Use of Vpß.8 genes in splenic Lyt‐2<sup>+</sup> cytotoxic lymphocyte precursors reactive to bm1 or bm14 alloantigen in individual C57BL/6 mice

Reimann, Jörg; Bellan, Astrid

doi:10.1002/eji.1830161220

Cited by 6 publications

(3 citation statements)

References 37 publications

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“…Conversely, occurrence of Vb2.1 in the MLR-activated T cells was lower than that in the anti-CD3-activated T cells. We did not observe any extensive skewing toward particular single Vb genes reported in the allogeneic response toward HLA-DRw14 [14], H-2K bm1 and H-2D bm1 4 [15].…”

Section: Gene Occurrencecontrasting

confidence: 53%

T‐Cell Receptor Diversity Expressed by CD4⁺ T Cells Activated by Primary Allogeneic HLA‐DR Stimulation: Estimation of the Degree of CDR3 Diversity

1996

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In order to analyse the diversity of T-cell receptors (TCRs) expressed by the T-cell population activated by allogeneic HLA-DR stimulation, TCR beta cDNA was synthesized from mRNA of human CD4+ T cells that had been stimulated in a primary mixed lymphocyte reaction (MLR). The TCR beta cDNA was amplified by the polymerase chain reaction (PCR), subjected to bacterial cloning, and sequenced from V beta through J beta. Twenty-six different V beta and 10 different J beta segments were detected among 56 randomly selected cDNA clones. Occurrences of V beta 17.1 and J beta 1.5 were higher than those found in the CD4+ T-cell population activated with a CD3-specific antibody. A total of 53 different CDR3 sequences, two of them occurring more than once, were detected among the 56 cDNA clones. In order to estimate the degree of CDR3 diversity, amino acid similarity in the CDR3 region of the cDNA was calculated and compared with those of the anti-CD3-activated T-cell sequences as well as those of various published T-cell clone sequences, each directed to either alloantigens or single antigenic peptides. It was found that the similarity score among CDR3 sequences obtained from the MLR (56.4 +/- 10.3) was comparable to those of anti-CD3-activated T cells (55.7 +/- 10.7) and those of T-cell clones directed toward alloantigens (range, 48.4 +/- 12.4-59.4 +/- 13.1), but significantly smaller than those of T-cell clones directed toward single antigenic peptides such as those derived from myelin basic protein (75.6 +/- 17.9) and cytochrome c (76.9 +/- 20.5). These results provide quantitative proof that TCRs of T cells activated by primary allogeneic HLA-DR stimulation have a larger diversity than those recognizing single antigenic peptides.

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Section: Gene Occurrencecontrasting

confidence: 53%

T‐Cell Receptor Diversity Expressed by CD4⁺ T Cells Activated by Primary Allogeneic HLA‐DR Stimulation: Estimation of the Degree of CDR3 Diversity

1996

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“…This is, to our knowledge, the first structural analysis of rearranged TCR genes among human T cell clones in specific responses. Skewed V segment use among alloreactive T cells has also been observed in the mouse (25,(37)(38)(39), but very few V gene sequences were reported in these studies (25).…”

Section: Discussionmentioning

confidence: 95%

T cell receptor V beta gene usage in a human alloreactive response. Shared structural features among HLA-B27-specific T cell clones.

Bragado

Lauzurica

López

et al. 1990

The Journal of Experimental Medicine

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A strategy, based on using V beta family-specific oligonucleotides, was developed for specific amplification and direct sequencing of human TCR V beta genes. With this strategy, it was possible to undertake a structural analysis of TCRs from human T cell clones in specific responses. 12 HLA-B27-specific cytotoxic clones were examined. The results reveal a nonrandom use of V beta gene diversity in this alloreactive response in that: (a) the clones express a restricted number of V beta segments, including a subset of V beta families that are significantly more related to one another than to most other V beta families; (b) five of seven clones having a particular reaction pattern with HLA-B27 subtypes possess Alanine at the D-J junction; and (c) identical J beta segments are found associated in several instances with identical or highly homologous V beta gene segments. In addition, two new V beta 13 members are reported.

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“…While no single Va or V0 gene can explain the high frequency of I-Aó`" 12 -reactive T cells in B10 mice, the frequency of expression of VP genes in I-Ab m12 -reactive T cells does not simply reflect the random usage of V0 genes . Reimann and Bellan (48) have studied the use of the V08 family of TCR 0 chains in the response to the mutant class I alloantigens K b-' and Kbm14. Using F23.1, which recognizes the Vß8.1, 8.2, and 8.3 0 chain domains, to study CTL clones generated from 136 mice and reactive with Kbml or Kbm14, they found that 37°jo of Kbm'-reactive CTL clones and 51% of Kbm14 -reactive CTL clones express Vß8-encoded TCRs .…”

Section: Discussionmentioning

confidence: 99%

Molecular genetic analysis of 178 I-Abm12-reactive T cells.

Bill

Yagüe

Appel

et al. 1989

The Journal of Experimental Medicine

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We have studied the genetic diversity of the TCR repertoire to the murine alloantigen I-Abm12 by generating a panel of 178 C57BL/10-derived I-Abm12-reactive T cell hybridomas. The expression of V alpha and V beta gene families was examined in this panel and the frequency of expression of V beta, but not ofV alpha, gene families differed significantly from that observed in a companion panel of random C57BL/10-derived hybridomas. The V beta 5 gene family was expressed significantly less frequently while the V beta 14, V beta 15, and V beta 16 genes were expressed significantly more frequently in the panel of I-Abm12-reactive than in the panel of random hybridomas. The junctional regions (VJ alpha and VDJ beta) of TCR V alpha and V beta genes from selected I-Abm12-specific hybridomas were amplified using the polymerase chain reaction, and directly sequenced. Surprisingly, no conserved J alpha, D beta, J beta, or N region-encoded sequences among these selected I-Abm12-reactive TCRs were identified. Thus, the T cell response to an I-A alloantigen that differs by only three amino acid residues from the I-A molecule of the responding strain is genetically complex but nonrandom. We have estimated that the repertoire to this alloantigen is comprised of at least 37 different TCRs.

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Use of Vpß.8 genes in splenic Lyt‐2⁺ cytotoxic lymphocyte precursors reactive to bm1 or bm14 alloantigen in individual C57BL/6 mice

Cited by 6 publications

References 37 publications

T‐Cell Receptor Diversity Expressed by CD4⁺ T Cells Activated by Primary Allogeneic HLA‐DR Stimulation: Estimation of the Degree of CDR3 Diversity

T‐Cell Receptor Diversity Expressed by CD4⁺ T Cells Activated by Primary Allogeneic HLA‐DR Stimulation: Estimation of the Degree of CDR3 Diversity

T cell receptor V beta gene usage in a human alloreactive response. Shared structural features among HLA-B27-specific T cell clones.

Molecular genetic analysis of 178 I-Abm12-reactive T cells.

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Use of Vpß.8 genes in splenic Lyt‐2+ cytotoxic lymphocyte precursors reactive to bm1 or bm14 alloantigen in individual C57BL/6 mice

Cited by 6 publications

References 37 publications

T‐Cell Receptor Diversity Expressed by CD4+ T Cells Activated by Primary Allogeneic HLA‐DR Stimulation: Estimation of the Degree of CDR3 Diversity

T‐Cell Receptor Diversity Expressed by CD4+ T Cells Activated by Primary Allogeneic HLA‐DR Stimulation: Estimation of the Degree of CDR3 Diversity

T cell receptor V beta gene usage in a human alloreactive response. Shared structural features among HLA-B27-specific T cell clones.

Molecular genetic analysis of 178 I-Abm12-reactive T cells.

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Use of Vpß.8 genes in splenic Lyt‐2⁺ cytotoxic lymphocyte precursors reactive to bm1 or bm14 alloantigen in individual C57BL/6 mice

T‐Cell Receptor Diversity Expressed by CD4⁺ T Cells Activated by Primary Allogeneic HLA‐DR Stimulation: Estimation of the Degree of CDR3 Diversity

T‐Cell Receptor Diversity Expressed by CD4⁺ T Cells Activated by Primary Allogeneic HLA‐DR Stimulation: Estimation of the Degree of CDR3 Diversity