Usefulness of Brush Cytology Combined With Pancreatic Juice Cytology in the Diagnosis of Pancreatic Cancer

Yamaguchi, Taketo; Shirai, Yoshihiko; Nakamura, Nami; Sudo, Kentaro; Nakamura, Kazuyoshi; Hironaka, Shuichi; Hara, Taro; Denda, Tadamichi

doi:10.1097/mpa.0b013e31825d60fc

Cited by 43 publications

(28 citation statements)

References 21 publications

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“…Pancreatic cancer (PC) is regarded as a lethal malignancy with an extremely low 5-year survival rate (<5%) [1,2]. Due to the hidden nature of the symptoms, early diagnosis is still a challenge in the treatment of pancreatic cancer, and only 15%–20% patients with pancreatic cancer are considered to have resectable disease when first diagnosed [3].…”

Section: Introductionmentioning

confidence: 99%

“…However, the invasive procedure may result in unexpected complications, including infection, bleeding, pancreatic fistula, bile leakage, etc. Besides, the sensitivity of imaging-guided biopsy tests for pancreatic cancer is only 80% [2] due to epithelial contamination or tumor dislocation. Currently, carbohydrate antigen 19-9 (CA19-9) is the most popular biomarker and has been commonly used as a prognostic indicator in pancreatic cancer patients [4,5].…”

Section: Introductionmentioning

confidence: 99%

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Detection of Circulating Tumor Cells Using Negative Enrichment Immunofluorescence and an In Situ Hybridization System in Pancreatic Cancer

Qin

et al. 2017

IJMS

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Pancreatic cancer (PC) is the most lethal type of gastrointestinal cancer, and early detection and monitoring is an urgent problem. Circulating tumor cells (CTCs) are emerging as a non-invasive biomarker for tumor detection. However, the low sensitivity is a main problem in the traditional CellSearch System for detecting CTCs, especially in patients with PC. In this study, we used negative enrichment (NE), immunofluorescence and in situ hybridization (FISH) of chromosome 8 (NE-iFISH) to capture and identify CTCs in PC patients. We showed that the NE-iFISH system exhibited a dramatically high detection rate of CTCs in PC patients (90%). The diagnostic rate of PC reached 97.5% when combining CTCs ≥ 2 and carbohydrate antigen 19-9 (CA19-9) > 37 µmol/L. The 1-year survival in the group of CTCs < 3 was significantly higher than that of CTCs ≥ 3 (p = 0.043). In addition, we analyzed the role of chromosomal instability in CTCs detection. The group of triploid (three hybridization signals of chromosome 8) CTCs ≥ 3 showed a shorter 1-year survival (p = 0.0279) and overall survival (p = 0.0188) than the group with triploid CTCs < 3. Importantly, the triploid CTC number but not the overall CTC counts could be a predictor of chemo-sensitivity. Moreover, circulating tumor microembolus (CTMs) were found in stage IV patients, and were positively related to the poor response to chemotherapy. In conclusion, the NE-iFISH system significantly improved the positive detection rate of CTCs and triploid CTC could be used to predict prognosis or the response to the chemotherapy of PC patients. CTM is a potential indicator of the chemotherapeutic effect in advanced PC patients.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of Circulating Tumor Cells Using Negative Enrichment Immunofluorescence and an In Situ Hybridization System in Pancreatic Cancer

Qin

et al. 2017

IJMS

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“…There are significant risks of pancreatitis with ductal brushing that can be reduced by placing a stent at the conclusion of the ERCP . The tissue yield of brush cytology of pancreatic duct can be improved by stricture dilation …”

Section: Pancreasmentioning

confidence: 99%

Techniques for cytologic sampling of pancreatic and bile duct lesions

Brugge

DeWitt

Klapman

et al. 2014

Diagnostic Cytopathology

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The Papanicolaou Society of Cytopathology has developed a set of guidelines for pancreatobiliary cytology including indications for endoscopic ultrasound guided fine-needle aspiration biopsy, techniques of the endoscopic retrograde cholangiopancreatography, terminology and nomenclature of pancreatobiliary disease, ancillary testing, and postbiopsy management. All documents are based on the expertise of the authors, a review of the literature, discussions of the draft document at several national and international meetings over an 18-month period and synthesis of online comments of the draft document on the Papanicolaou Society of Cytopathology website [www.papsociety.org]. This document presents the results of these discussions regarding the use of ancillary testing in the cytological diagnosis of biliary and pancreatic lesions. This document summarizes the current state of the art for techniques in acquiring cytology specimens from the biliary tree as well as solid and cystic lesions of the pancreas.

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“…[27] The tissue yield of brush cytology of pancreatic duct can be improved by stricture dilation. [28]…”

Section: Pancreasmentioning

confidence: 99%

Techniques for cytologic sampling of pancreatic and bile duct lesions: The Papanicolaou Society of Cytopathology Guidelines

et al. 2014

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The Papanicolaou Society of Cytopathology has developed a set of guidelines for pancreatobiliary cytology, including indications for endoscopic ultrasound guided fine-needle aspiration biopsy, techniques of the endoscopic retrograde cholangiopancreatography, terminology and nomenclature of pancreatobiliary disease, ancillary testing, and postbiopsy management. All documents are based on the expertise of the authors, a review of literature, discussions of the draft document at several national and international meetings over an 18 month period and synthesis of online comments of the draft document on the Papanicolaou Society of Cytopathology website [www.papsociety.org]. This document presents the results of these discussions regarding the use of sampling techniques in the cytological diagnosis of biliary and pancreatic lesions. This document summarizes the current state of the art for techniques in acquiring cytology specimens from the biliary tree as well as solid and cystic lesions of the pancreas.

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Usefulness of Brush Cytology Combined With Pancreatic Juice Cytology in the Diagnosis of Pancreatic Cancer

Abstract: Diagnosis of pancreatic cancer based on BC combined with PJC after brushing was more reliable than PJC before brushing or BC.

Cited by 43 publications

References 21 publications

Detection of Circulating Tumor Cells Using Negative Enrichment Immunofluorescence and an In Situ Hybridization System in Pancreatic Cancer

Detection of Circulating Tumor Cells Using Negative Enrichment Immunofluorescence and an In Situ Hybridization System in Pancreatic Cancer

Techniques for cytologic sampling of pancreatic and bile duct lesions

Techniques for cytologic sampling of pancreatic and bile duct lesions: The Papanicolaou Society of Cytopathology Guidelines

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