Usefulness of Beta-Catenin Immunostaining for the Differential Diagnosis of Solid-Pseudopapillary Neoplasm of the Pancreas

Tanaka, Yukichi; Notohara, Kenji; Kato, Keisuke; Ijiri, Rieko; Nishimata, Shigeo; Miyake, Tetsumi; Fukunaga, Masao; Horisawa, Minoru; Nakatani, Yukio

doi:10.1097/00000478-200206000-00023

Cited by 33 publications

(13 citation statements)

References 6 publications

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“…These impair normal phosphorylation by GSK-3β and subsequent ubiquitin-mediated degradation of β-catenin protein. Indeed, we (this study) and other groups [1,25,26] have demonstrated strong accumulation of β-catenin protein in the nuclei and cytoplasm of the tumor cells in SPN. In this study, 100% (14 of 14) of the SPNs showed intense, widespread nuclear and cytoplasmic accumulation of β-catenin protein (Table 3) and 86% (12 of 14) contained β-catenin gene mutations caused by exchange or deletion of the amino acids at GSK-3β phosphorylation sites or in their flanking region (Table 4).…”

Section: Discussionsupporting

confidence: 70%

“…Constitutive production of cyclin D1, one of the intranuclear targets of the β-catenin complex, was also frequently apparent in these SPNs [1,16]. We (this study) and others [5,26] have conversely demonstrated the absence of nuclear accumulation of β-catenin protein and mutations in exon 3 of the β-catenin gene in PETs, even though membranous localization (and rarely cytoplasmic accumulation) of β-catenin protein was observed.…”

Section: Discussionsupporting

confidence: 57%

“…Other groups have also demonstrated nuclear and cytoplasmic accumulation of β-catenin protein in the majority of tumor cells in all SPNs tested [1,25,26], while 83% (15/18) [25] to 90% (18/20) [1] of these SPNs had mutations in the β-catenin gene at GSK-3β phosphorylation sites or in their flanking region. Constitutive production of cyclin D1, one of the intranuclear targets of the β-catenin complex, was also frequently apparent in these SPNs [1,16].…”

Section: Discussionmentioning

confidence: 89%

“…Mutations of β-catenin have been reported in various tumors [21]; these are thought to cause stabilization of β-catenin leading to constitutive activation of TCF/LEF-1-dependent transcription [7,20]. Genetic studies have recently shown that neither ductal adenocarcinomas nor PETs are associated with mutations in the β-catenin gene [5], whereas Tanaka et al [25,26] and Abraham et al [1] demonstrated that aberrrant accumulation of β-catenin protein in the tumor cell nuclei and activating β-catenin oncogene mutations occurred in almost all of the SPNs they tested. They concluded that almost all SPNs show alterations in the adenomatous polyposis coli (APC)/β-catenin pathway, even though K-ras, DPC4, and P-53 do not play a major role in the neoplastic progression of SPN [1].…”

Section: Introductionmentioning

confidence: 99%

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Solid–pseudopapillary neoplasms of the pancreas in men and women: do they differ?

Takahashi

Hiraoka²,

Onozato³

et al. 2006

Virchows Arch

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Solid-pseudopapillary neoplasms (SPNs) of the pancreas are uncommon and occur preferentially in young women. The question whether the features of SPNs occurring in men differ from those in women has not yet been studied. For a better understanding of the clinicopathological features of SPNs of both sexes, we studied a series of 14 tumors surgically resected at a Japanese hospital within a period of 14 years. This series was composed of seven men and seven women. All these SPNs demonstrated nuclear and cytoplasmic accumulation of beta-catenin protein in immunohistochemistry and 86% of them had activating mutations of beta-catenin gene. No pancreatic neuroendocrine tumors showed such immunohistochemical findings and genetic alterations. In our series, most SPNs in women showed encapsulation by thick fibrous tissue and massive degenerative changes. Most SPNs in men exhibited solid components without prominent degenerative changes, even though they were of a similar size to those in women. These findings suggest that SPNs in men tend to be a solid mass with slower progression of degenerative changes during their growth compared to that in women. Nuclear accumulation of beta-catenin appears to be a useful marker of SPN, which allows male SPNs to be correctly diagnosed despite their less typical features.

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Section: Discussionsupporting

confidence: 70%

Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Solid–pseudopapillary neoplasms of the pancreas in men and women: do they differ?

Takahashi

Hiraoka²,

Onozato³

et al. 2006

Virchows Arch

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show abstract

“…Proposed cells of origin include pancreatic exocrine or endocrine cells, pluripotent stem cells, or cells from primitive gonadal tissues [5]. Mutations in beta-catenin are frequently present [6,7].…”

Section: Discussionmentioning

confidence: 99%