Usefulness of a triple fluorochrome combination Merocyanine 540/Yo-Pro 1/Hoechst 33342 in assessing membrane stability of viable frozen-thawed spermatozoa from Estonian Holstein AI bulls

Hallap, Triin; Nagy, Szabolcs; Jaakma, Ülle; Johannisson, Anders; Rodriguez‐Martinez, Heriberto

doi:10.1016/j.theriogenology.2005.07.009

Cited by 83 publications

(69 citation statements)

References 29 publications

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“…No comments on possible causes of RB were made by the near about fifteen percent farmers during the survey. Heuwieser et al, 1997;Pursley et al, 1998;Yousuf et al, 2010a, Hallap et al, 2006and Morrel, 2006 were agreed within the results of present findings who reported the quality feed, semen quality, inadequate heat detection, timing of insemination, insemination technique and reproductive diseases in dairy cows were the main factors of RB. Garge et al (2008) reported that balanced feeding is the mandatory factor for successful breeding in dairy cattle production.…”

Section: Resultssupporting

confidence: 91%

Identification of possible causes of repeat breeding in dairy cows at Baghabari milk shed areas, Sirajgonj, Bangladesh

Islam¹,

Deb²,

Nurunnahar³

et al. 2017

Asian J. Med. Biol. Res

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The present study was conducted at milk pocket areas Sahjadpur Upazila under Sirajgonj district and Shathia and Bera Upazila under the Pabna district, respectively. A total number of one hundred ninety (190) dairy farmers were surveyed randomly selecting twenty farmers from each village. All data collected were statistically analyzed using Univariate General Linear Model (GLM) procedure of SPSS computer program. Lack of balanced feed was the highest cause of repeat breeding claimed by about sixty (60%) percent farmers in this study. The highest incidence of RB was obtained in LocalHolstein Friesian crosses (69%) and lowest in LocalSahiwal crosses (8%). From the study of present findings; it can be concluded that genotype, nutrient of feed, quality of semen, frequency of insemination and reproductive problems are the main possible causes for RB in the studied areas.

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Section: Resultssupporting

confidence: 91%

Identification of possible causes of repeat breeding in dairy cows at Baghabari milk shed areas, Sirajgonj, Bangladesh

Islam¹,

Deb²,

Nurunnahar³

et al. 2017

Asian J. Med. Biol. Res

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“…Considering this limitation, use of YO-PRO-1 in combination with SNARF-1 and ethidium homodimer has been tested in cryopreserved boar spermatozoa with good correlation with Annexin V/PI as a result. 49 Another alternative for assessing early changes was to use another triple fluorochrome combination of Merocyanine 540, YO-PRO-1 and Hoechst 33342, 50 proving effective for several species. 51 Acrosome integrity.…”

Section: Sperm Intactnessmentioning

confidence: 99%

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Hossain¹,

Johannisson²,

Wallgren³

et al. 2011

Asian J Androl

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108

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Flow cytometry is now a recognized methodology within animal spermatology, and has moved from being a research tool to become routine in the assessment of animal semen destined to breeding. The availability of 'bench-top' flow cytometers and of newer and versatile markers for cell structure and function had allowed the instrumentation to measure more sperm parameters, from viability to reactiveness when exposed to exogenous stimuli, and to increase our capabilities to sort spermatozoa for potential fertilizing capacity, or chromosomal sex. The present review summarizes the state of the art regarding flow cytometry applied to animal andrology, albeit keeping an open comparative intent. It critically evaluates the present and future capabilities of flow cytometry for the diagnostics of potential fertility and for the development of current reproductive technologies such as sperm freezing, sperm selection and sperm sorting. The flow cytometry methods will probably further revolutionize our understanding of the sperm physiology and their functionality, and will undoubtedly extend its application in isolating many uncharacterized features of spermatozoa. However, continuous follow-up of the methods is a necessity owing to technical developments and the complexity of mapping spermatozoa.

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“…As membrane fluidity increases, more M540 is able to intercalate into the membrane, thereby acting as a useful marker for membrane destabilization [5,11,[35][36][37][38][39]. Moreover, increased M540 staining occurs within a few minutes after exposure of boar sperm to bicarbonate, and much earlier than TP staining is able to detect capacitation in stallion sperm [5,35].…”

Section: Introductionmentioning

confidence: 99%

Validation of merocyanine 540 staining as a technique for assessing capacitation-related membrane destabilization of fresh dog sperm

Steckler¹,

Stout

Durandt

et al. 2015

Theriogenology

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The aim of this study was to determine whether flow cytometric evaluation of combined merocyanine 540 and Yo-Pro 1 staining (M540-YP) would identify viable dog sperm that had undergone membrane stabilization known to be associated with capacitation in other species, and whether such destabilization is detected earlier than when using the tyrosine phosphorylation and ethidium homodimer stain combination (TP-EH) with epifluorescence microscopy. Semen from nine dogs was collected and incubated in parallel in bicarbonate-free modified Tyrode"s medium (-BIC), in medium containing 15 mM bicarbonate (+BIC), in dog prostatic fluid (PF), and in phosphate buffered saline (PBS). Aliquots for staining were removed at various time points during incubation of up to 6 hours. Staining with M540-YP allowed the classification of dog sperm as viable without destabilized membranes, viable with 2 destabilized membranes, non-viable without destabilized membranes or non-viable with destabilized membranes. The percentage of viable sperm detected using EH (83.5 ± 1.37%; mean ± SEM) was higher than when using YP (66.7 ± 1.37%: P < 0.05; n=54 semen samples). On the other hand, M540-YP identified a higher percentage of viable sperm with destabilized membranes than TP-EH (75 ± 1.76% vs. capacitation was recorded during incubation in PBS. We conclude that YP identifies sperm committed to cell death earlier than EH, and that the M540-YP stain combination identifies membrane destabilization known to be associated with capacitation in other species earlier than the TP-EH stain combination.

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Usefulness of a triple fluorochrome combination Merocyanine 540/Yo-Pro 1/Hoechst 33342 in assessing membrane stability of viable frozen-thawed spermatozoa from Estonian Holstein AI bulls

Cited by 83 publications

References 29 publications

Identification of possible causes of repeat breeding in dairy cows at Baghabari milk shed areas, Sirajgonj, Bangladesh

Identification of possible causes of repeat breeding in dairy cows at Baghabari milk shed areas, Sirajgonj, Bangladesh

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Validation of merocyanine 540 staining as a technique for assessing capacitation-related membrane destabilization of fresh dog sperm

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