Usefulness of expanding the indications of early rescue intracytoplasmic sperm injection

Abstract

Purpose Early rescue intracytoplasmic sperm injection (ICSI) is often performed in cases in which not even a single oocyte has extruded a second polar body 6 h after insemination. We evaluated the usefulness of expanding the indications of early rescue ICSI to cases in which <80% of oocytes have extruded second polar bodies 6 h after insemination. Methods Early rescue ICSI was performed on oocytes that were denuded 2.5 h post‐insemination and whose extrusion of the second polar bodies had been examined 6 h post‐insemination with a PolScope. Results In vitro fertilization was performed on 24 496 oocytes of 4944 cycles, and 1438 cycles had <80% rate of the second polar body extrusion. Rescue ICSI was performed on 3933 oocytes. Three pronuclei (3PN) incidence of rescue ICSI was 3.0% in oocytes with ≥50% rate of the second polar body extrusion. With respect to the second polar body extrusion rate, no differences were observed in normal fertilization, blastocyst development, implantation, miscarriage, or live birth rates for rescue ICSI. Conclusion By expanding the indications of early rescue ICSI using the PolScope to cases in which <80% of oocytes have extruded the second polar bodies, many fertilized oocytes can be obtained without considerably increasing the 3PN rate.

Fertilization, pregnancy, and neonatal outcomes after IVF, rescue ICSI, and ICSI in unexplained infertility: A retrospective study

“…There are some controversial results regarding the indication of early R‐ICSI. Shibahara et al (2022) found that the expanding indication of early R‐ICSI to cycles, where the fertilization rate was <80%, is beneficial for rescuing unfertilized oocytes. However, Cao et al (2016) believed that early R‐ICSI is not recommended for cycles with a fertilization rate of >25%, as enough available pure IVF embryos are in these cycles.…”

Different Lengths of Diet Supplementation with 10% Flaxseed Alter the Hormonal Profile and the Follicular Fluid Fatty Acid Content of Fattening Gilts

“…At the same time, LA and AA content was decreased. High levels of EPA, DPA-3, and DHA were observed to increase the chance of achieving pregnancy [56] and improve embryo quality [57,58]. Moreover, studies in ruminants [59,60] revealed that lower LA content in FF was associated with improved folliculogenesis, number of follicles, ovulation rate, IVF (in vitro fertilization) performance, and number of fetuses.…”

Multimodal fluorescence microscope with fast adaptive polarimetry

“…Within the past five years, we can find hundreds of references to “OpenPolscope”, “Polscope” or “LC-Polscope” in the literature. As examples of recent publications in a broad spectrum of fields where these devices are used for quantitative polarization microscopy, we can cite studies within the liquid crystal community [10] , [11] , [12] , [13] , [14] , soft mater studies of in-vitro reconstitutions of cytoskeleton proteins [15] , [16] , intracellular studies [17] , [18] , [19] , and advanced functional materials [20] and biomaterials [21] . The measurements performed within this literature are mostly restricted to simple birefringence measurements and slow axis determination, and to quasi-stationary samples.…”