2017
DOI: 10.3389/fneur.2017.00332
|View full text |Cite
|
Sign up to set email alerts
|

Usefulness of Intravital Multiphoton Microscopy in Visualizing Study of Mouse Cochlea and Volume Changes in the Scala Media

Abstract: Conventional microscopy has limitations in viewing the cochlear microstructures due to three-dimensional spiral structure and the overlying bone. But these issues can be overcome by imaging the cochlea in vitro with intravital multiphoton microscopy (MPM). By using near-infrared lasers for multiphoton excitation, intravital MPM can detect endogenous fluorescence and second harmonic generation of tissues. In this study, we used intravital MPM to visualize various cochlear microstructures without any staining an… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

0
3
0

Year Published

2017
2017
2022
2022

Publication Types

Select...
5

Relationship

2
3

Authors

Journals

citations
Cited by 6 publications
(3 citation statements)
references
References 28 publications
(31 reference statements)
0
3
0
Order By: Relevance
“…Others have previously used SHG and TPEF imaging to investigate pathological changes to the cochlea. In one such study, intravital multiphoton microscopy was used to measure the volume of the scala media compartment in the apical turns of control and pendrin-deficient mice 37 . In another, little difference was observed between the SHG signals detected from the SG and basilar membranes of control and noise-exposed cochleae 21 .…”
Section: Discussionmentioning
confidence: 99%
“…Others have previously used SHG and TPEF imaging to investigate pathological changes to the cochlea. In one such study, intravital multiphoton microscopy was used to measure the volume of the scala media compartment in the apical turns of control and pendrin-deficient mice 37 . In another, little difference was observed between the SHG signals detected from the SG and basilar membranes of control and noise-exposed cochleae 21 .…”
Section: Discussionmentioning
confidence: 99%
“…Cochlear dissection and tissue preparation for cryosection were performed as previously described with some modifications. 20 The cochlea were immersed directly in 4% formaldehyde (Biosesang Seongnam, South Korea) for 24 h at room temperature, decalcified by immersion in 0.5 M EDTA for 48 h, dehydrated in 30% sucrose (Sigma-Aldrich, Gillingham, UK) for 24 h, embedded in optimal cutting temperature compound (Leica, Bensheim, Germany), and sectioned at 4 to 5 μm thickness in a cryostat (Leica CM1850 Cryostat; Leica, Wetzlar, Germany). The slices were mounted on slides and stained (eosin for cochlear structures and Prussian blue for PSD-NPs).…”
Section: Methodsmentioning
confidence: 99%
“…Intravital microscopy (IVM) has emerged as a profound imaging modality for gaining insight into dynamic processes on the cellular and subcellular level, particularly for visualizing nanoparticles [ 14 , 18 , 19 , 20 , 21 ]. A primary challenge in the clinical translation of nanoparticles is identifying and understanding their biological fate, which relies on the visualization of these processes.…”
Section: Introductionmentioning
confidence: 99%