Use of VIRAZOLE� to eradicate odontoglossum ringspot virus from in vitro cultures of Cymbidium Sw.

Toussaint, André; Kummert, J.; Maroquin, C.; Lebrun, A.; Roggemans, J.

doi:10.1007/bf00042293

Cited by 16 publications

(8 citation statements)

References 8 publications

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“…In fact, the principal target of antiviral activity of these molecules is the inosine monophosphate dehydrogenase, an enzyme that catalyzes the conversion of inosine 5'-monophosphate (IMP) in xantosine 5'-monophosphate and is able to alter the pathway for the production of guanosine mono-di-and triphosphate. The presence of its inhibitor acts on this Weiland et al, 2004;Panattoni et al, 2006Panattoni et al, , 2007aSkiada et al, 2009bJames et al, 1997;Cieslinska, & Zawadzka, 1999;Cieslinska, 2002;O'Herlihy et al, 2003Janeckova, 1993Gabova, 1995;Chen & Li, 2001;Chen & Lu, 2000Kolbanova et al, 2004Iwanami & Ieki, 1994Cieslinska, 2003Conrad, 1991Green et al, 1992;Park et al, 1994;Kim et al, 1996;Truskinov & Rogozina, 1997;Faccioli & Zoffoli, 1998;Horackova, 1998;Nascimento et al, 2003;Fang et al, 2005Loi et al, 1991Schulze & Kluge, 1994Kim et al, 1994Xu & Niimi, 1999;Xu et al, 2000Ram et al, 2005Bertaccini et al, 2004Ramírez-Malagón et al, 2006Xu et al, 2004Lim et al, 1993;Toussaint et al, 1993;Fletcher et al, 1998;Freitas & Rezende, 1998;Yap et al, 1999;Fletcher & Fletcher, 200...…”

Section: Mechanism Of Actionmentioning

confidence: 99%

Review. Elimination of viruses in plants: twenty years of progress

Panattoni

Luvisi

Triolo

2013

Span. j. agric. res.

144

112

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To shed light on trends about elimination of viruses from plants, a bibliographic research was conducted to identify thermotherapy, chemotherapy and tissue culture trials published from 1991 through 2010. Among woody plants, grapevine, apple and peach are the most frequent targets of sanitation protocols because their health status is strictly regulated. Even if thermotherapy represents the preferred method for the host, grapevine viruses can also be eliminated with chemotherapy and tissue culture; apple viruses respond to chemotherapy as well. Although a similar trend was reported among herbaceous plants, chemotherapy was the most frequently used technique in potato. With regard to virus, thermotherapy was successfully applied against viruses belonging to 13 families and an unassigned genus. Instead, chemotherapy and tissue culture techniques eradicated viruses belonging to fewer families (nine). An interpretation of thermotherapy effects considers the new metabolic “pathways” triggered by the natural antiviral response emitted by the infected plant, with particular reference to virus-induced gene silencing. With regard to chemotherapy, several groups of antiviral drugs belong to inosine monophosphate dehydrogenase inhibitors, S-adenosylhomocysteine hydrolase inhibitors, neuraminidase inhibitors. Tissue culture, usually adopted to regenerate plantlets in biotechnological breeding programs, represents the less used tool for eliminate viruses from plants.

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Section: Mechanism Of Actionmentioning

confidence: 99%

Review. Elimination of viruses in plants: twenty years of progress

Panattoni

Luvisi

Triolo

2013

Span. j. agric. res.

144

112

View full text Add to dashboard Cite

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“…Chemotherapy was applied to embryo axes dissected from garlic cloves under aseptic conditions in an sterile air flow cabinet, and inoculated in 120 ml baby food jars (5 explants per jar) containing 20 ml of propagation medium supplemented with a filter-sterilized solution of Ribavirin (Virazole or 1-b-D-ribofuranosil-1, 2, 4-triazole-3-carboxamide; 205 lM) (Cassells, 1987;Toussaint et al 1993). When the explants had developed a new leaf (10-30 mm in length), this new more external leaf was dissected together with a thin layer of tissue from the base of the explant.…”

mentioning

confidence: 99%

Differential organ infection studies, potyvirus elimination, and field performance of virus-free garlic plants produced by tissue culture

Ramírez-Malagón

Pérez-Moreno

Borodanenko

et al. 2006

Plant Cell Tiss Organ Cult

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Presence of potyvirus in single garlic (Allium sativum L.) cloves from the same bulb, and in five single leaves excised from commercial fieldgrown individual plants was studied using ELISA. It was found that the viruses were not present in all organs of the same plant, since some cloves of the same bulb were infected with potyvirus but some others were potyvirus-free. Analyzed leaves from a given plant also exhibited irregular distribution of potyvirus. This study also aimed to obtain potyvirusfree plants from two commercial garlic cultivars (Taiwan and Chileno) using cloves subjected to thermotherapy, chemotherapy or meristematic dissection followed by in vitro culture. Thermotherapy (sequential treatment at 32°C for a week, 36°C for 2 weeks, and 38°C for 3 weeks) was found to affect survival of explants and 36.5% cloves from Taiwan and 26.8% from Chileno cultivars were recovered after the treatment. ELISA tests showed that 63% of the cloves of Taiwan that survived the treatment and 70.9% of Chileno explants were potyvirus-negative. Regarding chemotherapy (205 lM Ribavirin solution), the explants (cloves) survived, but only an average of 27.0-34.8% were negative for the presence of potyvirus. When meristematic dissection was applied, an average of 41.7% explants of Taiwan and 34.2% of Chileno survived the treatment, and approximately 64% of these explants from both cultivars were potyvirus-negative. Potyvirus-free garlic plants grown in field conditions showed longer stems with a major fresh and dry weight per bulb, and also exhibited a higher yield than non-treated plants.

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“…In apple shoot cultures ribavirin [virazole], an analog of guanosine has been used to eliminate Apple Chlorotic Leaf Spot Virus [ACLSV] in combination with tissue culture [9,10], used it in Cymbidium cultures to eradicate Odontoglossum Ringspot Virus [ORSV]. Potato viruses have been eliminated in potato meristem culture by using 2,4-dioxohexahydrol,3,5-triazine [11], and potato stem cuttings [12].…”

Section: Classical Chemicals Used As Virucidesmentioning

confidence: 99%

Plants as Antiviral Agents

Waziri¹

2015

J Plant Pathol Microbiol

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Viruses are microorganisms that infect all kinds of living organisms including plants, and cause remarkable lose in crop production. Although pesticides showed that they can protect plants from pest infections, there are no effective substances that can be used as potent virucides. Therefore, there is a continuous demand to produce chemicals in order to stop and cure viral infections in plants. However, toxicity and carcinogenicity issues were always attributed to chemical pesticides. Screening of natural products shined in the dark to find new safe virucides. The philosophy of selecting those plants is oriented towards plants that can protect themselves from viral infections. Plants have been reported as virus inhibitors and are able to prevent infection of viruses by inducing systemic resistance in noninfested parts of the plants, such as Boerhaavia diffusa, Clerodendrum aculeatum. Other plants defend themselves against virus infections; these plants contain ribosome inactivating proteins [RIPs], such as Phytolacca Americana, Mirabilis jalapa, Dianthus caryophullus. The methods used for extraction, separation, identification of those antiviral compounds are documented and discussed in this review.

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Use of VIRAZOLE� to eradicate odontoglossum ringspot virus from in vitro cultures of Cymbidium Sw.

Cited by 16 publications

References 8 publications

Review. Elimination of viruses in plants: twenty years of progress

Review. Elimination of viruses in plants: twenty years of progress

Differential organ infection studies, potyvirus elimination, and field performance of virus-free garlic plants produced by tissue culture

Plants as Antiviral Agents

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