delivered via intrabullar injection in a magnetic field penetrated the inner ear and prevented hearing loss progression to a greater degree than equivalent doses of Dexa or PSD-NPs alone (day 28: ototoxic: 80.0 ± 0.0 dB; Dexa 100: 60.0 ± 15.5 dB; PSD 100: 50.0 ± 8.2 dB; PSD 100 with magnet: 22.5 ± 5.0 dB; P < 0.05). The protective effects were confirmed in various in vivo and in vitro models of ototoxicity. Conclusion Our findings suggest that SPIONs with Dexa and magnetic field application prevent the progression of ototoxicity-induced hearing loss through anti-apoptotic mechanisms in the inner ear.

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“…Intravital microscopy (IVM) has emerged as a profound imaging modality for gaining insight into dynamic processes on the cellular and subcellular level, particularly for visualizing nanoparticles [ 14 , 18 , 19 , 20 , 21 ]. A primary challenge in the clinical translation of nanoparticles is identifying and understanding their biological fate, which relies on the visualization of these processes.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Visualization and Tracking of Gold Nanoparticles in Vasculature Using Multiphoton Luminescence

et al. 2020

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Gold nanoparticles continue to generate interest for use in several biomedical applications. Recently, researchers have been focusing on exploiting their dual diagnostic/therapeutic theranostic capabilities. Before clinical translation can occur, regulatory agencies will require a greater understanding of their biodistribution and safety profiles post administration. Previously, the real-time identification and tracking of gold nanoparticles in free-flowing vasculature had not been possible without extrinsic labels such as fluorophores. Here, we present a label-free imaging approach to examine gold nanoparticle (AuNP) activity within the vasculature by utilizing multiphoton intravital microscopy. This method employs a commercially available multiphoton microscopy system to visualize the intrinsic luminescent signal produced by a multiphoton absorption-induced luminescence effect observed in single gold nanoparticles at frame rates necessary for capturing real-time blood flow. This is the first demonstration of visualizing unlabeled gold nanoparticles in an unperturbed vascular environment with frame rates fast enough to achieve particle tracking. Nanoparticle blood concentration curves were also evaluated by the tracking of gold nanoparticle flow in vasculature and verified against known pre-injection concentrations. Half-lives of these gold nanoparticle injections ranged between 67 and 140 s. This label-free imaging approach could provide important structural and functional information in real time to aid in the development and effective analysis of new metallic nanoparticles for various clinical applications in an unperturbed environment, while providing further insight into their complex uptake and clearance pathways.

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Usefulness of Intravital Multiphoton Microscopy in Visualizing Study of Mouse Cochlea and Volume Changes in the Scala Media

Cited by 6 publications

References 28 publications

Chronic cigarette smoke exposure drives spiral ganglion neuron loss in mice

Chronic cigarette smoke exposure drives spiral ganglion neuron loss in mice

Protection of Hearing Loss in Ototoxic Mouse Model Through SPIONs and Dexamethasone-Loaded PLGA Nanoparticle Delivery by Magnetic Attraction

Label-Free Visualization and Tracking of Gold Nanoparticles in Vasculature Using Multiphoton Luminescence

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