Use of weathered diesel oil as a low-cost raw material for biosurfactant production

Abstract

This work aimed to investigate the capability of biosurfactant production by Staphylococcus hominis, Kocuria palustris and Pseudomonas aeruginosa LBI, using weathered diesel oil from a long-standing spillage as raw material. The effect of the culture media (Robert or Bushnell-Haas) and of the carbon source (spilled diesel oil or commercial diesel oil) on biosurfactant production was evaluated. Erlenmeyer flasks (250 mL) containing the cell broth were agitated (240 rpm) for 144 h at 27±2ºC. Biosurfactant production was monitored according to the De Nöuy ring method using a Krüss K6 tensiometer. Considering the possibility of intracellular storage of biosurfactant in the cell wall of the cultures S. hominis and K. palustris, experiments were also done applying ultrasound as a way to rupture the cells. For the conditions studied, the cultures did not indicate production of biosurfactants. Results obtained with a hydrocarbon biodegradability test based on the redox indicator 2,6-dichlorophenol indophenol showed that only the commercial diesel was biodegraded by the cultures

Investigation about the efficiency of the bioaugmentation technique when applied to diesel oil contaminated soils

“…By incorporating an electron acceptor such as DCPIP to the culture medium, it is possible to ascertain the ability of the microorganism to utilize the hydrocarbon substrate by observing the color change of DCPIP from blue (oxidized) to colorless (reduced) (Hanson et al, 1993). This technique has been employed in several works, for instance, Roy et al (2002), Cormack and Fraile (1997), Mariano et al (2008a) and Pirôllo et al (2008).…”

Renewable resources for biosurfactant production by yarrowia lipolytica

“…Molasses (Makkar and Cameotra, 1997), cassava wastewater (Nitschke and Pastore, 2002), olive oil mill effluent (Mercade and Mansera, 1994), animal fat (Desphande and Daniels, 1995), weathered diesel oil (Mariano et al, 2008 andSousa et al, 2012), waste frying oils (Vedaraman and Venkatesh, 2011), distillery residues and whey (Dubey and Juwarkar, 2001) have been studied as low cost substrates for microbial growth and biosurfactant production.…”

Production of bioemulsifiers by Yarrowia lipolytica in sea water using diesel oil as the carbon source

“…These authors adopted the criteria used by Habba et al (2000): a good biosurfactant decreases the surface tension to a value equal to or less than 40mN/m. In other studies, P. aeruginosa LBI was able to produce rhamnolipid biosurfactant in the presence of soapstock (Moraes et al, 2002), diesel oil (Mariano et al, 2008) and n-hexadecane, glycerol, babassu oil and paraffin oil (Santa-Anna et al, 2002).…”

“…The importance of monobasic phosphate potassium in bioemulsifier production in sea water is corroborated by studies that reported the production of emulsifiers by C. lipolytica in sea water diluted to 50%, supplemented with sources of nitrogen and phosphorus, using corn oil (Albuquerque et al, 2006) and babassu oil (Harrop-Vance et al, 2003) as carbon sources. Mariano et al (2008) evaluated the production of a biosurfactant by Staphylococcus hominis, Kocuria palustris and Pseudonomas aeruginosa LBI using a mineral medium in the presence of diesel oil (weathered and commercial) at 1, 5, 10, 20 and 30% v/v as a low cost substrate. The production of biosurfactant was monitored by the surface tension.…”