Usefulness of a new technique for hind limb immobilization in rats for the study of tendon healing

Abstract

To study wound repair in rat Achilles tendon, we utilized a novel surgical method for immobilizing only one hind limb. In this technique, the femoral vessels were preserved while the skin, muscles, nerves and the shaft for the femur were transected. The limb was then rotated and sutured to lie on the posterolateral flank of the animal. The recovery was prompt and the procedure affected the routine growth and metabolism of the animal minimally throughout the period of experimentation. In the immobilized limb, healing of the Achilles tendon was studied after subtotal severance in the midsection. The rats were killed at intervals of 1, 2, and 4 weeks postoperatively. After 1 week, the surface of the wound was covered by a thin layer of cells. A gap persisted underneath and showed fibrovascular proliferation at the margins. In 2 weeks, the gap was completely occupied by fibrovascular tissue that was beginning to align along the long axis of the tendon. By the 4th week, remodeling had occurred and the site of the wound had almost blended with the rest of the tendon. We conclude that this new technique of hind limb immobilization causes little stress on the animal, and it can be useful for the study of tendon healing.