Usefulness of flow cytometry in the diagnosis of mycosis fungoides

Oshtory, Shaheen; Apisarnthanarax, Narin; Gilliam, Anita C.; Cooper, Kevin D.; Meyerson, Howard

doi:10.1016/j.jaad.2007.01.017

Cited by 26 publications

(43 citation statements)

References 26 publications

(44 reference statements)

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“…Generalized erythroderma was present in three patients. Four were clinically classified as mycosis fungoides, two as [45][46][47]58 In this example, the cells are CD3dim, CD4( þ ) with a normal expression level, CD7( À ) and CD10( þ ). Note that CD10 is expressed only on the CD3dim cells (arrow).…”

Section: Resultsmentioning

confidence: 99%

“…[45][46][47] In all, 148 skin biopsies from 136 patients were sent to the Flow Cytometry Laboratory for evaluation. An abnormal T-cell population was identified in 50 of these patients.…”

Section: Patientsmentioning

confidence: 99%

“…45 Tissue specimens (skin and lymph node) were mechanically minced and incubated with 3.5 mg/ml of collagenase II (Sigma Chemical, St Louis, MO, USA) and 300 mg/ml of trypsin inhibitor, type II-O (Sigma) at 371C for 1 h with rocking, then filtered over a 40-mm nylon mesh. The isolated cells were washed once and resuspended in 2 ml of Minimum Essential Medium Eagle Spinner Modification (S-MEM) media and used for staining.…”

Section: Flow Cytometrymentioning

confidence: 99%

“…[45][46][47] An abnormal CD4( þ ) T-cell population was considered present if there was a discrete cluster of CD4( þ ) T cells (20 or more cells) based on FSC x SSC, CD45 x SSC or CD3 gating and cells within the gated population demonstrated a phenotypic abnormality defined as altered or absent expression of CD2, CD3, CD4, CD5, CD7, or CD26. [45][46][47] Molecular Analysis TCR gene rearrangement was assessed by PCR using oligonucleotide consensus primers specific for the T-cell receptor g gene variable and joining regions. Studies were performed on DNA extracted from fresh frozen tissue, tissue sections obtained from formalin-fixed, paraffin-embedded blocks, or peripheral blood.…”

Section: Flow Cytometrymentioning

confidence: 99%

“…Previously, we have had success in evaluating T cells isolated from skin biopsies using flow cytometry. [45][46][47] Here, we report CD10 expression on 11 T-cell lymphomas with primary cutaneous manifestations detected by flow cytometry. Although three of these cases exhibited features consistent with angioimmunoblastic T-cell lymphoma or peripheral T-cell lymphoma, the remaining (8 of 11) were best classified as mycosis fungoides or Sezary syndrome and expressed the follicular center helper T-cell-associated markers bcl-6 and PD-1.…”

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confidence: 99%

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Follicular center helper T-cell (TFH) marker positive mycosis fungoides/Sezary syndrome

et al. 2013

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Section: Resultsmentioning

confidence: 99%

“…[45][46][47] In all, 148 skin biopsies from 136 patients were sent to the Flow Cytometry Laboratory for evaluation. An abnormal T-cell population was identified in 50 of these patients.…”

Section: Patientsmentioning

confidence: 99%

Section: Flow Cytometrymentioning

confidence: 99%

Section: Flow Cytometrymentioning

confidence: 99%

mentioning

confidence: 99%

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Follicular center helper T-cell (TFH) marker positive mycosis fungoides/Sezary syndrome

et al. 2013

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Mycosis Fungoides

2009

Skin Lymphoma: The Illustrated Guide

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Usefulness of Flow Cytometry for the Detection of Cutaneous Localization in Malignant Hematologic Disorders

Maître

Le-Page

Comoz

et al. 2019

Cytometry Part B Clinical

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Background Histological evaluation of malignant hematologic involvement of the skin can be challenging and needs an extended immunohistochemistry panel. We assessed the ability of flow cytometry (FCM) to detect neoplastic cell subsets in skin biopsies as a useful tool that supplements the histological examination in a complementary way. Methods Two hundred and forty‐three consecutive skin biopsies were retrospectively analyzed between April 2012 and July 2017. Results Among them, 147 samples, corresponding to 128 patients, were analyzed at diagnosis. Eighty‐seven patients had erythrodermic inflammatory dermatoses, and 41 patients had cutaneous hematologic neoplasms. Cutaneous T‐cell lymphomas were the most frequent disorders, accounting for 70% of cases (29/41). Cutaneous B‐cell lymphoma was found in only 17% of cases (7/41) and immature hematologic malignancies in 5% (2/41). Three patients had secondary skin involvement. The sensitivity of FCM skin biopsy analysis was 78.1% (32/41). Among the 243 samples, 27 patients had mycosis fungoides (MF) or Sezary syndrome (SS) with available FCM data. A loss of CD26 expression was identified in 92% of cases of transformed MF or SS versus 40% of cases of non‐transformed MF (P = 0.0057 χ²). Among the 12 MF patients with negative CD26 expression, six progressed to SS versus none in the positive group (50% vs. 0% P = 0.0168 χ²). Conclusions FCM analysis of the skin biopsies is a sensitive method and a useful tool for improving the sensitivity of diagnosis of hematologic skin neoplasms. Among the MF patients, a loss of CD26 expression could be a marker of higher risk of progression. © 2019 International Clinical Cytometry Society

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Usefulness of flow cytometry in the diagnosis of mycosis fungoides

Cited by 26 publications

References 26 publications

Follicular center helper T-cell (TFH) marker positive mycosis fungoides/Sezary syndrome

Follicular center helper T-cell (TFH) marker positive mycosis fungoides/Sezary syndrome

Mycosis Fungoides

Usefulness of Flow Cytometry for the Detection of Cutaneous Localization in Malignant Hematologic Disorders

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