Usefulness of hard X‐ray microscope using synchrotron radiation for the structure analysis of insects

Lee, Jiwon; Lee, Onseok

doi:10.1002/jemt.22978

Cited by 12 publications

(20 citation statements)

References 27 publications

(26 reference statements)

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“…In recent years, the application of X-ray Microscopy in the Life Sciences is becoming an established imaging technique. This technology has been applied in insect taxonomy, paleontology and other Life Science focused research fields [27, 28]. .…”

Section: Discussionmentioning

confidence: 99%

The microstructure investigation of plant architecture with X-ray microscopy

Zhang

Guo

Chen

et al. 2019

Preprint

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18Background 19 In recent years, the plant morphology has been well studied by multiple approaches at 20 cellular and subcellular levels. Two-dimensional (2D) microscopy techniques offer 21 imaging of plant structures on a wide range of magnifications for researchers. However, 22 subcellular imaging is still challenging in plant tissues like roots and seeds. 23 Results 24Here we use a three-dimensional (3D) imaging technology based on the ZEISS X-ray 25 microscope (XRM) Versa and analyze several plant tissues from different plant species. 26 The XRM provides new insights into plant structures using non-destructive imaging at 27 high-resolution and high contrast. We also developed a workflow aiming to acquire 28 accurate and high-quality images in the context of the whole specimen. Multiple plant 29 samples including rice, tobacco, Arabidopsis and maize were used to display the 30 differences of phenotypes, which indicates that the XRM is a powerful tool to 31 investigate plant microstructure. 32 Conclusions 33 Our work provides a novel observation method to evaluate and quantify tissue specific 34 differences for a range of plant species. This new tool is suitable for non-destructive 35 seed observation and screening. 36 37 Keywords: X-ray microscopy, plant morphology, plant development, plant 3D 38 reconstruction 39 Background 40 Since the invention and development of microscopes, it has extended human vision 41 substantially. The observation of cellular and subcellular structures using microscopes 42 have broadened our knowledge to understand the biological world more efficiently [1]. 43 ZEISS and many other microscopy manufacturing companies are spending an 44 enormous amount of time and resources developing higher resolution microscopy 45 systems to assist scientists acquire more detailed images in their research fields. From 46 the single cell organism blue-green algae (Cyanobacteria) to over a hundred-meter-tall 47 giant tree (Eucalyptus regnans), plants display versatile morphologies to survive in 48 different environments. Therefore, utilizing microscopy techniques to study the cellular 49 and subcellular and physiological traits is essential in plant research.50 51 In the 21 st century, microscopy companies provide a variety of measurement techniques 52 for scientists. With the assistance of electron microscopy, plant scientists can observe 53 the cell surface and the detailed structure of organelles, and even decipher the structure 54 of proteins [2-4]. Optical microscopes, including upright and inverted microscopes, 55 provide powerful solutions for cellular observations as well [5, 6]. Since the application 56 of green fluorescent protein, confocal microscopy and various fluorescence related 57 techniques advanced the biological research field [7]. Furthermore, the methods to 58 analyze the corresponding data have been developed at a similar pace [8]. For larger 59 sample observation, stereoscopic microscopy offers non-destructive and detailed 60 insights to identify the tiny differences in betw...

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Section: Discussionmentioning

confidence: 99%

The microstructure investigation of plant architecture with X-ray microscopy

Zhang

Guo

Chen

et al. 2019

Preprint

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“…The storage ring continuously rotates the incident electron beam in a predetermined orbit and emits light every time the electron beam bends. Also, a beam line guides the light to the experimental device and other necessary equipment (Hwang et al, ; Lee & Lee, ; Lim et al, ). The X‐rays emitted from the beam line carry a high luminous flux and coherence, resulting in a clear image with an extended resolution (Hwang et al, ).…”

Section: Methodsmentioning

confidence: 99%

Synchrotron radiation‐based analysis of fatigue in dental restorative materials

Yoo

Kim

Min

et al. 2020

Microscopy Res & Technique

Self Cite

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“…In another study, CT was applied for investigation of earthworm for taxonomical purposes This result indicates that the stain uptake of sample was not similar for iodine and PTA. The anatomical structure of ant was investigated by Lee and Lee (2018) using syncrothrone CT. The ant was visualized in 3D and the obtained 3D images were used to 3D-print the ant structure.…”

Section: Phosphotungstic Acidmentioning

confidence: 99%

Evaluation of X‐ray tomography contrast agents: A review of production, protocols, and biological applications

Koç

Aslan

Kao

et al. 2019

Microscopy Res & Technique

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X‐ray computed tomography is a strong tool that finds many applications both in medical applications and in the investigation of biological and nonbiological samples. In the clinics, X‐ray tomography is widely used for diagnostic purposes whose three‐dimensional imaging in high resolution helps physicians to obtain detailed image of investigated regions. Researchers in biological sciences and engineering use X‐ray tomography because it is a nondestructive method to assess the structure of their samples. In both medical and biological applications, visualization of soft tissues and structures requires special treatment, in which special contrast agents are used. In this detailed report, molecule‐based and nanoparticle‐based contrast agents used in biological applications to enhance the image quality were compiled and reported. Special contrast agent applications and protocols to enhance the contrast for the biological applications and works to develop nanoparticle contrast agents to enhance the contrast for targeted drug delivery and general imaging applications were also assessed and listed.

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Usefulness of hard X‐ray microscope using synchrotron radiation for the structure analysis of insects

Cited by 12 publications

References 27 publications

The microstructure investigation of plant architecture with X-ray microscopy

The microstructure investigation of plant architecture with X-ray microscopy

Synchrotron radiation‐based analysis of fatigue in dental restorative materials

Evaluation of X‐ray tomography contrast agents: A review of production, protocols, and biological applications

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