Use of virus‐induced gene silencing to characterize genes involved in modulating hypersensitive cell death in maize

Murphree, Colin A.; Kim, Saet-Byul; Karre, Shailesh; Samira, Rozalynne; Balint‐Kurti, Peter

doi:10.1111/mpp.12999

Cited by 12 publications

(14 citation statements)

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“…However, SGT1 only was up-regulated in the H_vs_VQ, but wasn't differentially expressed in the H_vs_V (Supplementary Table 3). The enhancement of HR induced by Rp1-D21 associated with suppression of the hydroxycinnamoyltransferase (HCT) and caffeoyl-CoA O-methyltransferase (CCoAOMT) genes in maize (Murphree et al, 2020). The consistent results also found in our data (Supplementary Table 3).…”

Section: Discussionsupporting

confidence: 91%

Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus

Hao

Huang

et al. 2021

Front. Microbiol.

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Tomato chlorosis virus (ToCV), is one of the most devastating cultivated tomato viruses, seriously threatened the growth of crops worldwide. As the vector of ToCV, the whitefly Bemisia tabaci Mediterranean (MED) is mainly responsible for the rapid spread of ToCV. The current understanding of tomato plant responses to this virus and B. tabaci is very limited. To understand the molecular mechanism of the interaction between tomato, ToCV and B. tabaci, we adopted a next-generation sequencing approach to decipher miRNAs and mRNAs that are differentially expressed under the infection of B. tabaci and ToCV in tomato plants. Our data revealed that 6199 mRNAs were significantly regulated, and the differentially expressed genes were most significantly associated with the plant-pathogen interaction, the MAPK signaling pathway, the glyoxylate, and the carbon fixation in photosynthetic organisms and photosynthesis related proteins. Concomitantly, 242 differentially expressed miRNAs were detected, including novel putative miRNAs. Sly-miR159, sly-miR9471b-3p, and sly-miR162 were the most expressed miRNAs in each sample compare to control group. Moreover, we compared the similarities and differences of gene expression in tomato plant caused by infection or co-infection of B. tabaci and ToCV. Taken together, the analysis reported in this article lays a solid foundation for further research on the interaction between tomato, ToCV and B. tabaci, and provide evidence for the identification of potential key genes that influences virus transmission in tomato plants.

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Section: Discussionsupporting

confidence: 91%

Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus

Hao

Huang

et al. 2021

Front. Microbiol.

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“…To test whether ZmCER9 suppressed Rp1-D21 in the endogenous maize system we employed a foxtail mosaic virus (FoMV)-based virus-induced gene silencing (VIGS) system 32 to silence ZmCER9 in maize B73 lines carrying Rp1-D21 (B73:Rp1-D21). The pFoMV-ZmCER9 construct carried a 277 bp insert fragment targeting the fourth transmembrane domain of ZmCER9 .…”

Section: Resultsmentioning

confidence: 99%

“…Biolistic inoculation of leaves with FoMV DNA constructs was carried out as previously described 32,66 . One-week old B73:Rp1-D21 plants were bombarded using a Biolistic PDS-1000/HE Particle Delivery System located in the NCSU Phytotron.…”

Section: Methodsmentioning

confidence: 99%

The maize E3 ligase ZmCER9 specifically targets activated NLRs for degradation

Karre

Selote

et al. 2021

Preprint

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The maize protein Rp1-D21, an autoactive derivative of the Rp1-D nucleotide-binding leucine rich repeat (NLR) disease resistance protein, triggers a spontaneous hypersensitive cell death defense response (HR). ZmCER9, a member of a class of E3 ligases involved in the endoplasmic reticulum associated degradation (ERAD) protein quality control system, is a suppressor of the Rp21-D21 HR phenotype. ZmCER9, an active E3 ligase, localizes to the ER, consistent with a role in ERAD. It physically interacts with and mediates the proteasome-dependent degradation of both Rp1-D21 and another autoactive Rp1-D derivate but neither interacts with nor affects the protein levels of three non-autoactive Rp1-D derivatives. ZmCER9 also suppresses the activity and directs the degradation of autoactive NLRs from Arabidopsis and barley. We describe a novel, and possibly general, mechanism that specifically degrades activated NLRs to suppress the defense response after activation.

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“…VIGS systems have been widely used in dicotyledonous plant species, including tomato (Cox et al 2019;Bao et al 2020), tobacco (Tang et al 2020), andArabidopsis (Calvo-Baltanás et al 2020). More recently, VIGS has been used in monocotyledonous species, including wheat (Yang et al 2020), barley (Gunupuru et al 2019), maize (Murphree et al 2020), and rice (Purkayastha et al 2010). Barley stripe mosaic virus (BSMV)-based VIGS has been developed for gene silencing in wheat, and has been reported for silencing genes related to growth and development (Gunupuru et al 2019;Murphree et al 2020), resistance to disease and insects (Eck et al 2010;Yousaf et al 2013;Yang et al 2020), and resistance to drought (Kuzuoglu-Ozturk et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…More recently, VIGS has been used in monocotyledonous species, including wheat (Yang et al 2020), barley (Gunupuru et al 2019), maize (Murphree et al 2020), and rice (Purkayastha et al 2010). Barley stripe mosaic virus (BSMV)-based VIGS has been developed for gene silencing in wheat, and has been reported for silencing genes related to growth and development (Gunupuru et al 2019;Murphree et al 2020), resistance to disease and insects (Eck et al 2010;Yousaf et al 2013;Yang et al 2020), and resistance to drought (Kuzuoglu-Ozturk et al 2012). The BSMV-VIGS system has also been used to investigate the functions of genes affecting wheat grain quality, especially starch and protein biosynthesis (Ma et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Virus-induced Silencing SBEIIa and SSIIa, Alone or Together, Increased the Amylose and Resistant Starch Contents in Spring Wheat

Li¹

2021

IJAB

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Foods rich in amylose and resistant starch (RS) have great potential to improve human health and lower the risk of noninfectious diseases. Common wheat (Triticum aestivumL.) is a major staple food crop with low RS content in the grains. The content of RS, preferentially derived from amylose, may be increased by suppressing amylopectin synthesis viasilencing the starch branching enzyme (SBE) II a or/and starch synthase (SS)IIa. In this study, SBEIIaand SSIIawere silenced separately and simultaneously using a barley stripe mosaic virus-virus-induced gene silencing (BSMV-VIGS) system. Compared with grains from control BSMV:00-inoculated spikes, grains from BSMV:SBEIIa-and BSMV:SSIIa-infected spikeshad fewer SBEIIa and SSIIa transcripts,together with increased amylose contents (18.62 and 24.48%, respectively) and RS contents (11.61 and 16.67%, respectively). Infection with BSMV:SBEIIa-SSIIa reduced SBEIIa and SSIIatranscript levels and increased the amylose and RS contents (32.02 and 22.33%, respectively). Thus, BSMV-VIGS is a useful tool for the rapid silencing of single or multiple starch synthase-related genes and BSMV shows great potential to study the functions of genes involved in starch biosynthesis or other processes/traits in developing grains.We showed that the SSIIagene plays an important role in the synthesis of amylose and RS and that the effects of simultaneously silencing SBEIIaand SSIIaon starch synthesis aregreater than those of single gene silencing. Our study lays the foundation for the molecular design-based breeding of high-amylose and high-RS wheat.© 2021 Friends Science Publishers

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Use of virus‐induced gene silencing to characterize genes involved in modulating hypersensitive cell death in maize

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 12 publications

References 87 publications

Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus

Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus

The maize E3 ligase ZmCER9 specifically targets activated NLRs for degradation

Virus-induced Silencing SBEIIa and SSIIa, Alone or Together, Increased the Amylose and Resistant Starch Contents in Spring Wheat

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