Use Supravital Toluidine Blue Staining to Improve the Efficiency of Fine-Needle Aspiration Cytology Reporting in Comparison to Papanicolaou Stain.

Abstract

Objective:To see the efficiency, adequacy and accuracy of toluidine blue stained smears of FNAC of Breast thyroid and salivary glands swelling with comparison to conventional stained FNAC smears with Papanicolaou.Methods:A total of 114 aspirates from various sites were included in the study. The smears were stained with toluidine blue and conventional Papanicolaou stain and the cytomorphology of both the smears were compared. The values were tabulated and statistical tests of significance was applied.Results:Of the 114 aspirates included in our study the diagnostic accuracy by using papanicolaou was 78%, while it was upto 100% with supravital toluidine blue stained smears. The percentage of inadequacy was reduced to just 25%. The observations were statistically significant. Breast 37/48 (77%) and Salivary glands 11/48 (23%) respectively. The most commonly used categorization of a five-tier system was used for reporting of breast cytology, with categories ranging from insufficient materials (C1), benign (C2), atypical (C3), suspicious of malignancy (C4), or (C5) frankly malignant. Most of breast lesions were benign 25 (67.56%). There were only 9 (24.32%) malignant cases followed by 2 cases of C-4 and one case of C-3. Benign thyroid lesion were more frequent comprising of 51 (72.27%) cases. One case (1.5%) of papillary carcinoma was found while 13 case were follicular lesions. There were 4 (36.4%) cases of pleomorphic adenoma and 3 (27.3) cases of non-specific sialadenitis. There was one case (9%) of each lesion for mucoepidermoid carcinoma, adenoidcytic carcinoma and benign cyst.Conclusion:Toluidine blue stained study of FNAC improves the diagnostic accuracy by minimizing the smearing and drying artifact, loss of cell sample during fixation and staining which influences the diagnostic accuracy.

Toluidine Blue Stain as a Rapid Onsite Tool for Preliminary Diagnosis in Imprint Smears of Bronchoscopic Biopsy: A Cytohistopathological Correlation

“…Moreover, the percentage of inadequacy was reduced to just 25%. 12 In 2012, Ammanagi et al showed that onsite TBS and screening improved the efficiency of FNAC reporting. 13 Various researchers like Miller et al, Onofre et al, Mashberg, and Hedge et al showed in their respective articles TBS as diagnostic adjunct in detection of asymptomatic oral squamous cell carcinoma and its utility in early detection of oral malignancies.…”

“…It is easily available, very cheap, cost-effective, and used for quick reporting. 12 Many of the studies with TBS were done on fine-needle aspiration cytology (FNAC) and oral mucosal lesions. TBS has also been used earlier by several researchers in their study for Rapid Onsite Evaluation (ROSE) on different sites like lymph nodes, thyroid, breast, and salivary glands.…”

Efficacy of toluidine blue staining in cervicovaginal cytology over conventional papanicolaou stain

“…3 TBS has been extensively used by various researchers as a vital stain for oral mucosal lesion and aspiration cytology reporting in recent years. [3][4][5] The literature shows, TBS is a practical, rapid, inexpensive and effective adjunct diagnostic tool. [4][5][6] Toluidine blue is supravital stain that accentuates good cytological and nuclear details and enables the three dimensional view of cells in wet mount film.…”

“…[3][4][5] The literature shows, TBS is a practical, rapid, inexpensive and effective adjunct diagnostic tool. [4][5][6] Toluidine blue is supravital stain that accentuates good cytological and nuclear details and enables the three dimensional view of cells in wet mount film. It is easily available, very cheap, cost effective and used for quick reporting.…”

“…It is easily available, very cheap, cost effective and used for quick reporting. 4,6 TBS is based on principle of metachromasia. Various applications of TBS are demonstration of connective tissue mucins, mast cell granules, amyloid, H.Pylori, endocrine cell granules, frozen section staining, etc.…”