Usefulness of Aqueous Humor Analysis for the Diagnosis of Posterior Uveitis

Rothová, Aniki; Boer, Joke H. de; Loon, Ninette H. ten Dam-van; Postma, Gina; Visser, Lenneke de; Zuurveen, Stephanie J.; Schuller, Margje; Weersink, A. J. L.; Loon, Anton M. van; Groot-Mijnes, Jolanda D.F. de

doi:10.1016/j.ophtha.2007.05.014

Cited by 156 publications

(133 citation statements)

References 18 publications

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“…In recent papers it has been suggested that aqueous sampling is highly sensitive, reporting a positive PCR in 86.4% of cases. 11,12 Reasons for the discrepancy between this and our findings are unclear but may include inadequate size of sample and problems with processing. A sample taken early in the course of the disease may contain less viral DNA than vitreous for PCR amplification.…”

Section: Discussioncontrasting

confidence: 81%

Acute retinal necrosis in the United Kingdom: results of a prospective surveillance study

Cochrane

Silvestri

McDowell

et al. 2012

Eye

118

170

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Section: Discussioncontrasting

confidence: 81%

Acute retinal necrosis in the United Kingdom: results of a prospective surveillance study

Cochrane

Silvestri

McDowell

et al. 2012

Eye

118

170

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“…Indeed, in a recent study it was demonstrated that in 29% of patients with posterior uveitis aqueous analysis revealed an infectious cause and change of treatment was required in 24%. 28 Although vitreous biopsies are recommended for these cases, this study suggests that aqueous humor analysis might be a valuable and less invasive additional diagnostic procedure also in immunocompromised patients. The comparison of positive results from aqueous and vitreous analysis in different types of uveitis would be required to determine which diagnostic approach is most valuable in a given clinical situation.…”

Section: Our Study Demonstrates That In a Majority Of Im-mentioning

confidence: 84%

Infectious Uveitis in Immunocompromised Patients and the Diagnostic Value of Polymerase Chain Reaction and Goldmann-Witmer Coefficient in Aqueous Analysis

Westeneng¹,

Rothová²,

Boer³

et al. 2007

American Journal of Ophthalmology

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112

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• PURPOSE: To establish the causes of uveitis in immunocompromised patients and to determine the contribution of polymerase chain reaction (PCR) and Goldmann-Witmer coefficient (GWC) analysis of aqueous humor in patients with an infectious etiology.• DESIGN: Retrospective case series of 56 consecutive immunocompromised patients with uveitis.• METHODS: All patients underwent full ophthalmologic examination and laboratory blood analysis for uveitis. Aqueous humor analyses were performed using PCR and GWC for cytomegalovirus (CMV), herpes simplex virus (HSV), varicella zoster virus (VZV), and Toxoplasma gondii.• RESULTS: Of 56 immunocompromised patients, 43 (77%), all posterior and panuveitis, had intraocular infections. Twenty-one (49%) had CMV, three (7%) had VZV, 11 (26%) had T. gondii, six (14%) had Treponema pallidum, and one (2%) each had Aspergillus and Candida. In AIDS patients, CMV was the most common cause. A strong correlation between AIDS and ocular syphilis was also observed (P ‫؍‬ .007). In non-AIDS immunocompromised patients, T. gondii was most frequently detected. Twenty-seven patients were examined by both PCR and GWC; five (18.5%) were positive by both assays, 15 (55.5%) were positive by PCR alone and seven (26%) by GWC alone. Viral infections were detected by PCR in 16 of 17 (94%) cases; T. gondii in four of 10 (40%) patients. Using GWC, a viral infection was diagnosed in three of 17 (18%) and T. gondii in nine of 10 (90%) cases.

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“…In this manner, it has high sensitivity and eliminates post-PCR steps, yielding results in less time. It has been utilized for the diagnosis of various ocular infections such as trachoma, keratitis by herpes virus and Acanthamoeba spp., adenoviral conjunctivitis and hemorrhagic conjunctivitis by coxsackievirus A24, adenoviral keratoconjunctivitis, and uveitis caused by herpes simplex virus, varicella zoster virus, cytomegalovirus, Toxoplasma gondii, and Treponema pallidum (37)(38)(39)(40)(41)(42)(43)(44)(45) . Although it has not yet been applied in the diagnosis of endophthalmitis, the application of real-time PCR could be advantageous.…”

Section: Future Perspectivesmentioning

confidence: 99%

Molecular biology applied to the laboratory diagnosis of bacterial endophthalmitis

Bispo

Höfling-Lima

Pignatari

2009

Arq. Bras. Oftalmol.

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Usefulness of Aqueous Humor Analysis for the Diagnosis of Posterior Uveitis

Cited by 156 publications

References 18 publications

Acute retinal necrosis in the United Kingdom: results of a prospective surveillance study

Acute retinal necrosis in the United Kingdom: results of a prospective surveillance study

Infectious Uveitis in Immunocompromised Patients and the Diagnostic Value of Polymerase Chain Reaction and Goldmann-Witmer Coefficient in Aqueous Analysis

Molecular biology applied to the laboratory diagnosis of bacterial endophthalmitis

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