Useful markers for detecting decreased serum antioxidant activity in hemodialysis patients

Soejima, Akinori; Kaneda, Fumika; Manno, Sumie; Matsuzawa, Naoki; Kouji, Hitoshi; Nagasawa, Toshihiko; Era, Seiichi; Takakuwa, Yuichi

doi:10.1053/ajkd.2002.32787

Cited by 31 publications

(25 citation statements)

References 21 publications

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“…A decreased serum-scavenging activity against hydroxyl radicals in hemodialysis patients is demonstrated by electron-spin resonance and spin-trapping methods [13] . We previously reported the favorable effect of hemodialysis on decreased serum antioxidant activity by a novel bioassay of markers of erythrocyte membrane lipid peroxidation [6] . The levels of erythrocyte membrane lipid peroxidation products (thiobarbituric acid-reactive substances; TBARS) in predialysis uremic patients are signifi cantly higher than those in patients undergoing hemodialysis [7] .…”

Section: Discussionmentioning

confidence: 99%

“…An important physiological function of serum albumin is the maintenance of the redox state of the extracellular milieu via the mercapt-nonmercapt conversion. We have previously reported that the serum scavenging activity against erythrocyte membrane lipid peroxidation decreases in patients with renal failure and that this pathological condition is improved by hemodialysis [6,7] . We studied the mercapt-nonmercapt conversion of serum albumin before and after a hemodialysis session.…”

Section: Introductionmentioning

confidence: 99%

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Alteration of Redox State of Human Serum Albumin before and after Hemodialysis

et al. 2004

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Background:Persistent hypoalbuminemia is a predictor of death in long-term maintenance hemodialysis patients, although cardiovascular diseases remain the leading cause of death. A decreased serum antioxidant activity in maintenance hemodialysis patients may contribute to increased oxidative damage, and may be associated with accelerated atherosclerotic changes. Methods:The aim of this study was to examine the redox state of human serum albumin in maintenance hemodialysis patients by high-performance liquid chromatography (HPLC) using a fluorescence detector. Results: HPLC of human serum albumin on a Shodex-Asahipak ES-502N column at pH 4.85 showed a clear resolution of human mercaptalbumin (HMA) and nonmercaptalbumin (HNA), which are the reduced and oxidized forms of human serum albumin, respectively. The mean ± SD percentage of the HMA fraction of human serum albumin was significantly lower in maintenance hemodialysis patients than in age-matched normal subjects. The percentage of HMA increased 3–5 h after starting the hemodialysis and then decreased to subnormal levels. Conclusion: This suggests that serum albumin may be a major extracellular antioxidant in maintenance hemodialysis patients, and that hemodialysis may rescue serum albumin reduction by inducing intermolecular sulfhydryl-disulfide exchange reaction.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Alteration of Redox State of Human Serum Albumin before and after Hemodialysis

et al. 2004

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“…Although total HNA constitutes 20-25% of plasma albumin in healthy subjects, 19,20 our patients showed significantly higher percentages of HNA (HNA-1 and HNA-2) on starting HD; this result is similar to the findings of previous reports. 17,20 In this crossover study, however, there were no significant differences between the results before and after EVAL treatment and between the results before and after CTA treatment (Figure 1). EVAL treatment for the 2-month period demonstrated no advantage in terms of HNA reduction.…”

Section: Plasma Predialysis Hna Proportions Did Not Improve With 2 Momentioning

confidence: 63%

“…Plasma levels of mercaptalbumin (HMA) and HNA were measured according to the method described previously, 17 with high-performance liquid chromatography (HPLC). The chromatograph was equipped with a fluorescence detection system that consisted of a model 717plus auto sampler, model 600 double-plunger pump, and model 2475 fluorescence detector (all from Waters, Tokyo, Japan).…”

Section: Nonmercaptalbumin Measurementmentioning

confidence: 99%

Ethylene-Vinyl Alcohol Copolymer Dialyzer Membrane Reduces Protein Oxidation in Hemodialysis Patients

Matsumoto

Mukai

Arihara³

et al. 2011

Renal Failure

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Background: Oxidative stress has been implicated in the cardiovascular complications that affect hemodialysis (HD) patients. Ethylene-vinyl alcohol copolymer (EVAL) dialyzer membrane induces less production of reactive oxygen species as compared to conventional dialyzers. We evaluated the impact of EVAL membrane on plasma protein oxidation in HD patients. Methods: HD patients treated with cellulose triacetate (CTA) dialyzers were selected. In the first study performed in a 2-month crossover design alternating between CTA and EVAL, nonmercaptalbumin and advanced oxidation protein products levels were measured in the predialysis blood from 10 subjects. In the second study, predialysis plasma myeloperoxidase levels were measured before and after a 2-week EVAL treatment on 12 patients. Results: Plasma advanced oxidation protein products levels were reduced after a 2-month EVAL treatment and increased again after CTA treatment, although the nonmercaptalbumin proportions were not affected significantly by the change in dialyzer membranes. The following study, a 2-week EVAL treatment, showed the decrease in myeloperoxidase levels immediately before HD. Conclusion: The frequent use of EVAL dialyzers has been shown to reduce protein oxidation, possibly through the suppression of circulating phagocytes. This novel biocompatible dialyzer is expected to protect cardiovascular mortality in HD patients.

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“…Therefore, management of the oxidation status of serum albumin is an important issue in cases of CKD and medicine therapy. Serum albumin can be separated into HMA and HNA by HPLC (31) and is used to determine the redox state under various pathophysiological conditions (32)(33)(34)(35). We also recently demonstrated by HPLC that serum albumin shows high levels of oxidation in HD patients compared with age-and gendermatched healthy subjects, and that HPLC analysis of serum albumin can be useful for the quantitative and qualitative evaluation of oxidative stress in HD patients (16).…”

Section: Discussionmentioning

confidence: 99%