Usefulness of clinical magnetic resonance scanners for imaging experimental changes in laboratory rodents' central nervous system

Mystkowska, Dorota; Tutas, Anna; Jezierska-Woźniak, Katarzyna; Mikołajczyk, Anita; Bobek-Billewicz, Barbara; Jurkowski, Marek K.

doi:10.1016/j.poamed.2012.04.009

Cited by 5 publications

(4 citation statements)

References 15 publications

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“…It was also worthwhile to compare the sensitivity of various MR protocols in detecting mild BBB damage. A dose of 0.3 mmol/kg Gd‐DOTA was chosen for the DSC‐MRI in this study to obtain a good contrast‐to‐noise ratio, as commonly used in preclinical applications . While 0.1 mmol/kg CA is a typical dose for most preclinical DCE‐MRI studies, in our study K 2 from DSC‐MRI (<4 min) appeared to be more sensitive to the BBB‐damaged region than K trans from DCE‐MRI (<10 min), even with the same CA dose.…”

Section: Discussionmentioning

confidence: 74%

Pattern recognition analysis of dynamic susceptibility contrast (DSC)‐MRI curves automatically segments tissue areas with intact blood–brain barrier in a rat stroke model: A feasibility and comparison study

Jin

Han

Stoyanova

et al. 2019

Magnetic Resonance Imaging

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Background: The manual segmentation of intact blood-brain barrier (BBB) regions in the stroke brain is cumbersome, due to the coexistence of infarction, large blood vessels, ventricles, and intact BBB regions, specifically in areas with weak signal enhancement following contrast agent injection. Hypothesis: That from dynamic susceptibility contrast (DSC)-MRI alone, without user intervention, regions of weak BBB damage can be segmented based on the leakage-related parameter K 2 and the extent of intact BBB regions, needed to estimate K 2 values, determined. Study Type: Feasibility. Animal Model: Ten female Sprague-Dawley rats (SD, 200-250g) underwent 1-hour middle carotid artery occlusion (MCAO) and 1-day reperfusion. Two SD rats underwent 1-hour MCAO with 3-day and 5-day reperfusion. Field Strength/Sequence: 7T; ADC and T 1 maps using diffusion-weighted echo planar imaging (EPI) and relaxation enhancement (RARE) with variable repetition time (TR), respectively. dynamic contrast-enhanced (DCE)-MRI using FLASH. DSC-MRI using gradient-echo EPI. Assessment: Constrained nonnegative matrix factorization (cNMF) was applied to the dynamic ΔR * 2 -curves of DSC-MRI (<4 min) in a BBB-disrupted rat model. Areas of voxels with intact BBB, classified by automated cNMF analyses, were then used in estimating K 1 and K 2 values, and compared with corresponding values from manually-derived areas. Statistical Tests: Mean AE standard deviation of ΔT 1 -differences between ischemic and healthy areas were displayed with unpaired Student's t-tests. Scatterplots were displayed with slopes and intercepts and Pearson's r values were evaluated between K 2 maps obtained with automatic (cNMF)-and manually-derived regions of interest (ROIs) of the intact BBB region. Results: Mildly BBB-damaged areas (indistinguishable from DCE-MRI (10 min) parameters) were automatically segmented. Areas of voxels with intact BBB, classified by automated cNMF, matched closely the corresponding, manually-derived areas when respective areas were used in estimating K 2 maps (Pearson's r = 0.97, 12 slices). Data Conclusion: Automatic segmentation of short DSC-MRI data alone successfully identified areas with intact and compromised BBB in the stroke brain and compared favorably with manual segmentation.

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Section: Discussionmentioning

confidence: 74%

Pattern recognition analysis of dynamic susceptibility contrast (DSC)‐MRI curves automatically segments tissue areas with intact blood–brain barrier in a rat stroke model: A feasibility and comparison study

Jin

Han

Stoyanova

et al. 2019

Magnetic Resonance Imaging

View full text Add to dashboard Cite

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“…Scientific works show that MRI scanners potentially contribute to the more common use in animals and humans experimental research. 22,23 The spatial relations of the presented parts are often not visible or distorted by projection. Interaction and manipulation become complicated for untrained operators and may raise frustration.…”

Section: Discussionmentioning

confidence: 99%

Interactive CT/MRI 3D Fusion for cerebral system analysis and as a preoperative surgical strategy and educational tool

et al. 2021

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Introduction: The development of systems that merge existing technologies with gathered data may bring some spectacular effects that are usable both in preoperative and educational processes. Augmented reality (AR) is one of the key aspects of the new medical approach. Newly fused data sets draw from it and give users a better overall experience. Aim: The main goal of this study was to enable the interactive presentation of patients’ CT and MRI combined data with the incorporation of AR tools considering the accuracy of the data with an emphasis on vascular structures. Material and methods: The registration method, reconstruction of the vascular system using tubular structures, and error analysis using surface distance measurements results were used in the system to provide accurate combined information about bony structures from CT volume and vascular objects and cerebral vessels from MRI. Results and discussion: The strategies concern a series of CTI volumes that could be used to analyze bony surgical procedures. The methods are preferred, especially to the most complicated and individually modified bony structures of the skull. Removing, replacing, or modifying these bony structures or elements of the skull could be used as an analysis of operating procedures at the particular stages of the operation during neurosurgical or otolaryngological techniques. Conclusions: Presented study regarded to an innovative system consisting of a CT and MRI datasets fusion. The distance analysis of the segmented vascular model and proposed method for stabilization of the human head combined with virtual sculpting technique. In conclusion, it was meaningful in many aspects of the scientific-technological merge.

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“…Using MRI for animal research provides a wealth of information regarding an animal's condition without the need for dissection [6], decreasing the number of animals needed for an experimental protocol by allowing repeated measures on the same animal. MRI is also a relatively safe technique compared to CT as MRI does not require radiation to produce images [7].…”

Section: Introductionmentioning

confidence: 99%

Design and 3D-printing of MRI-compatible cradle for imaging mouse tumors

et al. 2021

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Background The ability of 3D printing using plastics and resins that are magnetic resonance imaging (MRI) compatible provides opportunities to tailor design features to specific imaging needs. In this study an MRI compatible cradle was designed to fit the need for repeatable serial images of mice within a mouse specific low field MRI. Methods Several designs were reviewed which resulted in an open style stereotaxic cradle to fit within specific bore tolerances and allow maximum flexibility with interchangeable radiofrequency (RF) coils. CAD drawings were generated, cradle was printed and tested with phantom material and animals. Images were analyzed for quality and optimized using the new cradle. Testing with multiple phantoms was done to affirm that material choice did not create unwanted image artifact and to optimize imaging parameters. Once phantom testing was satisfied, mouse imaging began. Results The 3D printed cradle fit instrument tolerances, accommodated multiple coil configurations and physiological monitoring equipment, and allowed for improved image quality and reproducibility while also reducing overall imaging time and animal safety. Conclusions The generation of a 3D printed stereotaxic cradle was a low-cost option which functioned well for our laboratory.

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Usefulness of clinical magnetic resonance scanners for imaging experimental changes in laboratory rodents' central nervous system

Cited by 5 publications

References 15 publications

Pattern recognition analysis of dynamic susceptibility contrast (DSC)‐MRI curves automatically segments tissue areas with intact blood–brain barrier in a rat stroke model: A feasibility and comparison study

Pattern recognition analysis of dynamic susceptibility contrast (DSC)‐MRI curves automatically segments tissue areas with intact blood–brain barrier in a rat stroke model: A feasibility and comparison study

Interactive CT/MRI 3D Fusion for cerebral system analysis and as a preoperative surgical strategy and educational tool

Design and 3D-printing of MRI-compatible cradle for imaging mouse tumors

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