Usefulness of Flow Cytometry in Diagnosis of IgG4-Related Ophthalmic Disease and Extranodal Marginal Zone B-Cell Lymphoma of the Ocular Adnexa

Kase, Satoru; Ishijima, Kan; Uraki, Takehiko; Suimon, Yuka; Suzuki, Yasuo; Kase, Manabu; Ishida, Susumu

doi:10.21873/anticanres.11913

Cited by 7 publications

(8 citation statements)

References 7 publications

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“…Recently, FCM has been reported as a useful examination for the diagnosis of ocular lymphoproliferative diseases. We and others have previously reported the profiles of several cell surface markers, showing that the rates of CD2, CD3, CD4, CD7, CD10 and CD23 positive cells were higher, and that CD19, CD20 and CD25 were lower in surgically excised tissues of IgG4-ROD compared to EMZL (5,6).…”

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confidence: 63%

Alteration of Cell Surface Markers CD38 and CD138 in Lymphoproliferative Disorders in the Ocular Adnexa

et al. 2020

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Background/Aim: CD38 is a cell surface marker commonly present in plasma cells and activated T cells, while CD138 is a representative plasma cell marker. The aim of this study was to describe the expression of cell surface markers including CD38 and CD138, in the tumors of patients with IgG4-related ophthalmic disease (IgG4-ROD) and extranodal marginal zone B-cell lymphoma (EMZL) of the ocular adnexa. Materials and Methods: Twenty-four consecutive patients of whom 12 had IgG4-ROD and 12 EMZL were enrolled in this study. Medical records were reviewed for flow cytometry (FCM) results on conventional T-cell markers, B-cell markers, CD38 and CD138. Results: Positive rates of T-cell markers, CD38 and CD138 were significantly higher in respectively). Conclusion: Our FCM results on CD38 and CD138 showed that the lymphocyte populations were different between IgG4-ROD and EMZL, which may reflect the different pathophysiology of the two diseases. Materials and MethodsMaterials. This is a retrospective observational study. The institutional review board in Hokkaido University Hospital approved this study

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confidence: 63%

Alteration of Cell Surface Markers CD38 and CD138 in Lymphoproliferative Disorders in the Ocular Adnexa

et al. 2020

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“…We have previously reported the usefulness of FCM findings using tumor tissues in the diagnosis of EMZL and FL. 8, 9 McKelvie et al analyzed the clinicopathological findings together with FCM of ocular adnexal lymphoproliferative disorders, including 8 cases with OA-DLBCL; however, the details of FCM findings are unknown. 14 Therefore, data acquisition in clinical practice and the usefulness of FCM in the diagnosis of OA-DLBCL has yet to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

Diffuse large b-cell lymphoma of the ocular adnexa with flow cytometry analysis and review of the literature

Mitamura

Kase

Suzuki

et al. 2022

European Journal of Ophthalmology

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Purpose The aim of this study was to evaluate the clinicopathological features and flow cytometry (FCM) of tumor tissues in ocular adnexal diffuse large B-cell lymphoma (DLBCL). Methods This retrospective, multicenter case study was designed to evaluate the clinical and immunohistochemical features of tumors. DLBCL was diagnosed based on histopathology, immunoglobulin (Ig) heavy chain gene rearrangement, and FCM in all surgically removed periocular tumor tissues. This study involved assessing percentages (%) of B-cell/T-cell markers, a natural killer cell marker, and cell-surface Ig kappa/lambda (κ/λ) expression measured by FCM analysis in tumor tissues. Results Eleven DLBCL patients (4 men and 7 women) with 11 tumors were enrolled in this study. The median age at the time of initial presentation was 73 years. The tumor cells were immunohistochemically positive for cluster of differentiation (CD) 20, while CD5 was negative in all 8 cases tested. At the time of ophthalmic diagnosis, two cases already showed systemic dissemination of DLBCL throughout the body. FCM of tumor tissues detected a high percentage of B-cell markers including CD19 and CD20 in all 11 tumors. One case with high CD10 levels in FCM was histologic transformation from follicular lymphoma. One case with a relatively low CD20 population involved a history of systemic treatments including intravenous rituximab. Conclusion Although caution should be exercised when interpreting the data, FCM is useful for not only supportive diagnosis complementary to immunohistochemistry, but also facilitates a better understanding of immunopathology including histologic transformation of follicular lymphoma to DLBCL in the ocular adnexa.

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“…Furthermore, we constructed ROC curves using a panel of miRNAs selected by the R package randomForest (version 4. [6][7][8][9][10][11][12][13][14] and compared them with a single miRNA. A value of 1.0 indicates that the features of the model completely discriminate two diseases, whereas a value of 0.5 indicates that the features contain disease information equal to that obtained by chance alone.…”

Section: Bioinformatic Tools For Target Prediction Function Enrichmementioning

confidence: 99%

“…In particular, the kappa/lambda ratio of a biopsy specimen is useful for verifying the monoclonality of malignant B cells. However, the wide range of kappa/lambda or lambda/kappa ratios (1.4–6.0) limits its specificity, and that test is not useful in identifying and distinguishing certain cases of orbital MALT lymphoma [ 7 , 8 , 9 ]. Additionally, Sato et al [ 10 ] detected IgG heavy-chain gene rearrangements in two of 17 cases of IgG4-ROD, further increasing the challenge in differentiating IgG4-ROD from orbital MALT lymphoma without specific analysis.…”

Section: Introductionmentioning

confidence: 99%

Distinctive Tissue and Serum MicroRNA Profile of IgG4-Related Ophthalmic Disease and MALT Lymphoma

Nezu

Usui

Asakage

et al. 2020

JCM

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The molecular pathogenesis of orbital lymphoproliferative disorders, such as immunoglobulin G4-related ophthalmic disease (IgG4-ROD) and orbital mucosa-associated lymphoid tissue (MALT) lymphoma, remains essentially unknown. Differentiation between the two disorders, which is important since the work-up and treatment can vary greatly, is often challenging due to the lack of specific biomarkers. Although miRNAs play an important role in the regulation of carcinogenesis and inflammation, the relationship between miRNA and orbital lymphoproliferative diseases remains unknown. We performed a comprehensive analysis of 2565 miRNAs from biopsy and serum specimens of 17 cases with IgG4-ROD, where 21 cases with orbital MALT lymphoma were performed. We identified specific miRNA signatures and their miRNA target pathways, as well as the network analysis for IgG4-ROD and orbital MALT lymphoma. Machine-learning analysis identified miR-202-3p and miR-7112-3p as the best discriminators of IgG4-ROD and orbital MALT lymphoma, respectively. Enrichment analyses of biological pathways showed that the longevity-regulating pathway in IgG4-ROD and the mitogen-activated protein kinase (MAPK) signaling pathway in orbital MALT lymphoma was most enriched by target genes of downregulated miRNAs. This is the first evidence of miRNA profiles of biopsy and serum specimens of patients with IgG4-ROD and orbital MALT lymphoma. These data will be useful for developing diagnostic and therapeutic interventions, as well as elucidating the pathogenesis of these disorders.

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Usefulness of Flow Cytometry in Diagnosis of IgG4-Related Ophthalmic Disease and Extranodal Marginal Zone B-Cell Lymphoma of the Ocular Adnexa

Abstract: Analyses of cell surface markers using FCM were useful in differentiating EMZL from IgG4-ROD/IOI. Sensitivity of Ig light chain restriction was relatively low in diagnosis of EMZL using FCM.

Cited by 7 publications

References 7 publications

Alteration of Cell Surface Markers CD38 and CD138 in Lymphoproliferative Disorders in the Ocular Adnexa

Alteration of Cell Surface Markers CD38 and CD138 in Lymphoproliferative Disorders in the Ocular Adnexa

Diffuse large b-cell lymphoma of the ocular adnexa with flow cytometry analysis and review of the literature

Distinctive Tissue and Serum MicroRNA Profile of IgG4-Related Ophthalmic Disease and MALT Lymphoma

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